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2 hits found for Joshua Ramsay

SASDW97 – Recombination directionality factor (6H-RdfS) from M.japonicum bound to 40mer DNA

Recombination directionality factor RdfSattP_8 40-mer DNA experimental SAS data
OTHER model
Sample: Recombination directionality factor RdfS tetramer, 52 kDa Mesorhizobium japonicum R7A protein
attP_8 40-mer DNA dimer, 25 kDa DNA
Buffer: 150 mM Tris-HCl, 300 mM NaCl, 5% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 19
Structural basis for control of integrative and conjugative element excision and transfer by the oligomeric winged helix–turn–helix protein RdfS Nucleic Acids Research 53(6) (2025)
...Ramsay J
RgGuinier 3.8 nm
Dmax 13.8 nm
VolumePorod 134 nm3

SASDXJ8 – Hexahistidine-tagged Quorum sensing controller protein (QseC) bound to dual operator OL and OR DNA sites

Quorum sensing controller protein QseCQseC Operator OL+OR DNA experimental SAS data
DAMMIN model
Sample: Quorum sensing controller protein QseC tetramer, 44 kDa Mesorhizobium japonicum protein
QseC Operator OL+OR DNA monomer, 24 kDa DNA
Buffer: 50mM Tris, 300mM NaCl, 2.5% glycerol, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2020 Apr 24
Hexahistidine-tagged QseC bound to operator DNA sites
Joshua Ramsay
RgGuinier 3.2 nm
Dmax 12.6 nm
VolumePorod 70 nm3