Salmonella enterica BcfH is a trimeric thioredoxin-like bifunctional enzyme with both thiol oxidase and disulfide isomerase activities.

Subedi P, Paxman JJ, Wang G, Hor L, Hong Y, Verderosa AD, Whitten AE, Panjikar S, Santos-Martin CF, Martin JL, Totsika M, Heras B, Antioxid Redox Signal (2021) Europe PMC

SASDJL7 – Oxidised fimbrial BcfH protein

Hypothetical exported protein
MWI(0) 72 kDa
MWexpected 83 kDa
VPorod 93 nm3
log I(s) 1.68×10-2 1.68×10-3 1.68×10-4 1.68×10-5
Hypothetical exported protein small angle scattering data  s, nm-1
ln I(s)
Hypothetical exported protein Guinier plot ln 1.69×10-2 Rg: 3.2 nm 0 (3.2 nm)-2 s2
(sRg)2I(s)/I(0)
Hypothetical exported protein Kratky plot 1.104 0 √3 sRg
p(r)
Hypothetical exported protein pair distance distribution function Rg: 3.2 nm 0 Dmax: 11.5 nm

Data validation

Fits and models

log I(s)
 s, nm-1
Hypothetical exported protein CORAL model
log I(s)
 s, nm-1
Hypothetical exported protein DAMFILT model
X-ray synchrotron radiation scattering data from BcfH in 25 mM HEPES 150 mM NaCl, pH 7.5 were collected on the SAXS/WAXS beam line of the Australian Synchrotron (Melbourne, Australia) using a 2D Photon counting Pilatus 1M-W pixel detector (s = 4π sin θ/λ, where 2θ is the scattering angle). Thirty five successive 1 second frames were collected at 0.3 mg/ml. The data were normalized to the intensity of the transmitted beam and radially averaged and the scattering of the solvent-blank was subtracted. The models and corresponding fits include those derived from dummy-atom modelling using DAMMIN (16 models averaged and filtered using damaver), and CORAL (the best of 16 models) are provided here.

Hypothetical exported protein (BcfH)
Mol. type   Protein
Organism   Salmonella typhimurium (strain SL1344)
Olig. state   Trimer
Mon. MW   27.6 kDa
 
UniProt   A0A0H3N7J9 (28-281)
Sequence   FASTA
 
PDB ID   7JVE