SASBDB entries for UniProt ID:

SASDJA9 – Sensory rhodopsin II - transducer complex (NpSRII/NpHtrII) in detergent at 4000 mM NaCl studied with SANS

UniProt ID: P42196 (1-239) Sensory rhodopsin II from Natronbacterium pharaonis

UniProt ID: P42259 (3-534) Sensory rhodopsin II transducer from Natronomonas pharaonis
Sensory rhodopsin II from Natronbacterium pharaonisSensory rhodopsin II transducer from Natronomonas pharaonis experimental SAS data
GASBOR model
Sample: Sensory rhodopsin II from Natronbacterium pharaonis dimer, 53 kDa Natronomonas pharaonis protein
Sensory rhodopsin II transducer from Natronomonas pharaonis dimer, 116 kDa Natronomonas pharaonis protein
Buffer: 4000 mM NaCl, 100 mM Na/Na-Pi, 1.0 mM EDTA, 0.05% DDM (D2O buffer), pH: 8
Experiment: SANS data collected at YuMO SANS TOF spectrometer, IBR-2, Frank Laboratory of Neutron Physics, Joint Institute for Nuclear Research on 2019 Jan 25
Ambiguities in and completeness of SAS data analysis of membrane proteins: the case of the sensory rhodopsin II–transducer complex Acta Crystallographica Section D Structural Biology 77(11) (2021)
Ryzhykau Y, Vlasov A, Orekhov P, Rulev M, Rogachev A, Vlasova A, Kazantsev A, Verteletskiy D, Skoi V, Brennich M, Pernot P, Murugova T, Gordeliy V, Kuklin A
RgGuinier 10.4 nm
Dmax 39.0 nm

SASDJB9 – LIM/homeobox protein Lhx3 homeodomain (Lhx3-HD): HD3

UniProt ID: P50481 (148-227) LIM/homeobox protein Lhx3
LIM/homeobox protein Lhx3 experimental SAS data
MONSA model
Sample: LIM/homeobox protein Lhx3 monomer, 10 kDa Mus musculus protein
Buffer: 20 mM sodium phosphate monobasic/dibasic, 100 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Nov 2
Contrasting DNA-binding behaviour by ISL1 and LHX3 underpins differential gene targeting in neuronal cell specification Journal of Structural Biology: X :100043 (2020)
Smith N, Wilkinson-White L, Kwan A, Trewhella J, Matthews J
RgGuinier 2.1 nm
Dmax 7.0 nm
VolumePorod 18 nm3

SASDJC9 – Insulin gene enhancer protein Isl-1 LIM-interaction domain (Isl1-LID) fused to the LIM/homeobox protein Lhx3 LIM-homeodomain (Lhx3-LIMHD): LLHD3

UniProt ID: P50481 (28-227) LIM/homeobox protein Lhx3

UniProt ID: P61372 (262-291) Insulin gene enhancer protein ISL-1
LIM/homeobox protein Lhx3Insulin gene enhancer protein ISL-1 experimental SAS data
MONSA model
Sample: LIM/homeobox protein Lhx3 monomer, 23 kDa Mus musculus protein
Insulin gene enhancer protein ISL-1 monomer, 4 kDa Mus musculus protein
Buffer: 20 mM sodium phosphate monobasic/dibasic, 100 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Nov 2
Contrasting DNA-binding behaviour by ISL1 and LHX3 underpins differential gene targeting in neuronal cell specification Journal of Structural Biology: X :100043 (2020)
Smith N, Wilkinson-White L, Kwan A, Trewhella J, Matthews J
RgGuinier 3.3 nm
Dmax 12.0 nm
VolumePorod 39 nm3

SASDJD9 – Insulin gene enhancer protein Isl-1 homeodomain (Isl1-HD) fused to the LIM/homeobox protein Lhx3 homeodomain (Lhx3-HD): 2HD

