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30 hits found for adenylate cyclase

SASDL62 – ...adenylate cyclase toxin CyaA - construct RTX-1 (amino acids 1132-1294 and 1562-1681 of CyaA)

hybrid RTX-1 construct (amino acids 1132-1294 and 1562-1681 of CyaA) experimental SAS data
DAMFILT model
Sample: hybrid RTX-1 construct (amino acids 1132-1294 and 1562-1681 of CyaA) monomer, 30 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 1
...adenylate cyclase toxin. J Biol Chem :100833 (2021)
Espinosa-Vinals CA, Masin J, Holubova J, Stanek O, Jurnecka D, Osicka R, Sebo P, Bumba L
RgGuinier 2.4 nm
Dmax 8.2 nm
VolumePorod 41 nm3

SASDL72 – ...adenylate cyclase toxin CyaA - construct RTX-2 (amino acids 1132-1303 and 1562-1681 of CyaA)

hybrid RTX-2 construct (amino acids 1132-1303 and 1562-1681 of CyaA) experimental SAS data
DAMMIF model
Sample: hybrid RTX-2 construct (amino acids 1132-1303 and 1562-1681 of CyaA) monomer, 31 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Dec 1
...adenylate cyclase toxin. J Biol Chem :100833 (2021)
Espinosa-Vinals CA, Masin J, Holubova J, Stanek O, Jurnecka D, Osicka R, Sebo P, Bumba L
RgGuinier 2.3 nm
Dmax 8.0 nm
VolumePorod 37 nm3

SASDJS3 – Adenylate cyclase toxin CyaA RTX block II-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data
DAMMIF model
Sample: ...adenylate cyclase monomer, 57 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
RgGuinier 4.0 nm
Dmax 13.3 nm
VolumePorod 94 nm3

SASDJT3 – Adenylate cyclase toxin CyaA RTX block III-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data
DAMMIF model
Sample: ...adenylate cyclase monomer, 45 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
RgGuinier 3.4 nm
Dmax 12.1 nm
VolumePorod 85 nm3

SASDJU3 – Adenylate cyclase toxin CyaA RTX block IV-V

Bifunctional hemolysin/adenylate cyclase experimental SAS data
DAMMIF model
Sample: ...adenylate cyclase monomer, 32 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl, 150 mM NaCl, 10 mM CaCl₂, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Continuous Assembly of β-Roll Structures Is Implicated in the Type I-Dependent Secretion of Large Repeat-in-Toxins (RTX) Proteins. J Mol Biol (2020)
Motlova L, Klimova N, Fiser R, Sebo P, Bumba L
RgGuinier 2.7 nm
Dmax 8.6 nm
VolumePorod 45 nm3

SASDB74 – CyaA Block I-V

Adenylate cyclase toxin Block I-V experimental SAS data
DAMMIF model
Sample: Adenylate cyclase toxin Block I-V monomer, 70 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl 150 mM NaCl 10 mM CaCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Calcium-Driven Folding of RTX Domain β-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts. Mol Cell 62(1):47-62 (2016)
Bumba L, Masin J, Macek P, Wald T, Motlova L, Bibova I, Klimova N, Bednarova L, Veverka V, Kachala M, Svergun DI, Barinka C, Sebo P
RgGuinier 5.6 nm
Dmax 17.2 nm
VolumePorod 275 nm3

SASDJ74 – P454 peptide from B.pertussis CyaA toxin complexed with calmodulin

Calmodulin-1Bifunctional hemolysin/adenylate cyclase experimental SAS data
DENSS model
Sample: Calmodulin-1 monomer, 17 kDa Homo sapiens protein
...adenylate cyclase monomer, 3 kDa Bordetella pertussis protein
Buffer: 20 mM HEPES, 150 mM NaCl, 4 mM CaCl₂, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Sep 24
A High‐Affinity Calmodulin‐Binding Site in the CyaA Toxin Translocation Domain is Essential for Invasion of Eukaryotic Cells Advanced Science :2003630 (2021)
Voegele A, Sadi M, O'Brien D, Gehan P, Raoux‐Barbot D, Davi M, Hoos S, Brûlé S, Raynal B, Weber P, Mechaly A, Haouz A, Rodriguez N, Vachette P, Durand D, Brier S, Ladant D, Chenal A
RgGuinier 2.0 nm
Dmax 7.0 nm
VolumePorod 28 nm3

SASDB84 – CyaA Block V

Adenylate cyclase toxin Block V experimental SAS data
DAMMIF model
Sample: Adenylate cyclase toxin Block V monomer, 16 kDa Bordetella pertussis protein
Buffer: 10 mM Tris HCl 150 mM NaCl 10 mM CaCl2, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 31
Calcium-Driven Folding of RTX Domain β-Rolls Ratchets Translocation of RTX Proteins through Type I Secretion Ducts. Mol Cell 62(1):47-62 (2016)
Bumba L, Masin J, Macek P, Wald T, Motlova L, Bibova I, Klimova N, Bednarova L, Veverka V, Kachala M, Svergun DI, Barinka C, Sebo P
RgGuinier 1.8 nm
Dmax 5.9 nm
VolumePorod 24 nm3

