Search

  
Advanced search  

11 hits found for Snoj

SASDUN2 – DiTET-cc (coiled coil protein origami tetrahedron assembled from two cyclized chains)

cyRH1 (cyclic bitrigon from 6 coiled coil segments)cyRH2 (cyclic bitrigon from 6 coiled coil segments) experimental SAS data
SREFLEX model
Sample: cyRH1 (cyclic bitrigon from 6 coiled coil segments) monomer, 26 kDa synthetic construct protein
cyRH2 (cyclic bitrigon from 6 coiled coil segments) monomer, 26 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Mar 30
Preorganized cyclic modules facilitate the self-assembly of protein nanostructures Chemical Science (2024)
Snoj J, Lapenta F, Jerala R
RgGuinier 3.1 nm
Dmax 8.8 nm
VolumePorod 150 nm3

SASDUP2 – DiTET-nc (coiled coil protein origami tetrahedron assembled from one linear and one cyclized chain)

RH1 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments)cyRH2 (cyclic bitrigon from 6 coiled coil segments) experimental SAS data
SREFLEX model
Sample: RH1 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) monomer, 26 kDa synthetic construct protein
cyRH2 (cyclic bitrigon from 6 coiled coil segments) monomer, 26 kDa synthetic construct protein
Buffer: 50 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Aug 29
Preorganized cyclic modules facilitate the self-assembly of protein nanostructures Chemical Science (2024)
Snoj J, Lapenta F, Jerala R
RgGuinier 3.6 nm
Dmax 12.5 nm
VolumePorod 161 nm3

SASDUQ2 – DiTET-nn (coiled coil protein origami assembled from two linear chains)

RH1 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments)RH2 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) experimental SAS data
SREFLEX model
Sample: RH1 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) monomer, 26 kDa synthetic construct protein
RH2 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) monomer, 26 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 9
Preorganized cyclic modules facilitate the self-assembly of protein nanostructures Chemical Science (2024)
Snoj J, Lapenta F, Jerala R
RgGuinier 4.3 nm
Dmax 14.7 nm
VolumePorod 161 nm3

SASDUR2 – SB24-nnn (coiled coil protein origami irregular octahedron assembled from three linear chains)

SB6 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments)SB9b (Linear coiled-coil protein origami structure consisting of 9 coiled-coil forming segments)SB9c (Linear coiled-coil protein origami structure consisting of 9 coiled-coil forming segments) experimental SAS data
MODELLER model
Sample: SB6 (Linear coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) monomer, 26 kDa synthetic construct protein
SB9b (Linear coiled-coil protein origami structure consisting of 9 coiled-coil forming segments) monomer, 42 kDa synthetic construct protein
SB9c (Linear coiled-coil protein origami structure consisting of 9 coiled-coil forming segments) monomer, 41 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 Oct 25
Preorganized cyclic modules facilitate the self-assembly of protein nanostructures Chemical Science (2024)
Snoj J, Lapenta F, Jerala R
RgGuinier 4.8 nm
Dmax 16.1 nm
VolumePorod 443 nm3

SASDUS2 – SB24-ncn (coiled coil protein origami irregular octahedron assembled from 2 linear chains and 1 cyclic chain)

SB9b (Linear coiled-coil protein origami structure consisting of 9 coiled-coil forming segments)SB9c (Linear coiled-coil protein origami structure consisting of 9 coiled-coil forming segments)cySB6 (Cyclic coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) experimental SAS data
COOT model
Sample: SB9b (Linear coiled-coil protein origami structure consisting of 9 coiled-coil forming segments) monomer, 42 kDa synthetic construct protein
SB9c (Linear coiled-coil protein origami structure consisting of 9 coiled-coil forming segments) monomer, 41 kDa synthetic construct protein
cySB6 (Cyclic coiled-coil protein origami structure consisting of 6 coiled-coil forming segments) monomer, 26 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 9
Preorganized cyclic modules facilitate the self-assembly of protein nanostructures Chemical Science (2024)
Snoj J, Lapenta F, Jerala R
RgGuinier 4.7 nm
Dmax 14.7 nm
VolumePorod 428 nm3