UniProt ID: P61372 (164-261) Insulin gene enhancer protein ISL-1

UniProt ID: P50481 (148-227) LIM/homeobox protein Lhx3
Insulin gene enhancer protein ISL-1LIM/homeobox protein Lhx3 experimental SAS data
MONSA model
Sample: Insulin gene enhancer protein ISL-1 monomer, 12 kDa Mus musculus protein
LIM/homeobox protein Lhx3 monomer, 9 kDa Mus musculus protein
Buffer: 20 mM sodium phosphate monobasic/dibasic, 100 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Nov 2
Contrasting DNA-binding behaviour by ISL1 and LHX3 underpins differential gene targeting in neuronal cell specification Journal of Structural Biology: X :100043 (2020)
Smith N, Wilkinson-White L, Kwan A, Trewhella J, Matthews J
RgGuinier 3.4 nm
Dmax 12.7 nm
VolumePorod 40 nm3

SASDJE9 – Insulin gene enhancer protein Isl-1 homeodomain/LIM-interaction domain (Isl1-HDLID) fused to the LIM/homeobox protein Lhx3 LIM-homeodomain (Lhx3-LIMHD): 2HDLL

UniProt ID: P61372 (179-291) Insulin gene enhancer protein ISL-1

UniProt ID: P50481 (28-227) LIM/homeobox protein Lhx3
Insulin gene enhancer protein ISL-1LIM/homeobox protein Lhx3 experimental SAS data
MONSA model
Sample: Insulin gene enhancer protein ISL-1 monomer, 14 kDa Mus musculus protein
LIM/homeobox protein Lhx3 monomer, 23 kDa Mus musculus protein
Buffer: 20 mM sodium phosphate monobasic/dibasic, 100 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Nov 2
Contrasting DNA-binding behaviour by ISL1 and LHX3 underpins differential gene targeting in neuronal cell specification Journal of Structural Biology: X :100043 (2020)
Smith N, Wilkinson-White L, Kwan A, Trewhella J, Matthews J
RgGuinier 3.4 nm
Dmax 13.0 nm
VolumePorod 76 nm3

SASDJF9 – M100 DNA oligonucleotide bound to the LIM/homeobox protein Lhx3 homeodomain (Lhx3-HD): M100 + HD3

UniProt ID: None (None-None) M100 oligonucleotide

UniProt ID: P50481 (148-227) LIM/homeobox protein Lhx3
M100 oligonucleotideLIM/homeobox protein Lhx3 experimental SAS data
MONSA model
Sample: M100 oligonucleotide monomer, 12 kDa DNA
LIM/homeobox protein Lhx3 monomer, 10 kDa Mus musculus protein
Buffer: 20 mM sodium phosphate monobasic/dibasic, 100 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Nov 2
Contrasting DNA-binding behaviour by ISL1 and LHX3 underpins differential gene targeting in neuronal cell specification Journal of Structural Biology: X :100043 (2020)
Smith N, Wilkinson-White L, Kwan A, Trewhella J, Matthews J
RgGuinier 2.1 nm
Dmax 7.0 nm
VolumePorod 27 nm3

SASDJG9 – M100 DNA oligonucleotide bound to the protein fusion of Insulin gene enhancer protein Isl-1 LIM-interaction domain (Isl1-LID) and LIM/homeobox protein Lhx3 LIM-homeodomain (Lhx3-LIMHD): M100 + LLHD3

UniProt ID: None (None-None) M100 oligonucleotide

UniProt ID: P50481 (28-227) LIM/homeobox protein Lhx3

UniProt ID: P61372 (262-291) Insulin gene enhancer protein ISL-1
M100 oligonucleotideLIM/homeobox protein Lhx3Insulin gene enhancer protein ISL-1 experimental SAS data
MONSA model
Sample: M100 oligonucleotide monomer, 12 kDa DNA
LIM/homeobox protein Lhx3 monomer, 23 kDa Mus musculus protein
Insulin gene enhancer protein ISL-1 monomer, 4 kDa Mus musculus protein
Buffer: 20 mM sodium phosphate monobasic/dibasic, 100 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Nov 2
Contrasting DNA-binding behaviour by ISL1 and LHX3 underpins differential gene targeting in neuronal cell specification Journal of Structural Biology: X :100043 (2020)
Smith N, Wilkinson-White L, Kwan A, Trewhella J, Matthews J
RgGuinier 3.6 nm
Dmax 13.0 nm
VolumePorod 49 nm3

