SASBDB entries for UniProt ID:

SASDLL5 – frataxin homolog, Yfh1, at 0 °C

UniProt ID: Q07540 (52-174) Frataxin homolog, mitochondrial
Frataxin homolog, mitochondrial experimental SAS data
frataxin homolog, Yfh1, at 0 °C Rg histogram
Sample: Frataxin homolog, mitochondrial monomer, 14 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM HEPES, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Nov 28
The role of hydration in protein stability: comparison of the cold and heat unfolded states of Yfh1. J Mol Biol 417(5):413-24 (2012)
Adrover M, Martorell G, Martin SR, Urosev D, Konarev PV, Svergun DI, Daura X, Temussi P, Pastore A
RgGuinier 2.2 nm

SASDLM5 – frataxin homolog, Yfh1, at 20 °C

UniProt ID: Q07540 (52-174) Frataxin homolog, mitochondrial
Frataxin homolog, mitochondrial experimental SAS data
frataxin homolog, Yfh1, at 20 °C Rg histogram
Sample: Frataxin homolog, mitochondrial monomer, 14 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM HEPES, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Nov 28
The role of hydration in protein stability: comparison of the cold and heat unfolded states of Yfh1. J Mol Biol 417(5):413-24 (2012)
Adrover M, Martorell G, Martin SR, Urosev D, Konarev PV, Svergun DI, Daura X, Temussi P, Pastore A
RgGuinier 2.1 nm

SASDLN5 – frataxin homolog, Yfh1, at 50 °C

UniProt ID: Q07540 (52-174) Frataxin homolog, mitochondrial
Frataxin homolog, mitochondrial experimental SAS data
frataxin homolog, Yfh1, at 50 °C Rg histogram
Sample: Frataxin homolog, mitochondrial monomer, 14 kDa Saccharomyces cerevisiae (strain … protein
Buffer: 20 mM HEPES, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2008 Nov 28
The role of hydration in protein stability: comparison of the cold and heat unfolded states of Yfh1. J Mol Biol 417(5):413-24 (2012)
Adrover M, Martorell G, Martin SR, Urosev D, Konarev PV, Svergun DI, Daura X, Temussi P, Pastore A
RgGuinier 2.5 nm

SASDLP5 – Complex of the josephin domain of ataxin-3 with ubiquitin

UniProt ID: None (None-None) Josephin domain of ataxin-3

UniProt ID: P0CG47 (1-76) Polyubiquitin-B
Josephin domain of ataxin-3Polyubiquitin-B experimental SAS data
DAMMIF model
Sample: Josephin domain of ataxin-3 monomer, 21 kDa Homo sapiens protein
Polyubiquitin-B monomer, 9 kDa Homo sapiens protein
Buffer: 20 mM Na-phosphate, 2 mM DTT, pH: 6.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Oct 7
Allosteric regulation of deubiquitylase activity through ubiquitination. Front Mol Biosci 2:2 (2015)
Faggiano S, Menon RP, Kelly GP, Todi SV, Scaglione KM, Konarev PV, Svergun DI, Paulson HL, Pastore A
RgGuinier 2.1 nm
Dmax 6.5 nm
VolumePorod 45 nm3

SASDLQ5 – Tricorn protease oligomers

UniProt ID: P96086 (1-1071) Tricorn protease
Tricorn protease experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Tricorn protease hexamer, 730 kDa Thermoplasma acidophilum (strain … protein
Buffer: 20mM Tris-HCl, 100 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2003 Jan 14
X-ray snapshots of peptide processing in mutants of tricorn-interacting factor F1 from Thermoplasma acidophilum. J Biol Chem 280(39):33387-96 (2005)
Goettig P, Brandstetter H, Groll M, Göhring W, Konarev PV, Svergun DI, Huber R, Kim JS
RgGuinier 5.9 nm

SASDLR5 – Multidomain Regulatory Protein Rv1364c (monomer)

