| MWexperimental | 62 | kDa |
| MWexpected | 45 | kDa |
| VPorod | 96 | nm3 |
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log I(s)
6.22×101
6.22×100
6.22×10-1
6.22×10-2
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s, nm-1
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Antibody binding geometry and affinity control inhibitory FcγRIIB receptor signaling
Hayden Fisher.SASDUF7 – Deglycosylated Fc gamma receptor IIb (CD32b) extracellular domain in complex with human IgG1 6G08 F(ab')
Low affinity immunoglobulin gamma Fc region receptor II-bHuman IgG1 F(ab') 6G08
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Fits and models
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Synchrotron SAXS data from solutions of deglycosylated Fc gamma receptor IIb (CD32b) extracellular domain in complex with human IgG1 6G08 F(ab') in 50 mM HEPES, 150 mM KCl, pH 7.5 were collected on the BM29 beam line at the ESRF (Grenoble, France) using a Pilatus 1M detector at a sample-detector distance of 2.9 m and at a wavelength of λ = 0.099 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Solute concentrations ranging between 1.3 and 5 mg/ml were measured at 20°C. 10 successive 2 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted. The low angle data collected at lower concentration were merged with the highest concentration high angle data to yield the final composite scattering curve.
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