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14 hits found for Wacker

SASDVX2 – Full-length SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

full stem-loop 5 of SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: full stem-loop 5 of SARS-CoV-2 5'genomic end monomer, 48 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
...Wacker A, Mata CP, Carazo JM, Schlundt A, Schwalbe H
RgGuinier 4.3 nm
Dmax 13.8 nm

SASDVY2 – Sub-element stem-loop 5a within the SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

sub-element stem-loop 5a from SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: sub-element stem-loop 5a from SARS-CoV-2 5'genomic end monomer, 11 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
...Wacker A, Mata CP, Carazo JM, Schlundt A, Schwalbe H
RgGuinier 1.9 nm
Dmax 6.0 nm

SASDVC3 – Sub-element stem-loop 5b within the SARS-CoV-2 5'ge element stem-loop 5 (5_SL5)

sub-element stem-loop 5b from SARS-CoV-2 5'genomic end experimental SAS data
PYMOL model
Sample: sub-element stem-loop 5b from SARS-CoV-2 5'genomic end monomer, 8 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 3
Dissecting the Conformational Heterogeneity of Stem-Loop Substructures of the Fifth Element in the 5'-Untranslated Region of SARS-CoV-2. J Am Chem Soc 146(44):30139-30154 (2024)
...Wacker A, Mata CP, Carazo JM, Schlundt A, Schwalbe H
RgGuinier 1.4 nm
Dmax 4.3 nm

SASDVZ3 – SARS-CoV2 RNA pseudoknot at 4 mg/mL

SARS-CoV2 RNA pseudoknot experimental SAS data
SARS-CoV2 RNA pseudoknot Kratky plot
Sample: SARS-CoV2 RNA pseudoknot monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
Optimization of Structure‐Guided Development of Chemical Probes for the Pseudoknot RNA of the Frameshift Element in SARS‐CoV‐2 Angewandte Chemie International Edition (2025)
...Wacker A, Schwalbe H
RgGuinier 2.3 nm
Dmax 7.9 nm
VolumePorod 29 nm3

SASDV24 – SARS-CoV2 RNA pseudoknot at 2 mg/mL

SARS-CoV2 RNA pseudoknot experimental SAS data
SARS-CoV2 RNA pseudoknot Kratky plot
Sample: SARS-CoV2 RNA pseudoknot monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
Optimization of Structure‐Guided Development of Chemical Probes for the Pseudoknot RNA of the Frameshift Element in SARS‐CoV‐2 Angewandte Chemie International Edition (2025)
...Wacker A, Schwalbe H
RgGuinier 2.5 nm
Dmax 8.9 nm
VolumePorod 32 nm3

SASDV34 – SARS-CoV2 RNA pseudoknot at 1 mg/mL

SARS-CoV2 RNA pseudoknot experimental SAS data
SARS-CoV2 RNA pseudoknot Kratky plot
Sample: SARS-CoV2 RNA pseudoknot monomer, 22 kDa Severe acute respiratory … RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Nov 22
Optimization of Structure‐Guided Development of Chemical Probes for the Pseudoknot RNA of the Frameshift Element in SARS‐CoV‐2 Angewandte Chemie International Edition (2025)
...Wacker A, Schwalbe H
RgGuinier 2.5 nm
Dmax 9.5 nm
VolumePorod 32 nm3

SASDSP7 – 5’-terminal stem-loop 1 RNA element of SARS-CoV-2 (5_SL1)

5_SL1 experimental SAS data
DAMMIN model
Sample: 5_SL1 monomer, 9 kDa Severe acute respiratory … RNA
Buffer: 50 mM BisTris, 25 mM NaCl, pH: 6.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Dec 1
The 5'-terminal stem-loop RNA element of SARS-CoV-2 features highly dynamic structural elements that are sensitive to differences in cellular pH. Nucleic Acids Res (2024)
...Wacker A, Faison EM, Duchardt-Ferner E, Richter C, Mathieu D, Bottaro S, Zhang Q, Schwalbe H
RgGuinier 1.7 nm
Dmax 5.5 nm
VolumePorod 16 nm3