SASDGM5 – ...Adenylate Cyclase Toxin (CyaA)

RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) experimental SAS data
OTHER model
Sample: ...Adenylate Cyclase Toxin (CyaA) monomer, 73 kDa Bordetella pertussis protein
Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM DTT, 4 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 May 31
Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion. Sci Rep 5:14223 (2015)
O'Brien DP, Hernandez B, Durand D, Hourdel V, Sotomayor-Pérez AC, Vachette P, Ghomi M, Chamot-Rooke J, Ladant D, Brier S, Chenal A
RgGuinier 4.4 nm
Dmax 15.5 nm
VolumePorod 89 nm3

SASDGN5 – ...Adenylate Cyclase Toxin (CyaA)

RD domain of B. Pertussis Adenylate Cyclase Toxin (CyaA) experimental SAS data
OTHER model
Sample: ...Adenylate Cyclase Toxin (CyaA) monomer, 73 kDa Bordetella pertussis protein
Buffer: 20 mM Hepes, 150 mM NaCl, 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2012 May 31
Structural models of intrinsically disordered and calcium-bound folded states of a protein adapted for secretion. Sci Rep 5:14223 (2015)
O'Brien DP, Hernandez B, Durand D, Hourdel V, Sotomayor-Pérez AC, Vachette P, Ghomi M, Chamot-Rooke J, Ladant D, Brier S, Chenal A
RgGuinier 8.3 nm
Dmax 33.0 nm

SASDU78 – Trypanosoma brucei ESAG4 membrane-proximal Venus Fly Trap domain 2 (VFT2)

adenylate cyclase experimental SAS data
ALPHAFOLD model
Sample: adenylate cyclase monomer, 43 kDa Trypanosoma brucei protein
Buffer: 50 mM Tris-HCl, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2023 Sep 23
...adenylate cyclase from Trypanosoma brucei. Mol Biochem Parasitol 260:111653 (2024)
Alves DO, Geens R, da Silva Arruda HR, Jennen L, Corthaut S, Wuyts E, de Andrade GC, Prosdocimi F, Cordeiro Y, Pires JR, Vieira LR, de Oliveira GAP, Sterckx YG, Salmon D
RgGuinier 2.3 nm
Dmax 7.6 nm
VolumePorod 76 nm3

SASDXM8 – ...adenylate cyclase

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Apr 1
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 3.7 nm
Dmax 15.4 nm
VolumePorod 52 nm3

SASDXN8 – ...adenylate cyclase, 1 mM CaCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 1 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Jun 28
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 2.0 nm
Dmax 8.1 nm
VolumePorod 38 nm3

SASDXP8 – ...adenylate cyclase, 10 mM KCl

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Repeats-in-toxin domain Block V of adenylate cyclase toxin Kratky plot
Sample: ...adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 10 mM KCl, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Dec 12
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 3.7 nm
Dmax 15.6 nm
VolumePorod 50 nm3

SASDXQ8 – ...adenylate cyclase, 1 mM MgCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 1 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Jun 28
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 3.5 nm
Dmax 14.9 nm
VolumePorod 49 nm3

SASDXR8 – ...adenylate cyclase, 10 mM MgCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 10 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Aug 1
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 3.8 nm
Dmax 16.3 nm
VolumePorod 61 nm3

SASDXS8 – ...adenylate cyclase, 1 mM SrCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 1 mM SrCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Jun 28
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 3.1 nm
Dmax 13.0 nm
VolumePorod 35 nm3

SASDXT8 – ...adenylate cyclase, 2 mM SrCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Repeats-in-toxin domain Block V of adenylate cyclase toxin Kratky plot
Sample: ...adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 2 mM SrCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2025 Jul 22
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 2.7 nm
Dmax 11.5 nm
VolumePorod 34 nm3

SASDXU8 – ...adenylate cyclase, 3 mM SrCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Repeats-in-toxin domain Block V of adenylate cyclase toxin Kratky plot
Sample: ...adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 3 mM SrCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2025 Jul 22
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 2.4 nm
Dmax 11.0 nm
VolumePorod 33 nm3

SASDXV8 – ...adenylate cyclase, 1 mM BaCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 1 mM BaCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Aug 1
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 3.0 nm
Dmax 13.1 nm
VolumePorod 32 nm3

SASDXW8 – ...adenylate cyclase, 3 mM BaCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Repeats-in-toxin domain Block V of adenylate cyclase toxin Kratky plot
Sample: ...adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 3 mM BaCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2025 Jul 22
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 2.5 nm
Dmax 10.9 nm
VolumePorod 34 nm3

SASDXX8 – ...adenylate cyclase, 10 mM BaCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 10 mM BaCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Aug 1
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 2.7 nm
Dmax 11.7 nm
VolumePorod 35 nm3

