Small Angle Scattering Biological Data Bank SASBDB


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6 hits found for Endo-1,4-beta-xylanase

SASDUD4 – Xylanase Updated Consensus SAXS Data

Endo-1,4-beta-xylanase experimental SAS data

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4

Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
Trewhella J, Vachette P, Larsen A

R_g^Guinier	1.6	nm
D_max	5.2	nm

SASDPS4 – Consensus SAXS Profile - Xylanase

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

A round-robin approach provides a detailed assessment of biomolecular small-angle scattering data reproducibility and yields consensus curves for benchmarking Acta Crystallographica Section D Structural Biology 78(11) (2022)
Trewhella J, Vachette P, Bierma J, Blanchet C, Brookes E, Chakravarthy S, Chatzimagas L, Cleveland T, Cowieson N, Crossett B, Duff A, Franke D, Gabel F, Gillilan R, Graewert M, Grishaev A, Guss J, Ham...

R_g^Guinier	1.6	nm
D_max	5.1	nm
Volume^Porod	27	nm³

SASDPW4 – Consensus SANS Profile - Xylanase in 100% v/v D2O buffer

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 6

R_g^Guinier	1.5	nm
D_max	4.4	nm

SASDP35 – Consensus SANS Profile - Xylanase in H2O buffer

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment:	SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2022 Jun 22

R_g^Guinier	1.6	nm
D_max	4.3	nm

SASDPM7 – Xylanase SEC-SAXS data (EMBL-P12 bioSAXS beam line at DESY)

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM HEPES, 150 mM KCl, 3% glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Sep 16

Small-Angle X-ray Scattering data (benchmarking/consensus): EMBL-P12 SAXS beam line, DESY
Clement Blanchet, Melissa Graewert, Cy M Jeffries, Dmitri Svergun

R_g^Guinier	1.6	nm
D_max	4.8	nm
Volume^Porod	25	nm³

SASDPN7 – Xylanase SEC-WAXS data (EMBL-P12 bioSAXS beam line at DESY)

Sample:	Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer:	50 mM Tris, 100 mM NaCl, 1% v/v glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jul 8