SASDRV7 – SARS-CoV-2 5' untranslated region stem-loop 4 RNA (5_SL4) in phosphate buffer

stem-loop 4 from SARS-CoV-2 5'-UTR experimental SAS data
stem-loop 4 from SARS-CoV-2 5'-UTR Kratky plot
Sample: stem-loop 4 from SARS-CoV-2 5'-UTR monomer, 14 kDa SARS coronavirus 2-Wuhan RNA
Buffer: 25 mM potassium phosphate pH 6.5, 150 mM KCl, 2 mM TCEP, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Aug 7
High-resolution structure of stem-loop 4 from the 5'-UTR of SARS-CoV-2 solved by solution state NMR. Nucleic Acids Res (2023)
...Wacker A, Schwalbe H, Wöhnert J, Duchardt-Ferner E
RgGuinier 2.1 nm
Dmax 7.0 nm
VolumePorod 22 nm3

SASDMR9 – Guanidine-II Riboswitch from Escherichia coli 23mer

Guanidin-II riboswitch 23mer experimental SAS data
Guanidin-II riboswitch 23mer Kratky plot
Sample: Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Wacker A, Schwalbe H
RgGuinier 1.5 nm
Dmax 4.8 nm
VolumePorod 14 nm3

SASDMS9 – Guanidine-II Riboswitch from Escherichia coli 23mer with Mg2+

Guanidin-II riboswitch 23mer experimental SAS data
Guanidin-II riboswitch 23mer Kratky plot
Sample: Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Wacker A, Schwalbe H
RgGuinier 1.8 nm
Dmax 5.8 nm
VolumePorod 15 nm3

SASDMT9 – Guanidine-II Riboswitch from Escherichia coli 23mer with Mg2+ and Gdn ligand

Guanidin-II riboswitch 23mer experimental SAS data
Guanidin-II riboswitch 23mer Kratky plot
Sample: Guanidin-II riboswitch 23mer monomer, 7 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, 5 mM GdnCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Wacker A, Schwalbe H
RgGuinier 2.2 nm
Dmax 8.8 nm
VolumePorod 19 nm3

SASDMU9 – Guanidine-II Riboswitch from Escherichia coli 49mer

Guanidin-II riboswitch 49mer experimental SAS data
Guanidin-II riboswitch 49mer Kratky plot
Sample: Guanidin-II riboswitch 49mer dimer, 32 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Wacker A, Schwalbe H
RgGuinier 4.1 nm
Dmax 16.9 nm
VolumePorod 56 nm3

SASDMV9 – Guanidine-II Riboswitch from Escherichia coli 49mer with Mg2+

Guanidin-II riboswitch 49mer experimental SAS data
Guanidin-II riboswitch 49mer Kratky plot
Sample: Guanidin-II riboswitch 49mer dimer, 32 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Wacker A, Schwalbe H
RgGuinier 4.2 nm
Dmax 18.1 nm
VolumePorod 57 nm3

SASDMW9 – Guanidine-II Riboswitch from Escherichia coli 49mer with Mg2+ and Gdn ligand

Guanidin-II riboswitch 49mer experimental SAS data
Guanidin-II riboswitch 49mer Kratky plot
Sample: Guanidin-II riboswitch 49mer hexamer, 95 kDa Escherichia coli RNA
Buffer: 25 mM potassium phosphate, 50 mM KCl, 10 mM Mg2+, 5 mM GdnCl, pH: 6.2
Experiment: SAXS data collected at BM29, ESRF on 2021 Feb 13
Structure and Dynamics of the Guanidine-II Riboswitch from Escherichia coli by NMR Spectroscopy and Small-angle X-ray Scattering (SAXS). Chembiochem (2021)
...Wacker A, Schwalbe H
RgGuinier 8.8 nm
Dmax 33.6 nm
VolumePorod 442 nm3