SASDJH9 – M100 DNA oligonucleotide bound to the protein fusion of Insulin gene enhancer protein Isl-1 homeodomain (Isl1-HD) and LIM/homeobox protein Lhx3 homeodomain (Lhx3-HD): M100 + 2HD

UniProt ID: None (None-None) M100 oligonucleotide

UniProt ID: P61372 (164-261) Insulin gene enhancer protein ISL-1

UniProt ID: P50481 (148-227) LIM/homeobox protein Lhx3
M100 oligonucleotideInsulin gene enhancer protein ISL-1LIM/homeobox protein Lhx3 experimental SAS data
MONSA model
Sample: M100 oligonucleotide monomer, 12 kDa DNA
Insulin gene enhancer protein ISL-1 monomer, 12 kDa Mus musculus protein
LIM/homeobox protein Lhx3 monomer, 9 kDa Mus musculus protein
Buffer: 20 mM sodium phosphate monobasic/dibasic, 100 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Nov 2
Contrasting DNA-binding behaviour by ISL1 and LHX3 underpins differential gene targeting in neuronal cell specification Journal of Structural Biology: X :100043 (2020)
Smith N, Wilkinson-White L, Kwan A, Trewhella J, Matthews J
RgGuinier 2.4 nm
Dmax 7.5 nm
VolumePorod 41 nm3

SASDJJ9 – M100 DNA oligonucleotide bound to the protein fusion of Insulin gene enhancer protein Isl-1 homeodomain/LIM-interaction domain (Isl1-HDLID) and LIM/homeobox protein Lhx3 LIM-homeodomain (Lhx3-LIMHD): M100 + 2HDLL

UniProt ID: None (None-None) M100 oligonucleotide

UniProt ID: P61372 (179-291) Insulin gene enhancer protein ISL-1

UniProt ID: P50481 (28-227) LIM/homeobox protein Lhx3
M100 oligonucleotideInsulin gene enhancer protein ISL-1LIM/homeobox protein Lhx3 experimental SAS data
MONSA model
Sample: M100 oligonucleotide monomer, 12 kDa DNA
Insulin gene enhancer protein ISL-1 monomer, 14 kDa Mus musculus protein
LIM/homeobox protein Lhx3 monomer, 23 kDa Mus musculus protein
Buffer: 20 mM sodium phosphate monobasic/dibasic, 100 mM NaCl, 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Nov 2
Contrasting DNA-binding behaviour by ISL1 and LHX3 underpins differential gene targeting in neuronal cell specification Journal of Structural Biology: X :100043 (2020)
Smith N, Wilkinson-White L, Kwan A, Trewhella J, Matthews J
RgGuinier 3.6 nm
Dmax 14.0 nm
VolumePorod 70 nm3

SASDJL9 – Saposin-A monomer

UniProt ID: P07602 (60-140) Prosaposin (Saposin A)
Prosaposin (Saposin A) experimental SAS data
Prosaposin (Saposin A) Kratky plot
Sample: Prosaposin (Saposin A) monomer, 10 kDa Homo sapiens protein
Buffer: 25 mM Tris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at 12-ID-B SAXS/WAXS, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Feb 18
Stable Picodisc Assemblies from Saposin Proteins and Branched Detergents. Biochemistry 60(14):1108-1119 (2021)
Kurgan KW, Chen B, Brown KA, Falco Cobra P, Ye X, Ge Y, Gellman SH
RgGuinier 1.5 nm
Dmax 5.0 nm
VolumePorod 23 nm3