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12 hits found for Obsil

SASDUC3 – Phosphorylated E3 ubiquitin-protein ligase Nedd4-2 in the absence of calcium

Isoform 5 of E3 ubiquitin-protein ligase NEDD4-like experimental SAS data
CORAL model
Sample: Isoform 5 of E3 ubiquitin-protein ligase NEDD4-like monomer, 110 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 1 mM EDTA, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17
Structural basis of ubiquitin ligase Nedd4-2 autoinhibition and regulation by calcium and 14-3-3 proteins Nature Communications 16(1) (2025)
...Obsil T, Obsilova V
RgGuinier 4.9 nm
Dmax 22.0 nm
VolumePorod 206 nm3

SASDUD3 – Phosphorylated E3 ubiquitin-protein ligase Nedd4-2 in the presence of calcium

Isoform 5 of E3 ubiquitin-protein ligase NEDD4-like experimental SAS data
Isoform 5 of E3 ubiquitin-protein ligase NEDD4-like Kratky plot
Sample: Isoform 5 of E3 ubiquitin-protein ligase NEDD4-like monomer, 110 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 1 mM TCEP, 1 mM CaCl2, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17
Structural basis of ubiquitin ligase Nedd4-2 autoinhibition and regulation by calcium and 14-3-3 proteins Nature Communications 16(1) (2025)
...Obsil T, Obsilova V
RgGuinier 4.9 nm
Dmax 22.5 nm
VolumePorod 201 nm3

SASDL56 – 14-3-3 zeta truncated at C-terminus

14-3-3 protein zeta/delta experimental SAS data
14-3-3 protein zeta/delta Kratky plot
Sample: 14-3-3 protein zeta/delta dimer, 53 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 13
A structural study of the cytoplasmic chaperone effect of 14-3-3 proteins on Ataxin-1. J Mol Biol :167174 (2021)
...Obsil T, Brunsveld L, Crabbe T, Yahya Zoghbi H, Ottmann C, Martin Davis J
RgGuinier 2.8 nm
Dmax 7.9 nm

SASDSJ7 – Human C-terminally truncated 14-3-3 gamma

14-3-3 protein gamma experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: 14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 3
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
...Obsilova V, Obsil T
RgGuinier 2.8 nm
Dmax 8.4 nm
VolumePorod 76 nm3

SASDLT3 – Ataxin-1 AXH-C

Ataxin-1 experimental SAS data
Ataxin-1 AXH-C Rg histogram
Sample: Ataxin-1 dimer, 55 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 13
A structural study of the cytoplasmic chaperone effect of 14-3-3 proteins on Ataxin-1. J Mol Biol :167174 (2021)
...Obsil T, Brunsveld L, Crabbe T, Yahya Zoghbi H, Ottmann C, Martin Davis J
RgGuinier 4.3 nm
Dmax 14.6 nm
VolumePorod 90 nm3

SASDSR8 – Calcium/calmodulin-dependent protein kinase kinase 1 (CaMKK1)

Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) experimental SAS data
Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) monomer, 46 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
...Obsilova V, Obsil T
RgGuinier 3.2 nm
Dmax 10.7 nm
VolumePorod 114 nm3

SASDL46 – 14-3-3zeta AXH-C complex

14-3-3 protein zeta/deltaAtaxin-1 AXH-C experimental SAS data
MULTIFOXS model
Sample: 14-3-3 protein zeta/delta dimer, 53 kDa Homo sapiens protein
Ataxin-1 AXH-C dimer, 55 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Oct 13
A structural study of the cytoplasmic chaperone effect of 14-3-3 proteins on Ataxin-1. J Mol Biol :167174 (2021)
...Obsil T, Brunsveld L, Crabbe T, Yahya Zoghbi H, Ottmann C, Martin Davis J
RgGuinier 4.8 nm
Dmax 19.8 nm

SASDSS8 – Phosphorylated Calcium/calmodulin-dependent protein kinase 1 (pCaMKK1)

Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) experimental SAS data
Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) monomer, 46 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
...Obsilova V, Obsil T
RgGuinier 3.0 nm
Dmax 10.5 nm
VolumePorod 97 nm3

SASDSQ7 – Calcium/calmodulin-dependent protein kinase kinase 2 (CaMKK2)

Calcium/calmodulin-dependent protein kinase kinase 2 experimental SAS data
MULTIFOXS model
Sample: Calcium/calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 3
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
...Obsilova V, Obsil T
RgGuinier 3.2 nm
Dmax 13.3 nm
VolumePorod 91 nm3

SASDST8 – Phosphorylated Calcium/calmodulin-dependent protein kinase 2 (pCaMKK2)

Calcium/calmodulin-dependent protein kinase kinase 2 experimental SAS data
Calcium/calmodulin-dependent protein kinase kinase 2 Kratky plot
Sample: Calcium/calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 Aug 17
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
...Obsilova V, Obsil T
RgGuinier 3.0 nm
Dmax 12.0 nm
VolumePorod 87 nm3

SASDS46 – Human phosphorylated calcium/calmodulin-dependent protein kinase kinase 1 (CaMKK1) bound to 14-3-3 protein gamma

Calcium/calmodulin-dependent protein kinase kinase 1 (M474L)14-3-3 protein gamma experimental SAS data
CORAL model
Sample: Calcium/calmodulin-dependent protein kinase kinase 1 (M474L) monomer, 46 kDa Homo sapiens protein
14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Oct 23
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
...Obsilova V, Obsil T
RgGuinier 3.3 nm
Dmax 10.8 nm
VolumePorod 152 nm3

SASDSX7 – Human phosphorylated CaMKK2 bound to 14-3-3 gamma

14-3-3 protein gammaCalcium/calmodulin-dependent protein kinase kinase 2 experimental SAS data
ALLOSMOD model
Sample: 14-3-3 protein gamma dimer, 54 kDa Homo sapiens protein
Calcium/calmodulin-dependent protein kinase kinase 2 monomer, 48 kDa Homo sapiens protein
Buffer: 50 mM Tris-HCl, 150 mM NaCl, 1 mM TCEP, 3% (w/v) glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Oct 23
14-3-3 protein inhibits CaMKK1 by blocking the kinase active site with its last two C-terminal helices. Protein Sci :e4805 (2023)
...Obsilova V, Obsil T
RgGuinier 3.7 nm
Dmax 13.2 nm
VolumePorod 157 nm3