Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase.

Thomsen M, Tuukkanen A Dickerhoff J, Palm GJ, Kratzat H, Svergun DI, Weisz K, Bornscheuer UT, Hinrichs W, Acta Crystallogr D Biol Crystallogr 71(Pt 4):907-17 (2015) Europe PMC

SASDAN6 – Chalcone isomerase, CHI_Δlid construct

Chalcone isomerase deltaLid
MWI(0) 150 kDa
MWexpected 181 kDa
VPorod 270 nm3
log I(s) 2.72×104 2.72×103 2.72×102 2.72×101
Chalcone isomerase deltaLid small angle scattering data  s, nm-1
ln I(s)
Chalcone isomerase deltaLid Guinier plot ln 2.73×104 Rg: 3.6 nm 0 (3.6 nm)-2 s2
(sRg)2I(s)/I(0)
Chalcone isomerase deltaLid Kratky plot 1.104 0 √3 sRg
p(r)
Chalcone isomerase deltaLid pair distance distribution function Rg: 3.5 nm 0 Dmax: 11 nm

Data validation

There are no models related to this curve.

Synchrotron SAXS data from solutions of Chalcone isomerase, CHI_Δlid construct in 50 mM sodium phosphate, pH 6.8 were collected on the EMBL P12 beam line at the PETRA III storage ring (DESY; Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3.1 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.3 and 9.8 mg/ml were measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.

CHI_Δlid deletion mutant is missing amino acid residues His109 - Ala130.

Chalcone isomerase deltaLid (CHI_Δlid)
Mol. type   Protein
Organism   Eubacterium ramulus
Olig. state   Hexamer
Mon. MW   30.2 kDa
 
UniProt   V9P0A9