SASBDB entries for UniProt ID:

SASDK82 – Full-length nonstructural protein 2 (nsP2) of Chikungunya virus (CHIKV)

UniProt ID: F2YI08-1 (None-None) NsP2 protein
NsP2 protein experimental SAS data
CORAL model
Sample: NsP2 protein monomer, 90 kDa Chikungunya virus protein
Buffer: 20 mM Hepes pH 7.4, 150 mM NaCl, 1 mM DTT, 5% glycerol, pH: 7.4
Experiment: SAXS data collected at 23A, Taiwan Photon Source, NSRRC on 2019 Aug 10
Inter-domain Flexibility of Chikungunya Virus nsP2 Helicase-Protease Differentially Influences Viral RNA Replication and Infectivity. J Virol (2020)
Law YS, Wang S, Tan YB, Shih O, Utt A, Goh WY, Lian BJ, Chen MW, Jeng US, Merits A, Luo D
RgGuinier 3.9 nm
Dmax 12.0 nm
VolumePorod 134 nm3

SASDK92 – Interferon-activable protein 204 from Mus musculus (Mouse) amino-acids 215-619

UniProt ID: P0DOV2 (215-619) Interferon-activable protein 204
Interferon-activable protein 204 experimental SAS data
CHIMERA model
Sample: Interferon-activable protein 204 monomer, 47 kDa Mus musculus protein
Buffer: 20 mM HEPES, 100 mM KCl, pH: 7.4
Experiment: SAXS data collected at X9A, National Synchrotron Light Source (NSLS) on 2013 Mar 14
Structural mechanism of DNA recognition by the p204 HIN domain. Nucleic Acids Res (2021)
Fan X, Jiang J, Zhao D, Chen F, Ma H, Smith P, Unterholzner L, Xiao TS, Jin T
RgGuinier 3.1 nm
Dmax 9.5 nm
VolumePorod 28 nm3

SASDKA2 – Two titin I27 domains linked with RS linker

UniProt ID: Q8WZ42 (None-None) Two titin I27 domains linked with RS linker
Two titin I27 domains linked with RS linker experimental SAS data
Two titin I27 domains linked with RS linker Rg histogram
Sample: Two titin I27 domains linked with RS linker monomer, 23 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 3.0 nm

SASDKB2 – Two titin I27 domains linked with RS linker

UniProt ID: Q8WZ42 (None-None) Two titin I27 domains linked with RS linker
Two titin I27 domains linked with RS linker experimental SAS data
Sample: Two titin I27 domains linked with RS linker monomer, 23 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 3.0 nm

SASDKC2 – Two titin I27 domains linked with GGG linker

UniProt ID: Q8WZ42 (None-None) Two titin I27 domains linked with GGG linker
Two titin I27 domains linked with GGG linker experimental SAS data
Two titin I27 domains linked with GGG linker Rg histogram
Sample: Two titin I27 domains linked with GGG linker monomer, 23 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 2.9 nm

SASDKD2 – Two titin I27 domains linked with VKA linker

UniProt ID: Q8WZ42 (None-None) Two titin I27 domains linked with VKA linker
Two titin I27 domains linked with VKA linker experimental SAS data
Two titin I27 domains linked with VKA linker Rg histogram
Sample: Two titin I27 domains linked with VKA linker monomer, 24 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 3.1 nm

SASDKE2 – Two titin I27 domains linked with AAE linker

UniProt ID: Q8WZ42 (None-None) Two titin I27 domains linked with AAE linker
Two titin I27 domains linked with AAE linker experimental SAS data
Two titin I27 domains linked with AAE linker Rg histogram
Sample: Two titin I27 domains linked with AAE linker monomer, 24 kDa protein
Buffer: 50 mM potassium phosphate, 300 mM KCl, DTT 2mM, pH: 7.6
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Dec 4
Interdomain linkers tailor the stability of immunoglobulin repeats in polyproteins. Biochem Biophys Res Commun 550:43-48 (2021)
Joshi T, Garg S, Estaña A, Cortés J, Bernadó P, Das S, Kammath AR, Sagar A, Rakshit S
RgGuinier 3.1 nm

SASDKH2 – E2F1/DP1 in complex with a 15-mer DNA oligo

UniProt ID: Q01094 (121-301) Transcription factor E2F1

UniProt ID: None (None-None) 15-mer DNA oligo of the human E2F1 promoter

UniProt ID: Q14186 (105-353) E2F dimerization partner 1
Transcription factor E2F115-mer DNA oligo of the human E2F1 promoterE2F dimerization partner 1 experimental SAS data
CUSTOM IN-HOUSE model
Sample: Transcription factor E2F1 monomer, 20 kDa Homo sapiens protein
15-mer DNA oligo of the human E2F1 promoter monomer, 9 kDa DNA
E2F dimerization partner 1 monomer, 29 kDa Homo sapiens protein
Buffer: 50 mM Tris-Cl pH 8.0, 150 mM NaCl and 2 mM DTT, pH: 8
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Oct 19
High Conformational Flexibility of the E2F1/DP1/DNA Complex. J Mol Biol 433(18):167119 (2021)
Saad D, Paissoni C, Chaves-Sanjuan A, Nardini M, Mantovani R, Gnesutta N, Camilloni C
RgGuinier 3.7 nm
Dmax 11.8 nm
VolumePorod 99 nm3

SASDKJ2 – Testican-2 (23-379) Ca-free

UniProt ID: Q92563 (23-379) Testican-2
Testican-2 experimental SAS data
DAMMIN model
Sample: Testican-2 monomer, 40 kDa Homo sapiens protein
Buffer: 20 mM HEPES (Na), 130 mM NaCl, 5 % (v/v) glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 18
The Central Region of Testican-2 Forms a Compact Core and Promotes Cell Migration International Journal of Molecular Sciences 21(24):9413 (2020)
Krajnc A, Gaber A, Lenarčič B, Pavšič M
RgGuinier 3.4 nm
Dmax 14.0 nm
VolumePorod 82 nm3

SASDKK2 – Testican-2 (23-379) Ca-loaded

UniProt ID: Q92563 (23-379) Testican-2
Testican-2 experimental SAS data
Testican-2 (23-379) Ca-loaded Rg histogram
Sample: Testican-2 monomer, 40 kDa Homo sapiens protein
Buffer: 20 mM HEPES (Na), 130 mM NaCl, 3 mM CaCl2, 5 % (v/v) glycerol, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2016 Oct 18
The Central Region of Testican-2 Forms a Compact Core and Promotes Cell Migration International Journal of Molecular Sciences 21(24):9413 (2020)
Krajnc A, Gaber A, Lenarčič B, Pavšič M
RgGuinier 3.2 nm
Dmax 12.6 nm
VolumePorod 78 nm3