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19 hits found for Bifunctional hemolysin/adenylate cyclase

SASDJS3 – ...cyclase toxin CyaA RTX block II-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data
DAMMIF model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 57 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
RgGuinier 4.0 nm
Dmax 13.3 nm
VolumePorod 94 nm3

SASDJT3 – ...cyclase toxin CyaA RTX block III-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data
DAMMIF model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 45 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
RgGuinier 3.4 nm
Dmax 12.1 nm
VolumePorod 85 nm3

SASDJU3 – ...cyclase toxin CyaA RTX block IV-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data
DAMMIF model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 32 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
RgGuinier 2.7 nm
Dmax 8.6 nm
VolumePorod 45 nm3

SASDJ74 – P454 peptide from B.pertussis CyaA toxin complexed with calmodulin

Calmodulin-1Bifunctional hemolysin/adenylate cyclase experimental SAS data
DENSS model
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
Bifunctional hemolysin/adenylate cyclase monomer, 3 kDa Bordetella pertussis protein
Buffer: 20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Sep 24
A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
Voegele A, Sadi M, O'Brien D, Gehan P, Raoux‐Barbot D, Davi M, Hoos S, Brûlé S, Raynal B, Weber P, Mechaly A, Haouz A, Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A
RgGuinier 2.0 nm
Dmax 7.0 nm
VolumePorod 28 nm3

SASDXM8 – ...Bifunctional hemolysin/adenylate cyclase

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Apr 1
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 3.7 nm
Dmax 15.4 nm
VolumePorod 52 nm3

SASDXN8 – ...Bifunctional hemolysin/adenylate cyclase, 1 mM CaCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 1 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Jun 28
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 2.0 nm
Dmax 8.1 nm
VolumePorod 38 nm3

SASDXP8 – ...Bifunctional hemolysin/adenylate cyclase, 10 mM KCl

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Repeats-in-toxin domain Block V of adenylate cyclase toxin Kratky plot
Sample: ...cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 10 mM KCl, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Dec 12
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 3.7 nm
Dmax 15.6 nm
VolumePorod 50 nm3

SASDXQ8 – ...Bifunctional hemolysin/adenylate cyclase, 1 mM MgCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Jun 28
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 3.5 nm
Dmax 14.9 nm
VolumePorod 49 nm3

SASDXR8 – ...Bifunctional hemolysin/adenylate cyclase, 10 mM MgCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 10 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Aug 1
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 3.8 nm
Dmax 16.3 nm
VolumePorod 61 nm3

SASDXS8 – ...Bifunctional hemolysin/adenylate cyclase, 1 mM SrCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 1 mM SrCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Jun 28
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 3.1 nm
Dmax 13.0 nm
VolumePorod 35 nm3

SASDXT8 – ...Bifunctional hemolysin/adenylate cyclase, 2 mM SrCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Repeats-in-toxin domain Block V of adenylate cyclase toxin Kratky plot
Sample: ...cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 2 mM SrCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2025 Jul 22
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 2.7 nm
Dmax 11.5 nm
VolumePorod 34 nm3

SASDXU8 – ...Bifunctional hemolysin/adenylate cyclase, 3 mM SrCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Repeats-in-toxin domain Block V of adenylate cyclase toxin Kratky plot
Sample: ...cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 3 mM SrCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2025 Jul 22
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 2.4 nm
Dmax 11.0 nm
VolumePorod 33 nm3

SASDXV8 – ...Bifunctional hemolysin/adenylate cyclase, 1 mM BaCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 1 mM BaCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Aug 1
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 3.0 nm
Dmax 13.1 nm
VolumePorod 32 nm3

SASDXW8 – ...Bifunctional hemolysin/adenylate cyclase, 3 mM BaCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Repeats-in-toxin domain Block V of adenylate cyclase toxin Kratky plot
Sample: ...cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 3 mM BaCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2025 Jul 22
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 2.5 nm
Dmax 10.9 nm
VolumePorod 34 nm3

SASDXX8 – ...Bifunctional hemolysin/adenylate cyclase, 10 mM BaCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 10 mM BaCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Aug 1
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 2.7 nm
Dmax 11.7 nm
VolumePorod 35 nm3

SASDXY8 – ...Bifunctional hemolysin/adenylate cyclase, 10 mM SrCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
ALPHAFOLD model
Sample: ...cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 10 mM SrCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Aug 1
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 2.1 nm
Dmax 10.1 nm
VolumePorod 36 nm3

SASDXZ8 – ...Bifunctional hemolysin/adenylate cyclase, 3 mM CaCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT , 3 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Dec 12
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 2.0 nm
Dmax 8.8 nm
VolumePorod 38 nm3

SASDCK9 – ...Cyclase Toxin (CyaA)

Bifunctional hemolysin/adenylate cyclase experimental SAS data
ALLOSMOD model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein
Buffer: 20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 19
Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
O'Brien DP, Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A
RgGuinier 2.6 nm
Dmax 9.1 nm
VolumePorod 60 nm3

SASDCL9 – ...Cyclase Toxin (CyaA) in complex with calmodulin

Bifunctional hemolysin/adenylate cyclaseCalmodulin experimental SAS data
BUNCH model
Sample: Bifunctional hemolysin/adenylate cyclase monomer, 39 kDa Bordetella pertussis protein
Calmodulin monomer, 17 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 19
Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
O'Brien DP, Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A
RgGuinier 2.9 nm
Dmax 9.8 nm
VolumePorod 78 nm3