Staufen-swapping motif is crucial for Staufen dimerization, structure, and Staufen-mediated mRNA decay.

Tripepi A, Shakoor H, Zlobina M, Klumpler T Kubíčková M, Houser J, Klapetek P, Lukavsky PJ, Protein Sci 35(7):e70669 (2026) Europe PMC

SASDWK9 – Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping (ΔSSM) motif

Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488)
MWexperimental 86 kDa
MWexpected 52 kDa
VPorod 105 nm3
log I(s) 2.84×10-1 2.84×10-2 2.84×10-3 2.84×10-4
Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488) small angle scattering data  s, nm-1
ln I(s)
Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488) Guinier plot ln 2.85×10-1 Rg: 5.1 nm 0 (5.1 nm)-2 s2
(sRg)2I(s)/I(0)
Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488) Kratky plot 1.104 0 √3 sRg
p(r)
Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488) pair distance distribution function Rg: 4.7 nm 0 Dmax: 13.8 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping (ΔSSM) motif Rg histogram Rg, nm
Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping (ΔSSM) motif Rg histogram Rg, nm
Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping (ΔSSM) motif Rg histogram Rg, nm
Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping (ΔSSM) motif Rg histogram Rg, nm
Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488) OTHER [STATIC IMAGE] model
Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488) EOM/RANCH model
Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488) EOM/RANCH model
SAXS data from solutions of Human Double-stranded RNA-binding protein Staufen homolog 1 with truncated Staufen-swapping (ΔSSM) motif in 50 mM Tris-HCl, 300 mM NaCl, 3.6 mM β-mercaptoethanol, pH 7 were collected using a Rigaku BioSAXS-1000 instrument at CEITEC (Brno, Czech Republic) equipped with a Pilatus 100K detector at a sample-detector distance of 0.5 m and at a wavelength of λ = 0.154 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). One solute concentration of 0.75 mg/ml was measured at 20°C. Six successive 600 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Double-stranded RNA-binding protein Staufen homolog 1 (81-577 + Δ454-488) (hStau1∆SSM)
Mol. type   Protein
Organism   Homo sapiens
Olig. state   Other
Mon. MW   51.5 kDa
 
UniProt   O95793 (81-577)
Sequence   FASTA