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7 hits found for Peroxisomal targeting signal receptor

SASDN33 – Ternary Human Pex5p(C-terminal)-Pex14p(N)-PTS1 Complex (1:1:1 stoichiometry)

Peroxisomal targeting signal 1 receptor (C -terminal)Peroxisomal membrane protein PEX14 (N-terminal)PTS1-BP experimental SAS data
DAMMIN model
Sample: Peroxisomal targeting signal 1 receptor (C -terminal) monomer, 48 kDa Homo sapiens protein
Peroxisomal membrane protein PEX14 (N-terminal) monomer, 7 kDa Homo sapiens protein
PTS1-BP monomer, 14 kDa Homo sapiens protein
Buffer: 50 mM HEPES-KOH (pH 7.5), 100mM KCl, and 20mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Oct 3
Solution structure of human Pex5.Pex14.PTS1 protein complexes obtained by small angle X-ray scattering. J Biol Chem 284(37):25334-42 (2009)
Shiozawa K, Konarev PV, Neufeld C, Wilmanns M, Svergun DI
RgGuinier 2.9 nm
Dmax 9.0 nm
VolumePorod 110 nm3

SASDN43 – Full-length Human Pex5p protein

Peroxisomal targeting signal 1 receptor experimental SAS data
DAMMIN model
Sample: Peroxisomal targeting signal 1 receptor monomer, 71 kDa Homo sapiens protein
Buffer: 50 mM HEPES-KOH (pH 7.5), 100mM KCl, and 20mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Feb 24
Solution structure of human Pex5.Pex14.PTS1 protein complexes obtained by small angle X-ray scattering. J Biol Chem 284(37):25334-42 (2009)
Shiozawa K, Konarev PV, Neufeld C, Wilmanns M, Svergun DI
RgGuinier 5.0 nm
Dmax 20.0 nm
VolumePorod 181 nm3

SASDN53 – Ternary Human Pex5p(full-length)-Pex14p(N)-PTS1 Protein Complex (1:7:1 stoichiometry)

Peroxisomal targeting signal 1 receptorPeroxisomal membrane protein PEX14PTS1-BP experimental SAS data
SASREF model
Sample: Peroxisomal targeting signal 1 receptor monomer, 71 kDa Homo sapiens protein
Peroxisomal membrane protein PEX14 monomer, 7 kDa Homo sapiens protein
PTS1-BP monomer, 13 kDa Homo sapiens protein
Buffer: 50 mM HEPES-KOH (pH 7.5), 100mM KCl, and 20mM TCEP, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Mar 18
Solution structure of human Pex5.Pex14.PTS1 protein complexes obtained by small angle X-ray scattering. J Biol Chem 284(37):25334-42 (2009)
Shiozawa K, Konarev PV, Neufeld C, Wilmanns M, Svergun DI
RgGuinier 6.0 nm
Dmax 20.0 nm
VolumePorod 267 nm3

SASDX84 – Peroxisomal targeting signal receptor Pex5 from P. pastoris

Peroxisomal targeting signal receptor experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Peroxisomal targeting signal receptor monomer, 65 kDa Komagataella pastoris protein
Buffer: 50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 May 15
...peroxisomal receptor Pex5 reveals its essential role in peroxisomal cargo translocation (2025)
Ekal L, Wendscheck D, David Y, Chojnowski G, Jeffries C, Mullapudi E, Schuldiner M, Warscheid B, Zalckvar E, Wilmanns M
RgGuinier 5.0 nm
Dmax 21.4 nm
VolumePorod 131 nm3

SASDXA4 – Peroxisomal biogenesis factor 8 (Pex8) bound to Peroxisomal targeting signal receptor, Pex5 from P. pastoris

Peroxisomal targeting signal receptorPeroxisomal biogenesis factor 8 experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Peroxisomal targeting signal receptor monomer, 65 kDa Komagataella pastoris protein
Peroxisomal biogenesis factor 8 monomer, 78 kDa Komagataella pastoris protein
Buffer: 50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 17
...peroxisomal receptor Pex5 reveals its essential role in peroxisomal cargo translocation (2025)
Ekal L, Wendscheck D, David Y, Chojnowski G, Jeffries C, Mullapudi E, Schuldiner M, Warscheid B, Zalckvar E, Wilmanns M
RgGuinier 5.1 nm
Dmax 23.5 nm
VolumePorod 247 nm3

SASDXB4 – Peroxisomal biogenesis factor 8 (Pex8) bound to the N-terminal domain of Peroxisomal targeting signal receptor, Pex5 (Pex8-Pex5NTD complex from P. pastoris)

Peroxisomal biogenesis factor 8Peroxisomal targeting signal receptor experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Peroxisomal biogenesis factor 8 monomer, 78 kDa Komagataella pastoris protein
Peroxisomal targeting signal receptor monomer, 32 kDa Komagataella pastoris protein
Buffer: 50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 17
...peroxisomal receptor Pex5 reveals its essential role in peroxisomal cargo translocation (2025)
Ekal L, Wendscheck D, David Y, Chojnowski G, Jeffries C, Mullapudi E, Schuldiner M, Warscheid B, Zalckvar E, Wilmanns M
RgGuinier 4.6 nm
Dmax 19.0 nm
VolumePorod 177 nm3

SASDXC4 – Peroxisomal biogenesis factor 8 (Pex8) bound to the C-terminal domain of Peroxisomal targeting signal receptor, Pex5 (Pex8-Pex5CTD complex from P. pastoris)

Peroxisomal biogenesis factor 8Peroxisomal targeting signal receptor experimental SAS data
OTHER [STATIC IMAGE] model
Sample: Peroxisomal biogenesis factor 8 monomer, 78 kDa Komagataella pastoris protein
Peroxisomal targeting signal receptor monomer, 36 kDa Komagataella pastoris protein
Buffer: 50 mM HEPES, 150 mM NaCl, 3% v/v glycerol, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2021 Aug 17
...peroxisomal receptor Pex5 reveals its essential role in peroxisomal cargo translocation (2025)
Ekal L, Wendscheck D, David Y, Chojnowski G, Jeffries C, Mullapudi E, Schuldiner M, Warscheid B, Zalckvar E, Wilmanns M
RgGuinier 4.2 nm
Dmax 14.5 nm
VolumePorod 141 nm3