SASDXY8 – ...adenylate cyclase, 10 mM SrCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
ALPHAFOLD model
Sample: ...adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT, 10 mM SrCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Aug 1
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 2.1 nm
Dmax 10.1 nm
VolumePorod 36 nm3

SASDXZ8 – ...adenylate cyclase, 3 mM CaCl2

Repeats-in-toxin domain Block V of adenylate cyclase toxin experimental SAS data
Sample: ...adenylate cyclase toxin monomer, 19 kDa Bordetella pertussis protein
Buffer: 50 mM Tris, 5 mM DTT , 3 mM CaCl2, pH: 7.5
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2024 Dec 12
Ion-selective conformational stabilization of a disordered repeats-in-toxin protein domain. Biophys J (2025)
Gudinas AP, Shambharkar GM, Chang MP, Fernández D, Matsui T, Mai DJ
RgGuinier 2.0 nm
Dmax 8.8 nm
VolumePorod 38 nm3

SASDCK9 – ...Adenylate Cyclase Toxin (CyaA)

Bifunctional hemolysin/adenylate cyclase experimental SAS data
ALLOSMOD model
Sample: ...adenylate cyclase monomer, 39 kDa Bordetella pertussis protein
Buffer: 20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 19
Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
O'Brien DP, Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A
RgGuinier 2.6 nm
Dmax 9.1 nm
VolumePorod 60 nm3

SASDCL9 – ...Adenylate Cyclase Toxin (CyaA) in complex with calmodulin

Bifunctional hemolysin/adenylate cyclaseCalmodulin experimental SAS data
BUNCH model
Sample: ...adenylate cyclase monomer, 39 kDa Bordetella pertussis protein
Calmodulin monomer, 17 kDa Homo sapiens protein
Buffer: 20 mM Hepes, 150 mM NaCl, 4 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Jun 19
Calmodulin fishing with a structurally disordered bait triggers CyaA catalysis. PLoS Biol 15(12):e2004486 (2017)
O'Brien DP, Durand D, Voegele A, Hourdel V, Davi M, Chamot-Rooke J, Vachette P, Brier S, Ladant D, Chenal A
RgGuinier 2.9 nm
Dmax 9.8 nm
VolumePorod 78 nm3

SASDPP3 – ...adenylate cyclase toxin (CyaA)

Double-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 experimental SAS data
RTX719. Double-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA) Rg histogram
Sample: Double-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 monomer, 78 kDa Bordetella pertussis protein
Buffer: 50 mM NaCl, 3 mM CaCl2, 20 mM Tris, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Feb 15
...adenylate cyclase toxin with cell membrane. J Biol Chem :110392 (2025)
Espinosa-Vinals C, Stransky J, Osicka R, Osickova A, Jurnecka D, Sebo P, Bumba L
RgGuinier 3.4 nm
Dmax 14.5 nm
VolumePorod 67 nm3

SASDPQ3 – ...adenylate cyclase toxin (CyaA)

Mono-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 experimental SAS data
RTX719-K860R. Mono-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA) Rg histogram
Sample: Mono-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 monomer, 78 kDa Bordetella pertussis protein
Buffer: 50 mM NaCl, 3 mM CaCl2, 20 mM Tris, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Feb 16
...adenylate cyclase toxin with cell membrane. J Biol Chem :110392 (2025)
Espinosa-Vinals C, Stransky J, Osicka R, Osickova A, Jurnecka D, Sebo P, Bumba L
RgGuinier 5.0 nm
Dmax 25.8 nm
VolumePorod 178 nm3

SASDPR3 – ...adenylate cyclase toxin (CyaA)

Mono-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 experimental SAS data
RTX719-K983R. Mono-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA) Rg histogram
Sample: Mono-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 monomer, 78 kDa Bordetella pertussis protein
Buffer: 50 mM NaCl, 3 mM CaCl2, 20 mM Tris, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Feb 17
...adenylate cyclase toxin with cell membrane. J Biol Chem :110392 (2025)
Espinosa-Vinals C, Stransky J, Osicka R, Osickova A, Jurnecka D, Sebo P, Bumba L
RgGuinier 5.3 nm
Dmax 26.3 nm
VolumePorod 187 nm3

SASDPS3 – ...adenylate cyclase toxin (CyaA)

Non-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 experimental SAS data
proRTX719. Non-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA) Rg histogram
Sample: Non-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 monomer, 78 kDa Bordetella pertussis protein
Buffer: 50 mM NaCl, 3 mM CaCl2, 20 mM Tris, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpoint 2.0, Institute of Biotechnology, Czech Academy of Sciences/Centre of Molecular Structure on 2022 Feb 22
...adenylate cyclase toxin with cell membrane. J Biol Chem :110392 (2025)
Espinosa-Vinals C, Stransky J, Osicka R, Osickova A, Jurnecka D, Sebo P, Bumba L
RgGuinier 4.2 nm
Dmax 17.8 nm
VolumePorod 133 nm3