SASDW37 – SSRD - Coiled-Coil Tetrahedron with Four Functional Domains (2x SpyCatcher, RFP, DCV)

TET12-2Sc-RFP-DCV experimental SAS data
MODELLER model
Sample: TET12-2Sc-RFP-DCV monomer, 109 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Sep 24
Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization. Small 21(30):e2502060 (2025)
...Snoj J, Škarabot M, Jerala R
RgGuinier 4.4 nm
Dmax 13.6 nm
VolumePorod 300 nm3

SASDW47 – SSRD-2xDRSt - Coiled-Coil Tetrahedron with Four Functional Domains (2x SpyCatcher, RFP, DCV) and Dual SpyCatcher-SpyTag Linkages with DCV-RFP-St Extension

TET12-2Sc-RFP-DCVDCV-RFP-StDCV-RFP-St experimental SAS data
SREFLEX model
Sample: TET12-2Sc-RFP-DCV monomer, 109 kDa synthetic construct protein
DCV-RFP-St monomer, 41 kDa synthetic construct protein
DCV-RFP-St monomer, 41 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Sep 24
Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization. Small 21(30):e2502060 (2025)
...Snoj J, Škarabot M, Jerala R
RgGuinier 5.1 nm
Dmax 15.4 nm
VolumePorod 480 nm3

SASDW57 – SSRD-2xDGSt - Coiled-Coil Tetrahedron with Four Functional Domains (2x SpyCatcher, RFP, DCV) and Dual SpyCatcher-SpyTag Linkages with DCV-GFP-St Extension

TET12-2Sc-RFP-DCVDCV-GFP-StDCV-GFP-St experimental SAS data
MODELLER model
Sample: TET12-2Sc-RFP-DCV monomer, 109 kDa synthetic construct protein
DCV-GFP-St monomer, 41 kDa synthetic construct protein
DCV-GFP-St monomer, 41 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Mar 18
Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization. Small 21(30):e2502060 (2025)
...Snoj J, Škarabot M, Jerala R
RgGuinier 5.8 nm
Dmax 17.3 nm
VolumePorod 580 nm3

SASDW67 – DRSt - Chimeric protein consisting of DCV, RFP and SpyTag domain

DCV-RFP-St experimental SAS data
MODELLER model
Sample: DCV-RFP-St monomer, 41 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Sep 24
Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization. Small 21(30):e2502060 (2025)
...Snoj J, Škarabot M, Jerala R
RgGuinier 2.8 nm
Dmax 9.2 nm
VolumePorod 60 nm3

SASDW77 – DGSt - Chimeric protein consisting of DCV, GFP and SpyTag domain

DCV-GFP-St experimental SAS data
MODELLER model
Sample: DCV-GFP-St monomer, 41 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Mar 18
Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization. Small 21(30):e2502060 (2025)
...Snoj J, Škarabot M, Jerala R
RgGuinier 2.8 nm
Dmax 9.1 nm
VolumePorod 54 nm3

SASDW87 – DGSt-LucSc - DCV-GFP-SpyTag and NanoLuc-SpyCatcher Conjugate via SpyCatcher-SpyTag Reaction

DCV-GFP-StNanoLuc-Sc experimental SAS data
SREFLEX model
Sample: DCV-GFP-St monomer, 41 kDa synthetic construct protein
NanoLuc-Sc monomer, 30 kDa synthetic construct protein
Buffer: 20 mM Tris, 150 mM NaCL, 3% glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2024 Mar 18
Programmable Protein-DNA Composite Nanostructures: from Nanostructure Construction to Protein-Induced Micro-Scale Material Self-Assembly and Functionalization. Small 21(30):e2502060 (2025)
...Snoj J, Škarabot M, Jerala R
RgGuinier 3.4 nm
Dmax 12.5 nm
VolumePorod 105 nm3