SASBDB entries for UniProt ID:

SASDJM9 – Saposin-A plus MNA C12 (1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol)

UniProt ID: None (60-140) Prosaposin (Saposin-A)

UniProt ID: None (None-None) 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol
Prosaposin (Saposin-A)1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol experimental SAS data
Prosaposin (Saposin-A) 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol Kratky plot
Sample: Prosaposin (Saposin-A) dimer, 19 kDa Homo sapiens protein
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol decamer, 12 kDa
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.3 nm
Dmax 6.2 nm

SASDJN9 – Saposin-A plus MNA C13 (1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol)

UniProt ID: None (60-140) Prosaposin (Saposin-A)

UniProt ID: None (None-None) 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol
Prosaposin (Saposin-A)1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol experimental SAS data
Prosaposin (Saposin-A) 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol Kratky plot
Sample: Prosaposin (Saposin-A) dimer, 19 kDa Homo sapiens protein
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol decamer, 12 kDa
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.5 nm
Dmax 6.7 nm

SASDJP9 – Saposin-A plus LMNG (2,2-didecylpropane-1,3-bis-β-D-maltopyranoside)

UniProt ID: None (60-140) Prosaposin (Saposin-A)

UniProt ID: None (None-None) 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside
Prosaposin (Saposin-A)2,2-didecylpropane-1,3-bis-β-D-maltopyranoside experimental SAS data
Prosaposin (Saposin-A) 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside Kratky plot
Sample: Prosaposin (Saposin-A) dimer, 19 kDa Homo sapiens protein
2,2-didecylpropane-1,3-bis-β-D-maltopyranoside 0, 15 kDa
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.5 nm
Dmax 7.0 nm

SASDJQ9 – Saposin-B, R232W mutant

UniProt ID: P07602 (195-272) Prosaposin (Saposin-B, R232W mutant)
Prosaposin (Saposin-B, R232W mutant) experimental SAS data
Prosaposin (Saposin-B, R232W mutant) Kratky plot
Sample: Prosaposin (Saposin-B, R232W mutant) dimer, 18 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.0 nm
Dmax 5.8 nm

SASDJR9 – Saposin-B, R232W mutant, plus MNA C12 (1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol)

UniProt ID: None (None-None) 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol

UniProt ID: P07602 (195-272) Prosaposin (Saposin-B, R232W mutant)
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitolProsaposin (Saposin-B, R232W mutant) experimental SAS data
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol Prosaposin (Saposin-B, R232W mutant) Kratky plot
Sample: 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-dodecyl-d-mannitol decamer, 12 kDa
Prosaposin (Saposin-B, R232W mutant) dimer, 18 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.3 nm
Dmax 6.9 nm

SASDJS9 – Saposin-B, R232W mutant, plus MNA C13 (1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol)

UniProt ID: None (None-None) 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol

UniProt ID: P07602 (195-272) Prosaposin (Saposin-B, R232W mutant)
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitolProsaposin (Saposin-B, R232W mutant) experimental SAS data
1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol Prosaposin (Saposin-B, R232W mutant) Kratky plot
Sample: 1,2,5,6-tetra-β-D-glucopyranoside-3,4-O-Di-tridecyl-d-mannitol decamer, 12 kDa
Prosaposin (Saposin-B, R232W mutant) dimer, 18 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.6 nm
Dmax 7.7 nm

SASDJT9 – Saposin-B, R232W mutant, plus LMNG (2,2-didecylpropane-1,3-bis-β-D-maltopyranoside)

UniProt ID: None (None-None) 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside

UniProt ID: P07602 (195-272) Prosaposin (Saposin-B, R232W mutant)
2,2-didecylpropane-1,3-bis-β-D-maltopyranosideProsaposin (Saposin-B, R232W mutant) experimental SAS data
2,2-didecylpropane-1,3-bis-β-D-maltopyranoside Prosaposin (Saposin-B, R232W mutant) Kratky plot
Sample: 2,2-didecylpropane-1,3-bis-β-D-maltopyranoside 0, 15 kDa
Prosaposin (Saposin-B, R232W mutant) dimer, 18 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 2.8 nm
Dmax 8.9 nm

SASDK52 – TAF1 tandem bromodomain

UniProt ID: P21675 (1373-1635) Transcription initiation factor II D
Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 26
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
Karim RM, Yang L, Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 2.6 nm
Dmax 7.3 nm
VolumePorod 50 nm3

SASDK62 – TAF1 tandem bromodomain with inhibitor AZD6738

UniProt ID: P21675 (1373-1635) Transcription initiation factor II D
Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
Karim RM, Yang L, Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 2.3 nm
Dmax 6.6 nm
VolumePorod 44 nm3

SASDK72 – TAF1 tandem bromodomain with inhibitor BAY299

UniProt ID: P21675 (1373-1635) Transcription initiation factor II D
Transcription initiation factor II D experimental SAS data
Transcription initiation factor II D Kratky plot
Sample: Transcription initiation factor II D monomer, 31 kDa Escherichia coli protein
Buffer: 50 mM HEPES, 5% v/v ethylene glycol, 2.5% v/v DMSO and 2 mM DTT, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2019 Oct 19
Discovery of Dual TAF1-ATR Inhibitors and Ligand-Induced Structural Changes of the TAF1 Tandem Bromodomain. J Med Chem 65(5):4182-4200 (2022)
Karim RM, Yang L, Chen L, Bikowitz MJ, Lu J, Grassie D, Shultz ZP, Lopchuk JM, Chen J, Schönbrunn E
RgGuinier 3.6 nm
Dmax 12.5 nm
VolumePorod 106 nm3