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9 hits found for Genome polyprotein

SASDTR2 – ...polyprotein fragment at pH 5.4

Genome polyprotein experimental SAS data
ALPHAFOLD model
Sample: Genome polyprotein monomer, 13 kDa Turkey avisivirus protein
Buffer: 20 mM citrate pH 5.4, 150 mM NaCl, 2 mM TCEP, 1% glycerol, pH: 5.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 May 2
Solution structure and functional characterization of an avian picornavirus 2AH/NC protein
Anna Wehlin
RgGuinier 1.7 nm
Dmax 7.2 nm
VolumePorod 29 nm3

SASDTS2 – ...polyprotein fragment at pH 6.5

Genome polyprotein experimental SAS data
ALPHAFOLD model
Sample: Genome polyprotein monomer, 13 kDa Turkey avisivirus protein
Buffer: 20mM Citrate buffer pH 6.5, 150mM NaCl, 2mM TCEP, 1% glycerol, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2023 May 15
Solution structure and functional characterization of an avian picornavirus 2AH/NC protein
Anna Wehlin
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 27 nm3

SASDTT2 – ...polyprotein fragment at pH 7.4

Genome polyprotein experimental SAS data
ALPHAFOLD model
Sample: Genome polyprotein monomer, 13 kDa Turkey avisivirus protein
Buffer: 20 mM HEPES, 150 mM NaCl, 2 mM TECP, 1% Glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 May 2
Solution structure and functional characterization of an avian picornavirus 2AH/NC protein
Anna Wehlin
RgGuinier 1.6 nm
Dmax 4.0 nm
VolumePorod 24 nm3

SASDWE4 – The nonstructural 2A protein of the human parechovirus 1 (HPeV1) short form, amino acids 1-129

Genome polyprotein (Protein 2A H-NC; Δ130-150) experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Genome polyprotein (Protein 2A H-NC; Δ130-150) , 15 kDa Human parechovirus 1 … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 5 mM DDT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 28
Structural plasticity of 2A proteins in the Parechovirus family
Zuzanna Pietras
RgGuinier 2.0 nm
Dmax 9.5 nm
VolumePorod 43 nm3

SASDWQ5 – 2A protein from Theiler's murine encephalomyelitis virus (TMEV)

Genome polyprotein experimental SAS data
CORAL model
Sample: Genome polyprotein monomer, 17 kDa Theiler's murine encephalomyelitis … protein
Buffer: 20 mM HEPES pH 7.4, 1 M KCl, 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26
A protein-dependent riboswitch activates ribosomal frameshifting in cardioviruses Molecular Cell (2026)
Betts J, Jeffries C, Passchier T, Kung H, Graham S, Abdelhamid M, Howard J, Craggs T, Graham S, Brierley I, Leake M, Quinn S, Hill C
RgGuinier 1.9 nm
Dmax 7.2 nm
VolumePorod 30 nm3

SASDWS5 – 2A protein from Theiler's murine encephalomyelitis virus (TMEV) bound to stimulatory element RNA

Genome polyproteinStimulatory element RNA experimental SAS data
CORAL model
Sample: Genome polyprotein monomer, 17 kDa Theiler's murine encephalomyelitis … protein
Stimulatory element RNA monomer, 12 kDa synthetic RNA RNA
Buffer: 20 mM HEPES pH 7.4, 400 mM KCl 2% v/v glycerol, 1 mM DTT, pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Apr 26
A protein-dependent riboswitch activates ribosomal frameshifting in cardioviruses Molecular Cell (2026)
Betts J, Jeffries C, Passchier T, Kung H, Graham S, Abdelhamid M, Howard J, Craggs T, Graham S, Brierley I, Leake M, Quinn S, Hill C
RgGuinier 2.2 nm
Dmax 7.5 nm
VolumePorod 46 nm3

SASDUY7 – Aichivirus A 2A protein at neutral pH

Genome polyprotein experimental SAS data
PYMOL model
Sample: Genome polyprotein monomer, 15 kDa Aichi virus (strain … protein
Buffer: 20 mM HEPES,150 mM NaCl, 2 mM TCEP, 1% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 11
Structural and functional repurposing of picornaviral H-box 2A proteins
Marion Pichon
RgGuinier 1.8 nm
Dmax 7.4 nm
VolumePorod 18 nm3

SASDUZ7 – Human Parechovirus 1 2A protein dimer at neutral pH

Genome polyprotein (I918M) experimental SAS data
PYMOL model
Sample: Genome polyprotein (I918M) dimer, 34 kDa Human parechovirus 1 … protein
Buffer: 20 mM HEPES,150 mM NaCl, 2 mM TCEP, 1% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 11
Structural and functional repurposing of picornaviral H-box 2A proteins
Marion Pichon
RgGuinier 2.2 nm
Dmax 8.0 nm
VolumePorod 58 nm3

SASDML9 – Structure of bifunctional protease-helicase NS3/4A domain assembly in solution

Genome polyprotein experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Genome polyprotein dimer, 137 kDa Hepatitis C virus … protein
Buffer: 25 mM Tris, 1 M NaCl, 10% glycerol, 1 mM TCEP, 0.1% β-octyl glucoside, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Dec 12
A macrocyclic HCV NS3/4A protease inhibitor interacts with protease and helicase residues in the complex with its full-length target Proceedings of the National Academy of Sciences 108(52):21052-21056 (2011)
Schiering N, D'Arcy A, Villard F, Simic O, Kamke M, Monnet G, Hassiepen U, Svergun D, Pulfer R, Eder J, Raman P, Bodendorf U
RgGuinier 3.9 nm