UniProt ID: P9WLZ7 (1-653) Multidomain regulatory protein Rv1364c
Multidomain regulatory protein Rv1364c experimental SAS data
GASBOR model
Sample: Multidomain regulatory protein Rv1364c monomer, 70 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 25 mM Tris-HCl, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Feb 14
Structural characterization of the multidomain regulatory protein Rv1364c from Mycobacterium tuberculosis. Structure 19(1):56-69 (2011)
King-Scott J, Konarev PV, Panjikar S, Jordanova R, Svergun DI, Tucker PA
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 130 nm3

SASDLS5 – Multidomain Regulatory Protein Rv1364c (dimer)

UniProt ID: P9WLZ7 (1-653) Multidomain regulatory protein Rv1364c
Multidomain regulatory protein Rv1364c experimental SAS data
GASBOR model
Sample: Multidomain regulatory protein Rv1364c dimer, 139 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 25 mM Tris-HCl, 50 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Feb 14
Structural characterization of the multidomain regulatory protein Rv1364c from Mycobacterium tuberculosis. Structure 19(1):56-69 (2011)
King-Scott J, Konarev PV, Panjikar S, Jordanova R, Svergun DI, Tucker PA
RgGuinier 4.5 nm
Dmax 18.0 nm
VolumePorod 289 nm3

SASDLT5 – Modification methylase SsoII (M.SsoII) protein bound to 12-bp DNA

UniProt ID: P34879 (1-379) Modification methylase SsoII

UniProt ID: None (None-None) 12-bp DNA
Modification methylase SsoII12-bp DNA experimental SAS data
Modification methylase SsoII (M.SsoII) protein bound to 12-bp DNA Rg histogram
Sample: Modification methylase SsoII monomer, 43 kDa Shigella sonnei protein
12-bp DNA monomer, 8 kDa DNA
Buffer: 50 mM Na-phosphate buffer, pH: 7
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Mar 13
Flexibility of the linker between the domains of DNA methyltransferase SsoII revealed by small-angle X-ray scattering: implications for transcription regulation in SsoII restriction-modification system. PLoS One 9(4):e93453 (2014)
Konarev PV, Kachalova GS, Ryazanova AY, Kubareva EA, Karyagina AS, Bartunik HD, Svergun DI
RgGuinier 2.8 nm
Dmax 11.0 nm
VolumePorod 85 nm3

SASDLU5 – Complex formed between the replication origin-binding protein UL9 (C-terminal domains) with 15-bp DNA

UniProt ID: P10193 (487-851) Replication origin-binding protein

UniProt ID: None (None-None) 15-bp DNA
Replication origin-binding protein15-bp DNA experimental SAS data
MONSA model
Sample: Replication origin-binding protein dimer, 81 kDa Human herpesvirus 1 … protein
15-bp DNA monomer, 9 kDa DNA
Buffer: 20 mM Tris-HCl, 50 mM NaCl, 5 mM MgCl2, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2006 May 27
Structural and biophysical characterization of the proteins interacting with the herpes simplex virus 1 origin of replication. J Biol Chem 284(24):16343-16353 (2009)
Manolaridis I, Mumtsidu E, Konarev P, Makhov AM, Fullerton SW, Sinz A, Kalkhof S, McGeehan JE, Cary PD, Griffith JD, Svergun D, Kneale GG, Tucker PA
RgGuinier 3.6 nm
Dmax 15.0 nm

SASDLV5 – Myosin Filament-Linking Protein Myomesin-1 (domains My9-My13, dimer)

UniProt ID: P52179 (1152-1685) Myomesin-1
Myomesin-1 experimental SAS data
DAMMIN model
Sample: Myomesin-1 dimer, 121 kDa Homo sapiens protein
Buffer: 25 mMTris-HCl, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 May 10
Superhelical architecture of the myosin filament-linking protein myomesin with unusual elastic properties. PLoS Biol 10(2):e1001261 (2012)
Pinotsis N, Chatziefthimiou SD, Berkemeier F, Beuron F, Mavridis IM, Konarev PV, Svergun DI, Morris E, Rief M, Wilmanns M
RgGuinier 9.6 nm
Dmax 37.0 nm