SASBDB entries for UniProt ID:

SASDGU9 – Nucleolar RNA helicase 2 (DDX21-FL)

UniProt ID: Q9NR30 (1-783) Nucleolar RNA helicase 2
Nucleolar RNA helicase 2 experimental SAS data
DAMMIF model
Sample: Nucleolar RNA helicase 2 dimer, 182 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Nov 25
The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity iScience 23(12):101811 (2020)
Marcaida M, Kauzlaric A, Duperrex A, Sülzle J, Moncrieffe M, Adebajo D, Manley S, Trono D, Dal Peraro M
RgGuinier 7.0 nm
Dmax 31.9 nm
VolumePorod 681 nm3

SASDGV9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-783

UniProt ID: Q9NR30 (186-783) Nucleolar RNA helicase 2 fragment 186-783
Nucleolar RNA helicase 2 fragment 186-783 experimental SAS data
DAMMIF model
Sample: Nucleolar RNA helicase 2 fragment 186-783 dimer, 138 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 4
The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity iScience 23(12):101811 (2020)
Marcaida M, Kauzlaric A, Duperrex A, Sülzle J, Moncrieffe M, Adebajo D, Manley S, Trono D, Dal Peraro M
RgGuinier 4.8 nm
Dmax 17.6 nm
VolumePorod 297 nm3

SASDGW9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-710

UniProt ID: Q9NR30 (186-710) Nucleolar RNA helicase 2 fragment 186-710
Nucleolar RNA helicase 2 fragment 186-710 experimental SAS data
Nucleolar RNA helicase 2 (DDX21) fragment 186-710 Rg histogram
Sample: Nucleolar RNA helicase 2 fragment 186-710 dimer, 121 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 4
The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity iScience 23(12):101811 (2020)
Marcaida M, Kauzlaric A, Duperrex A, Sülzle J, Moncrieffe M, Adebajo D, Manley S, Trono D, Dal Peraro M
RgGuinier 4.6 nm
Dmax 17.7 nm
VolumePorod 238 nm3

SASDGX9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-620

UniProt ID: Q9NR30 (186-620) Nucleolar RNA helicase 2 fragment 186-620
Nucleolar RNA helicase 2 fragment 186-620 experimental SAS data
SWISSMODEL model
Sample: Nucleolar RNA helicase 2 fragment 186-620 dimer, 98 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Mar 4
The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity iScience 23(12):101811 (2020)
Marcaida M, Kauzlaric A, Duperrex A, Sülzle J, Moncrieffe M, Adebajo D, Manley S, Trono D, Dal Peraro M
RgGuinier 4.4 nm
Dmax 16.9 nm
VolumePorod 150 nm3

SASDGY9 – Nucleolar RNA helicase 2 (DDX21) fragment 186-783 monomeric mutant

UniProt ID: Q9NR30 (186-783) Nucleolar RNA helicase 2
Nucleolar RNA helicase 2 experimental SAS data
Nucleolar RNA helicase 2 Kratky plot
Sample: Nucleolar RNA helicase 2 monomer, 69 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2018 Nov 3
The Human RNA Helicase DDX21 Presents a Dimerization Interface Necessary for Helicase Activity iScience 23(12):101811 (2020)
Marcaida M, Kauzlaric A, Duperrex A, Sülzle J, Moncrieffe M, Adebajo D, Manley S, Trono D, Dal Peraro M
RgGuinier 4.1 nm
Dmax 17.7 nm
VolumePorod 111 nm3

SASDGZ9 – Protein Disulfide Isomerase (PDI) purified from bovine liver

UniProt ID: P05307 (1-510) Protein disulfide-isomerase
Protein disulfide-isomerase experimental SAS data
DAMMIF model
Sample: Protein disulfide-isomerase monomer, 57 kDa Bos taurus protein
Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4
Insights on the dynamic behavior of protein disulfide isomerase in the solution environment through the SAXS technique In Silico Pharmacology 12(1) (2024)
Sanyasi C, Balakrishnan S, Chinnasamy T, Venugopalan N, Kandavelu P, Batra-Safferling R, Muthuvel S
RgGuinier 3.0 nm
Dmax 9.8 nm
VolumePorod 98 nm3

SASDH22 – Protein Disulfide Isomerase (PDI) in complex with Bovine Pancreatic Trypsin Inhibitor (BPTI)

UniProt ID: P05307 (1-510) Protein disulfide-isomerase

UniProt ID: P00974 (36-93) Pancreatic trypsin inhibitor
Protein disulfide-isomerasePancreatic trypsin inhibitor experimental SAS data
DAMMIF model
Sample: Protein disulfide-isomerase monomer, 57 kDa Bos taurus protein
Pancreatic trypsin inhibitor monomer, 7 kDa Bos taurus protein
Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4
Insights on the dynamic behavior of protein disulfide isomerase in the solution environment through the SAXS technique In Silico Pharmacology 12(1) (2024)
Sanyasi C, Balakrishnan S, Chinnasamy T, Venugopalan N, Kandavelu P, Batra-Safferling R, Muthuvel S
RgGuinier 3.2 nm
Dmax 8.9 nm
VolumePorod 88 nm3

SASDH32 – Protein Disulfide Isomerase (PDI) in Complex with Ribonuclease-A (RNase)

UniProt ID: P05307 (1-510) Protein disulfide-isomerase

UniProt ID: P61823 (27-150) Ribonuclease pancreatic
Protein disulfide-isomeraseRibonuclease pancreatic experimental SAS data
DAMMIF model
Sample: Protein disulfide-isomerase monomer, 57 kDa Bos taurus protein
Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4
Insights on the dynamic behavior of protein disulfide isomerase in the solution environment through the SAXS technique In Silico Pharmacology 12(1) (2024)
Sanyasi C, Balakrishnan S, Chinnasamy T, Venugopalan N, Kandavelu P, Batra-Safferling R, Muthuvel S
RgGuinier 3.0 nm
Dmax 9.1 nm
VolumePorod 85 nm3

SASDH42 – Bovine Pancreatic Trypsin Inhibitor (BPTI) in solution environment at pH 7.5

UniProt ID: P00974 (36-93) Pancreatic trypsin inhibitor
Pancreatic trypsin inhibitor experimental SAS data
DAMMIF model
Sample: Pancreatic trypsin inhibitor dimer, 13 kDa Bos taurus protein
Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4
Insights on the dynamic behavior of protein disulfide isomerase in the solution environment through the SAXS technique In Silico Pharmacology 12(1) (2024)
Sanyasi C, Balakrishnan S, Chinnasamy T, Venugopalan N, Kandavelu P, Batra-Safferling R, Muthuvel S
RgGuinier 1.4 nm
Dmax 4.6 nm
VolumePorod 6 nm3

SASDH52 – Ribonuclease A (Type III-A) in a solution environment at pH 7.5

UniProt ID: P61823 (27-150) Ribonuclease pancreatic
Ribonuclease pancreatic experimental SAS data
DAMMIF model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris-HCL, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2015 Nov 4
Insights on the dynamic behavior of protein disulfide isomerase in the solution environment through the SAXS technique In Silico Pharmacology 12(1) (2024)
Sanyasi C, Balakrishnan S, Chinnasamy T, Venugopalan N, Kandavelu P, Batra-Safferling R, Muthuvel S
RgGuinier 1.9 nm
Dmax 7.2 nm
VolumePorod 20 nm3