An NAD+ Phosphorylase Toxin Triggers Mycobacterium tuberculosis Cell Death.

Freire DM, Gutierrez C, Garza-Garcia A, Grabowska AD, Sala AJ, Ariyachaokun K, Panikova T, Beckham KSH, Colom A, Pogenberg V, Cianci M, Tuukkanen A, Boudehen YM, Peixoto A, Botella L, Svergun DI, Schnappinger D, Schneider TR, Genevaux P, de Carvalho LPS, Wilmanns M, Parret AHA, Neyrolles O, Mol Cell (2019) Europe PMC

SASDD33 – Toxin/Antitoxin complex from M. tuberculosis

Mycobacterial cidal toxin
Mycobacterial cidal antitoxin
MWexperimental 154 kDa
MWexpected 197 kDa
VPorod 262 nm3
log I(s) 3.13×104 3.13×103 3.13×102 3.13×101
Mycobacterial cidal toxin Mycobacterial cidal antitoxin small angle scattering data  s, nm-1
ln I(s)
Mycobacterial cidal toxin Mycobacterial cidal antitoxin Guinier plot ln 3.13×104 Rg: 4.1 nm 0 (4.1 nm)-2 s2
(sRg)2I(s)/I(0)
Mycobacterial cidal toxin Mycobacterial cidal antitoxin Kratky plot 1.104 0 √3 sRg
p(r)
Mycobacterial cidal toxin Mycobacterial cidal antitoxin pair distance distribution function Rg: 4.1 nm 0 Dmax: 11.4 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Mycobacterial cidal toxin Mycobacterial cidal antitoxin PDB (PROTEIN DATA BANK) model
log I(s)
 s, nm-1
Mycobacterial cidal toxin Mycobacterial cidal antitoxin DAMMIF model
Synchrotron SAXS data from solutions of the M. tuberculosis suicide toxin/antitoxin complex in 100 mM HEPES, 100 mM NaCl, pH 7.5 were collected on the EMBL bioSAXS P12 beam line at the PETRA III storage ring (DESY, Hamburg, Germany) using a Pilatus 2M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (l(s) vs s, where s = 4πsinθ/λ and 2θ is the scattering angle). One solute concentration of 0.63 mg/ml was measured at 10°C. 20 successive 0.050 second frames were collected. The data were normalized to the intensity of the transmitted beam and radially averaged; the scattering of the solvent-blank was subtracted.

Mycobacterial cidal toxin (MbcT)
Mol. type   Protein
Organism   Mycobacterium tuberculosis
Olig. state   Hexamer
Mon. MW   20.2 kDa
 
UniProt   P9WLP9
Sequence   FASTA
 
Mycobacterial cidal antitoxin (MbcA)
Mol. type   Protein
Organism   Mycobacterium tuberculosis
Olig. state   Hexamer
Mon. MW   12.6 kDa
 
UniProt   P9WLP7
Sequence   FASTA
 
PDB ID   6FKG