Small Angle Scattering Biological Data Bank SASBDB


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3 hits found for A-RNA, Bottom-Strand

SASDT65 – Non-structural protein 1 (NS1B, C-terminal) plus double stranded RNA

Non-structural protein 1RNA top strandRNA bottom strand experimental SAS data

Sample:	Non-structural protein 1 monomer, 16 kDa Influenza B virus protein RNA top strand monomer, 5 kDa RNA RNA bottom strand monomer, 5 kDa RNA
Buffer:	40 mM ammonium acetate, pH 5.5, 450 mM NaCl, 5 mM CaCl2, 0.02% NaN3, 50 mM arginine, pH: 5.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 May 18

The NS1 protein of influenza B virus binds 5’-triphosphorylated dsRNA to suppress RIG-I activation and the host antiviral response (2024)
Woltz R, Schweibenz B, Tsutakawa S, Zhao C, Ma L, Shurina B, Hura G, John R, Vorobiev S, Swapna G, Solotchi M, Tainer J, Krug R, Patel S, Montelione G

R_g^Guinier	2.8	nm
D_max	10.3	nm
Volume^Porod	23	nm³

SASDWL5 – I-RNA, an A-to-I hyper-edited 20mer dsRNA

I-RNA, Top-strandI-RNA, Bottom-strand experimental SAS data

I-RNA, Top-strand I-RNA, Bottom-strand Kratky plot

Sample:	I-RNA, Top-strand monomer, 7 kDa RNA ...Bottom-strand monomer, 7 kDa RNA
Buffer:	25 mM sodium phosphate 25 mM sodium chloride, pH: 6.4
Experiment:	SAXS data collected at BM29, ESRF on 2022 Nov 9

Unique conformational dynamics and protein recognition of A-to-I hyper-edited dsRNA. Nucleic Acids Res 53(12) (2025)
Müller-Hermes C, Piomponi V, Hilber S, Asami S, Kreutz C, Bussi G, Sattler M

R_g^Guinier	2.0	nm
D_max	6.9	nm
Volume^Porod	17	nm³

SASDWM5 – A-RNA, non-edited 20mer dsRNA

A-RNA, Top-StrandA-RNA, Bottom-Strand experimental SAS data

A-RNA, Top-Strand A-RNA, Bottom-Strand Kratky plot

Sample:	A-RNA, Top-Strand monomer, 6 kDa RNA A-RNA, Bottom-Strand monomer, 6 kDa RNA
Buffer:	25 mM sodium phosphate 25 mM sodium chloride, pH: 6.4
Experiment:	SAXS data collected at BM29, ESRF on 2022 Nov 9

R_g^Guinier	1.8	nm
D_max	6.2	nm
Volume^Porod	17	nm³

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SASDT65 – Non-structural protein 1 (NS1B, C-terminal) plus double stranded RNA

SASDWL5 – I-RNA, an A-to-I hyper-edited 20mer dsRNA

SASDWM5 – A-RNA, non-edited 20mer dsRNA