SASBDB entries for UniProt ID:

SASDHA6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells

UniProt ID: P87252 (1-176) Woronin body major protein
Woronin body major protein experimental SAS data
Woronin body major protein Kratky plot
Sample: Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21
Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
Lahey-Rudolph J, Schönherr R, Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDHB6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within Sf9 insect cells

UniProt ID: P87252 (1-176) Woronin body major protein
Woronin body major protein experimental SAS data
Woronin body major protein Kratky plot
Sample: Woronin body major protein, 19 kDa Neurospora crassa protein
Buffer: TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21
Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
Lahey-Rudolph J, Schönherr R, Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDHG6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 36.3 Gy X-ray dose (J/kg)

UniProt ID: P04067 (47-313) Endo-beta-N-acetylglucosaminidase H
Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 3.0 nm
VolumePorod 69 nm3

SASDHH6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 72.6 Gy X-ray dose (J/kg)

UniProt ID: P04067 (47-313) Endo-beta-N-acetylglucosaminidase H
Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 3.1 nm
VolumePorod 68 nm3

SASDHJ6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 108.9 Gy X-ray dose (J/kg)

UniProt ID: P04067 (47-313) Endo-beta-N-acetylglucosaminidase H
Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 3.0 nm
VolumePorod 67 nm3

SASDHK6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 145.2 Gy X-ray dose (J/kg)

UniProt ID: P04067 (47-313) Endo-beta-N-acetylglucosaminidase H
Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 3.0 nm
VolumePorod 66 nm3

SASDHL6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 181.5 Gy X-ray dose (J/kg)

UniProt ID: P04067 (47-313) Endo-beta-N-acetylglucosaminidase H
Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 2.9 nm
VolumePorod 65 nm3

SASDHM6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 217.8 Gy X-ray dose (J/kg)

UniProt ID: P04067 (47-313) Endo-beta-N-acetylglucosaminidase H
Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 2.9 nm
VolumePorod 64 nm3

SASDHN6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 254.1 Gy X-ray dose (J/kg)

UniProt ID: P04067 (47-313) Endo-beta-N-acetylglucosaminidase H
Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 2.9 nm
VolumePorod 64 nm3

SASDHP6 – N-terminal engineered, disulfide-containing endo-beta-N-acetylglucosaminidase H at 290.4 Gy X-ray dose (J/kg)

UniProt ID: P04067 (47-313) Endo-beta-N-acetylglucosaminidase H
Endo-beta-N-acetylglucosaminidase H experimental SAS data
Endo-beta-N-acetylglucosaminidase H Kratky plot
Sample: Endo-beta-N-acetylglucosaminidase H dimer, 61 kDa Streptomyces plicatus protein
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM EDTA, pH: 7.5
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 Nov 4
SAXS studies of X-ray induced disulfide bond damage: Engineering high-resolution insight from a low-resolution technique PLOS ONE 15(11):e0239702 (2020)
Stachowski T, Snell M, Snell E, Boggon T
RgGuinier 2.9 nm
VolumePorod 63 nm3