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5 hits found for Raktim Roy

SASDSR7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the apo form without any substrates

Gramicidin S synthase 1 (W239S) experimental SAS data
BILBOMD model
Sample: Gramicidin S synthase 1 (W239S) monomer, 127 kDa Aneurinibacillus migulanus protein
Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14
Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy
RgGuinier 4.1 nm
Dmax 15.0 nm
VolumePorod 187 nm3

SASDSS7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the apo form in the presence of 4 molar excess of L-Tyrosine

Gramicidin S synthase 1 (W239S) experimental SAS data
Sample: Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein
Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14
Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy
RgGuinier 4.3 nm
Dmax 15.4 nm
VolumePorod 186 nm3

SASDST7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the apo form in the presence of 4 molar excess of L-Tyrosine and ATP analog (AMPPCP)

Gramicidin S synthase 1 (W239S) experimental SAS data
Sample: Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein
Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14
Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy
RgGuinier 4.2 nm
Dmax 16.0 nm
VolumePorod 186 nm3

SASDSU7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the holo form in the presence of 4 molar excess of L-Tyrosine

Gramicidin S synthase 1 (W239S) experimental SAS data
Sample: Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein
Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14
Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy
RgGuinier 4.1 nm
Dmax 17.5 nm
VolumePorod 181 nm3

SASDSV7 – Gramicidin Synthetase 1 (GrsA) W239S mutant in the holo form in the presence of 4 molar excess of L-Tyrosine and ATP analog (AMPPCP)

Gramicidin S synthase 1 (W239S) experimental SAS data
Sample: Gramicidin S synthase 1 (W239S) monomer, 126 kDa Brevibacillus brevis protein
Buffer: 20 mM Tris pH 7.5, 100 mM NaCl, 10 mM MgCl2, 2% glycerol, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2023 Mar 14
Resting state of GrsA is a combination of two constitutively inactive isoforms that manifest long-range allosteric effects in presence of aminoacid and ATP
Raktim Roy
RgGuinier 4.0 nm
Dmax 14.3 nm
VolumePorod 175 nm3