Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach

Lahey-Rudolph J, Schönherr R, Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L, Journal of Applied Crystallography 53(5) (2020) DOI

SASDH46 – High Five insect cell culture infected with mock recombinant baculovirus (rBV)

mock recombinant baculovirus
MWexperimental 0 kDa
MWexpected 0 kDa
log I(s) 3.50×105 3.50×104 3.50×103 3.50×102
mock recombinant baculovirus small angle scattering data  s, nm-1
(sRg)2I(s)/I(0)
mock recombinant baculovirus Kratky plot 1.104 0 √3 sRg

Data validation

There are no models related to this curve.

Synchrotron SAXS data from High Five cell cultures infected with mock recombinant baculovirus (rBV) were collected on the EMBL P12 beam line at PETRA III (DESY; Hamburg, Germany) using a Pilatus 6M detector at a sample-detector distance of 3 m and at a wavelength of λ = 0.124 nm (I(s) vs s, where s = 4πsinθ/λ, and 2θ is the scattering angle). Data were collected at 7°C using a fixed sample position (no sample flow) in a 1.8 mm capillary. 40 successive 0.045 second frames were collected; the individual 2D images were summed (1.8 s total exposure) and then normalized to the intensity of the transmitted beam and radially averaged. The scattering of the solvent-blank was subtracted.

The High Five cell cultures were suspended in TBS (20 mM Tris, 150 mM NaCl), pH 7, that was used for solvent-background scattering subtraction. Data validation metrics do not apply for this entry (Rg, I(0), MW, etc).

mock recombinant baculovirus (mock-rBV)
Mol. type   Other
Organism   unidentified baculovirus
Olig. state   Unknown
Mon. MW   0.0 kDa
Chemical formula