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14 hits found for Ras GTPase-activating protein 1

SASDPM4 – ...120RasGAP

Ras GTPase-activating protein 1 experimental SAS data
DAMMIF model
Sample: Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein
Buffer: ...1 mM DTT, pH: 8
Experiment: SAXS data collected at ...18ID, Advanced Photon Source (APS), Argonne National Laboratory on ...12
...120RasGAP. Structure (2022)
Stiegler AL, Vish KJ, Boggon TJ
RgGuinier 2.6 nm
Dmax 10.3 nm
VolumePorod 41 nm3

SASDPN4 – ...190RhoGAP Peptide Bound to p120RasGAP's SH2-SH3-SH2 Domains

Ras GTPase-activating protein 1Rho GTPase-activating protein 35 experimental SAS data
DAMMIF model
Sample: Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein
...GTPase-activating protein 35 monomer, 3 kDa Homo sapiens protein
Buffer: ...1 mM DTT, pH: 8
Experiment: SAXS data collected at ...18ID, Advanced Photon Source (APS), Argonne National Laboratory on ...11
...120RasGAP. Structure (2022)
Stiegler AL, Vish KJ, Boggon TJ
RgGuinier 2.4 nm
Dmax 7.9 nm
VolumePorod 41 nm3

SASDWD8 – ...1 (residues 1-466) at pH 7.5, 150 mM NaCl

Ras GTPase-activating protein-binding protein 1 experimental SAS data
Sample: Ras GTPase-activating protein-binding protein 1 dimer, 105 kDa Homo sapiens protein
Buffer: ...150 mM NaCl,, pH: 7.5
Experiment: SAXS data collected at ...1, Diamond Light Source on ...13
...1 conformational dynamics: implications for stress granule formation
RgGuinier 6.6 nm
Dmax 32.4 nm
VolumePorod 221 nm3

SASDWE8 – ...1 (residues 1-466) at pH 7.5, 50 mM NaCl

Ras GTPase-activating protein-binding protein 1 experimental SAS data
DAMMIN model
Sample: Ras GTPase-activating protein-binding protein 1 dimer, 105 kDa Homo sapiens protein
Buffer: ...150 mM NaCl,, pH: 7.5
Experiment: SAXS data collected at ...1, Diamond Light Source on 2023 May 2
...1 conformational dynamics: implications for stress granule formation
RgGuinier 5.8 nm
Dmax 28.7 nm
VolumePorod 218 nm3

SASDWF8 – ...1 (residues 1-466) at pH 6.0, 50 mM NaCl

Ras GTPase-activating protein-binding protein 1 experimental SAS data
Sample: Ras GTPase-activating protein-binding protein 1 dimer, 105 kDa Homo sapiens protein
Buffer: 50 mM MES, 50 mM NaCl,, pH: 6
Experiment: SAXS data collected at ...1, Diamond Light Source on 2023 May 2
...1 conformational dynamics: implications for stress granule formation
RgGuinier 4.9 nm
Dmax 22.5 nm
VolumePorod 163 nm3

SASDWG8 – ...1 (residues 1-140) at pH 7.5, 150 mM NaCl

NTF2L domain of Ras GTPase-activating protein-binding protein 1 experimental SAS data
DAMMIF model
Sample: ...Ras GTPase-activating protein-binding protein 1 dimer, 32 kDa protein
Buffer: ...150 mM NaCl,, pH: 7.5
Experiment: SAXS data collected at ...1, Diamond Light Source on 2023 May 2
...1 conformational dynamics: implications for stress granule formation
RgGuinier 2.0 nm
Dmax 6.5 nm
VolumePorod 40 nm3

SASDWK8 – ...1ΔRGG (residues 1-413) at pH 7.5, 150 mM NaCl

RGG deletion mutant of Ras GTPase-activating protein-binding protein 1 experimental SAS data
DAMMIN model
Sample: ...Ras GTPase-activating protein-binding protein 1 dimer, 94 kDa Homo sapiens protein
Buffer: ...150 mM NaCl,, pH: 7.5
Experiment: SAXS data collected at ...1, Diamond Light Source on 2023 Dec 4
...1 conformational dynamics: implications for stress granule formation
RgGuinier 6.6 nm
Dmax 32.0 nm
VolumePorod 226 nm3

SASDRJ6 – ...Ras GTPase-activating protein 1 (p120RasGAP)

Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) experimental SAS data
Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) Kratky plot
Sample: Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) monomer, 31 kDa Homo sapiens protein
Buffer: ...1 mM DTT, pH: 8
Experiment: SAXS data collected at ...18ID, Advanced Photon Source (APS), Argonne National Laboratory on ...12
...120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 2.5 nm
Dmax 9.8 nm
VolumePorod 42 nm3

SASDRK6 – ...Ras GTPase-activating protein 1 (p120RasGAP) bound to a doubly phosphorylated EphB4 peptide

Ephrin type-B receptor 4Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) experimental SAS data
Ephrin type-B receptor 4 Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) Kratky plot
Sample: Ephrin type-B receptor 4 monomer, 2 kDa Homo sapiens protein
Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) monomer, 31 kDa Homo sapiens protein
Buffer: ...1 mM DTT, pH: 8
Experiment: SAXS data collected at ...18ID, Advanced Photon Source (APS), Argonne National Laboratory on ...12
...120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 2.7 nm
Dmax 10.5 nm
VolumePorod 44 nm3

SASDRL6 – ...Ras GTPase-activating protein 1 (p120RasGAP) bound to a doubly phosphorylated p190RhoGAP peptide

Rho GTPase-activating protein 35Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) experimental SAS data
Rho GTPase-activating protein 35 Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) Kratky plot
Sample: ...GTPase-activating protein 35 monomer, 3 kDa Homo sapiens protein
Ras GTPase-activating protein 1 (C236S, C261S, C372S, C402S) monomer, 31 kDa Homo sapiens protein
Buffer: ...1 mM DTT, pH: 8
Experiment: SAXS data collected at ...18ID, Advanced Photon Source (APS), Argonne National Laboratory on ...12
...120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 2.4 nm
Dmax 7.7 nm
VolumePorod 44 nm3

SASDRE6 – Ras GTPase-activating protein 1 (p120RasGAP, amino acids 174–1047)

Ras GTPase-activating protein 1 experimental SAS data
ALPHAFOLD model
Sample: Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein
Buffer: ...150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at ...18ID, Advanced Photon Source (APS), Argonne National Laboratory on ...1 Nov 4
...120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 4.0 nm
Dmax 14.0 nm
VolumePorod 172 nm3

SASDRF6 – Ras GTPase-activating protein 1 (p120RasGAP, amino acids 174–1047) bound to doubly phosphorylated EphB4 peptide

Ephrin type-B receptor 4Ras GTPase-activating protein 1 experimental SAS data
Ephrin type-B receptor 4 Ras GTPase-activating protein 1 Kratky plot
Sample: Ephrin type-B receptor 4 monomer, 2 kDa Homo sapiens protein
Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein
Buffer: ...150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at ...18ID, Advanced Photon Source (APS), Argonne National Laboratory on ...1 Nov 4
...120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 3.9 nm
Dmax 13.7 nm
VolumePorod 176 nm3

SASDRG6 – Ras GTPase-activating protein 1 (p120RasGAP, amino acids 174–1047) bound to doubly phosphorylated p190RhoGAP peptide

Rho GTPase-activating protein 35Ras GTPase-activating protein 1 experimental SAS data
Rho GTPase-activating protein 35 Ras GTPase-activating protein 1 Kratky plot
Sample: ...GTPase-activating protein 35 monomer, 3 kDa Homo sapiens protein
Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein
Buffer: ...150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at ...18ID, Advanced Photon Source (APS), Argonne National Laboratory on ...1 Nov 4
...120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 4.0 nm
Dmax 15.1 nm
VolumePorod 194 nm3

SASDRH6 – Ras GTPase-activating protein 1 (p120RasGAP, amino acids 174–1047) bound to a doubly phosphorylated Dok1 peptide

Ras GTPase-activating protein 1Docking protein 1 experimental SAS data
Ras GTPase-activating protein 1 Docking protein 1 Kratky plot
Sample: Ras GTPase-activating protein 1 monomer, 101 kDa Homo sapiens protein
...protein 1 monomer, 3 kDa Homo sapiens protein
Buffer: ...150 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at ...18ID, Advanced Photon Source (APS), Argonne National Laboratory on ...1 Nov 4
...120RasGAP interactions with doubly phosphorylated partners EphB4, p190RhoGAP and Dok1 Journal of Biological Chemistry :105098 (2023)
Vish K, Stiegler A, Boggon T
RgGuinier 4.0 nm
Dmax 14.6 nm
VolumePorod 196 nm3