Small Angle Scattering Biological Data Bank SASBDB

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24 hits found for Henrik Vinther Sørensen

SASDVZ9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 66 % D₂O

β-chitin nanofibers from squid pens experimental SAS data

β-chitin nanofibers from squid pens Kratky plot

Sample:	β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetic acid, 66% v/v D₂O, pH: 3.2
Experiment:	SANS data collected at D11, ILL on 2019 Jul 5

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDW22 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 80 % D₂O

Sample:	β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetic acid, 80% v/v D₂O, pH: 3.2
Experiment:	SANS data collected at D11, ILL on 2019 Jul 5

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDW32 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 100% D₂O

Sample:	β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetic acid, 100% v/v D₂O, pH: 3.2
Experiment:	SANS data collected at D11, ILL on 2019 Jul 5

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDW42 – 3 mg/ml β-chitin nanofibers from squid pens, SANS in 100 % D₂O

Sample:	β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetate, 99.7% v/v D₂O, pH: 5
Experiment:	SANS data collected at D11, ILL on 2020 Aug 18

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDW52 – 3 mg/ml β-chitin nanofibers from squid pens, SANS in 47 % D₂O

Sample:	β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetate, 47% v/v D₂O, pH: 5
Experiment:	SANS data collected at D11, ILL on 2019 Sep 21

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDW72 – Non-deuterated N-acetylglucosamine binding Protein A (H-GbpA) in 47 % D₂O, 2.5 mg/mL

GlcNAc-binding protein A experimental SAS data

Sample:	GlcNAc-binding protein A monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer:	100 mM NaCl, 20 mM Tris, 47% v/v D₂O, pH: 8
Experiment:	SANS data collected at D11, ILL on 2020 Aug 18

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

R_g^Guinier

25.0

SASDW82 – Perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O, 2.4 mg/mL

GlcNAc-binding protein A (perdeuterated) experimental SAS data

GlcNAc-binding protein A (perdeuterated) Kratky plot

Sample:	GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer:	100 mM NaCl, 20 mM Tris, 47% v/v D₂O, pH: 8
Experiment:	SANS data collected at D11, ILL on 2019 Sep 21

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

R_g^Guinier	3.7	nm
D_max	12.7	nm

SASDW92 – 10 mg/mL β-chitin nanofibers from squid pens with 2.5 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated) experimental SAS data

β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) Kratky plot

Sample:	β-chitin nanofibers from squid pens None, Squid GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM acetate, 47% v/v D₂O, pH: 5
Experiment:	SANS data collected at D11, ILL on 2020 Aug 18

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWA2 – 3 mg/mL β-chitin nanofibers from squid pens with 2.5 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O

Sample:	β-chitin nanofibers from squid pens None, Squid GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM acetate, 47% v/v D₂O, pH: 5
Experiment:	SANS data collected at D11, ILL on 2020 Aug 18

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWB2 – 3 mg/mL β-chitin nanofibers from squid pens with 2.5 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O - USANS

Sample:	β-chitin nanofibers from squid pens None, Squid GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM acetate, 47% v/v D₂O, pH: 5
Experiment:	SANS data collected at BT5 USANS, NIST Center for Neutron Research on 2020 Dec 4

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWC2 – 2.4 mg/mL β-chitin nanofibers from squid pens with 2 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O

Sample:	β-chitin nanofibers from squid pens None, Squid GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM acetate, 47% v/v D₂O, pH: 5
Experiment:	SANS data collected at D11, ILL on 2019 Sep 21

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWD2 – 2.4 mg/mL β-chitin nanofibers from squid pens with 0.5 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O

Sample:	β-chitin nanofibers from squid pens None, Squid GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM acetate, 47% v/v D₂O, pH: 5
Experiment:	SANS data collected at D11, ILL on 2019 Sep 21

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWE2 – 1.2 mg/mL β-chitin nanofibers from squid pens with 1 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 72 % D₂O

Sample:	β-chitin nanofibers from squid pens None, Squid GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM Acetate, 72% v/v D₂O, pH: 5
Experiment:	SANS data collected at D11, ILL on 2019 Sep 21

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWF2 – 0.48 mg/mL β-chitin nanofibers from squid pens with 1.2 mg/mL perdeuterated N-acetylglucosamine binding Protein A (D-GbpA) in 47 % D₂O

Sample:	β-chitin nanofibers from squid pens None, Squid GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM acetate, 47% v/v D₂O, pH: 5
Experiment:	SANS data collected at D11, ILL on 2019 Sep 21

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWG2 – 3 mg/mL β-chitin nanofibers from squid pens with 2.5 mg/mL non-deuterated N-acetylglucosamine binding Protein A (H-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A experimental SAS data

β-chitin nanofibers from squid pens GlcNAc-binding protein A Kratky plot

Sample:	β-chitin nanofibers from squid pens None, Squid GlcNAc-binding protein A monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM acetate, 47% v/v D₂O, pH: 5
Experiment:	SANS data collected at D11, ILL on 2020 Aug 18

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWH2 – 3 mg/mL β-chitin nanofibers from squid pens with a mixture of 1.25 mg/mL perdeuterated and 1.25 mg/mL non-deuterated N-acetylglucosamine binding Protein A (D-GbpA/H-GbpA) in 47 % D₂O

β-chitin nanofibers from squid pensGlcNAc-binding protein A (perdeuterated)GlcNAc-binding protein A experimental SAS data

β-chitin nanofibers from squid pens GlcNAc-binding protein A (perdeuterated) GlcNAc-binding protein A Kratky plot

Sample:	β-chitin nanofibers from squid pens None, Squid GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein GlcNAc-binding protein A monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM acetate, 47% v/v D₂O, pH: 5
Experiment:	SANS data collected at D11, ILL on 2020 Aug 18

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDWJ2 – 3 mg/mL β-chitin nanofibers from squid pens with a mixture of 0.625 mg/mL perdeuterated and 1.875 mg/mL non-deuterated N-acetylglucosamine binding Protein A (D-GbpA/H-GbpA) in 47 % D₂O

Sample:	β-chitin nanofibers from squid pens None, Squid GlcNAc-binding protein A (perdeuterated) monomer, 51 kDa Vibrio cholerae serotype … protein GlcNAc-binding protein A monomer, 51 kDa Vibrio cholerae serotype … protein
Buffer:	20 mM acetate, 47% v/v D₂O, pH: 5
Experiment:	SANS data collected at D11, ILL on 2020 Aug 18

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDVV9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in H₂O

Sample:	β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetic acid, pH: 3.2
Experiment:	SANS data collected at D11, ILL on 2019 Jul 5

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDVW9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 20 % D₂O

Sample:	β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetic acid, 20% v/v D₂O, pH: 3.2
Experiment:	SANS data collected at D11, ILL on 2019 Jul 5

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDVX9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 42 % D₂O

Sample:	β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetic acid, 42% v/v D₂O, pH: 3.2
Experiment:	SANS data collected at D11, ILL on 2019 Jul 5

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDVY9 – 10 mg/ml β-chitin nanofibers from squid pens, SANS in 47 % D₂O

Sample:	β-chitin nanofibers from squid pens None, Squid
Buffer:	20 mM acetate, 47% v/v D₂O, pH: 5
Experiment:	SANS data collected at D11, ILL on 2020 Aug 18

Tangled up in fibers: How a lytic polysaccharide monooxygenase binds its chitin substrate
Henrik Vinther Sørensen

SASDK42 – Chitin-binding protein CbpD (Pseudomonas aeruginosa), ESRF BM29 data

Chitin-binding protein CbpD experimental SAS data

Sample:	Chitin-binding protein CbpD monomer, 39 kDa Pseudomonas aeruginosa protein
Buffer:	15 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2020 Oct 1

The lytic polysaccharide monooxygenase CbpD promotes Pseudomonas aeruginosa virulence in systemic infection. Nat Commun 12(1):1230 (2021)
...Sørensen HV, Golten O, Bunæs AC, Mekasha S, Røhr ÅK, Kommedal E, Ludviksen JA, Arntzen MØ, Schmidt B, Zurich RH, van Sorge NM, Eijsink VGH, Krengel U, Mollnes TE, Lewis NE, Nizet V, Vaaje-Kolstad G

R_g^Guinier	3.2	nm
D_max	12.0	nm
Volume^Porod	64131	nm³

SASDJQ5 – Chitin-binding protein CbpD (Pseudomonas aeruginosa) at 24 °C

Sample:	Chitin-binding protein CbpD monomer, 39 kDa Pseudomonas aeruginosa protein
Buffer:	15 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at Bruker Nanostar with InCoatec Cu microsource, RECX, University of Oslo on 2019 Jul 19

R_g^Guinier	3.3	nm
D_max	12.1	nm
Volume^Porod	50	nm³

SASDJR5 – Chitin-binding protein CbpD (Pseudomonas aeruginosa) at 37°C

Sample:	Chitin-binding protein CbpD monomer, 39 kDa Pseudomonas aeruginosa protein
Buffer:	15 mM Tris-HCl 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at Bruker Nanostar with InCoatec Cu microsource, RECX, University of Oslo on 2019 Aug 14

R_g^Guinier	3.5	nm
D_max	15.0	nm
Volume^Porod	63	nm³