SASBDB entries for UniProt ID:

SASDLW5 – Cytochrome c nitrite reductase from Thioalkalivibrio nitratireducens

UniProt ID: L0DSL2 (29-553) Cytochrome c-552
Cytochrome c-552 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cytochrome c-552 hexamer, 356 kDa Thioalkalivibrio nitratireducens (strain … protein
Buffer: Tris-borate buffer, pH: 8.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 May 8
Isolation and oligomeric composition of cytochrome c nitrite reductase from the haloalkaliphilic bacterium Thioalkalivibrio nitratireducens. Biochemistry (Mosc) 73(2):164-70 (2008)
Tikhonova TV, Slutskaya ES, Filimonenkov AA, Boyko KM, Kleimenov SY, Konarev PV, Polyakov KM, Svergun DI, Trofimov AA, Khomenkov VG, Zvyagilskaya RA, Popov VO
RgGuinier 4.8 nm

SASDLX5 – Wild-type human mitochondrial deoxyuridine 5'-triphosphate nucleotidohydrolase (dUTPase)

UniProt ID: P33316 (1-252) Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial experimental SAS data
BUNCH model
Sample: Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial trimer, 80 kDa Homo sapiens protein
Buffer: 20 mM citrate buffer, 1 mM DTT, 0.1 mM PMSF and 1 mM MgCl2, pH: 5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2005 Feb 1
Molecular shape and prominent role of β-strand swapping in organization of dUTPase oligomers FEBS Letters 583(5):865-871 (2009)
Takács E, Barabás O, Petoukhov M, Svergun D, Vértessy B
RgGuinier 2.6 nm
Dmax 7.0 nm
VolumePorod 101 nm3

SASDLY5 – Monomer-dimer equilibrium of glutamyl-tRNA synthetase

UniProt ID: P9WFV9 (1-490) Glutamate--tRNA ligase
Glutamate--tRNA ligase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Glutamate--tRNA ligase dimer, 108 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 35 mM HEPES⁄NaOH, pH: 6.5
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Feb 19
Kinetic and mechanistic characterization of Mycobacterium tuberculosis glutamyl-tRNA synthetase and determination of its oligomeric structure in solution FEBS Journal 276(5):1398-1417 (2009)
Paravisi S, Fumagalli G, Riva M, Morandi P, Morosi R, Konarev P, Petoukhov M, Bernier S, Chênevert R, Svergun D, Curti B, Vanoni M
RgGuinier 3.5 nm
Dmax 11.0 nm
VolumePorod 123 nm3

SASDLZ5 – Hepatocyte growth factor/Hepatocyte growth factor receptor (NK1-Met567) complex

UniProt ID: P14210 (38-209) Hepatocyte growth factor

UniProt ID: P08581 (25-562) Hepatocyte growth factor receptor
Hepatocyte growth factorHepatocyte growth factor receptor experimental SAS data
MONSA model
Sample: Hepatocyte growth factor dimer, 39 kDa Homo sapiens protein
Hepatocyte growth factor receptor dimer, 122 kDa Homo sapiens protein
Buffer: Phosphate-buffered saline containing EDTA, surfactant P20 and 5 M NaCl, pH: 6
Experiment: SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Jun 26
Engineering the NK1 Fragment of Hepatocyte Growth Factor/Scatter Factor as a MET Receptor Antagonist Journal of Molecular Biology 377(3):616-622 (2008)
Youles M, Holmes O, Petoukhov M, Nessen M, Stivala S, Svergun D, Gherardi E
RgGuinier 5.3 nm
Dmax 20.0 nm
VolumePorod 290 nm3

SASDL26 – CBD12 of CALX1.1 (apo state)

UniProt ID: Q9VDG5 (442-697) AT07459p
AT07459p experimental SAS data
CBD12 of CALX1.1 (apo state) Rg histogram
Sample: AT07459p monomer, 30 kDa Drosophila melanogaster protein
Buffer: 20 mM Tris, 5 mM β-mercaptoethanol, 200 mM NaCl, 1% v/v glycerol, pH: 7.4
Experiment: SAXS data collected at Xenocs Xeuss, Institute of Physics, University of São Paulo on 2018 Mar 8
Molecular insights on CALX-CBD12 inter-domain dynamics from MD simulations, RDCs and SAXS. Biophys J (2021)
de Souza Degenhardt MF, Vitale PAM, Abiko LA, Zacharias M, Sattler M, Oliveira CLP, Salinas RK
RgGuinier 2.5 nm
Dmax 8.7 nm
VolumePorod 41 nm3

SASDL36 – CBD12 of CALX1.1 in the calcium-bound state

UniProt ID: Q9VDG5 (442-697) AT07459p
AT07459p experimental SAS data
CBD12 of CALX1.1 in the calcium-bound state Rg histogram
Sample: AT07459p monomer, 30 kDa Drosophila melanogaster protein
Buffer: 20 mM Tris, 5 mM β-mercaptoethanol, 200 mM NaCl, 1% v/v glycerol, 0.8 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at Xenocs Xeuss, Institute of Physics, University of São Paulo on 2018 Apr 13
Molecular insights on CALX-CBD12 inter-domain dynamics from MD simulations, RDCs and SAXS. Biophys J (2021)
de Souza Degenhardt MF, Vitale PAM, Abiko LA, Zacharias M, Sattler M, Oliveira CLP, Salinas RK
RgGuinier 2.7 nm
Dmax 9.3 nm
VolumePorod 40 nm3

SASDL66 – High mannose neurofascin 155 immunoglobulin domains 1-6, 37.2 μM

UniProt ID: E9PW06 (25-621) Neurofascin
Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.7 nm
Dmax 18.0 nm
VolumePorod 355 nm3

SASDL76 – High mannose neurofascin 155 immunoglobulin domains 1-6, 73.5 μM

UniProt ID: E9PW06 (25-621) Neurofascin
Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 5.0 nm
Dmax 21.0 nm
VolumePorod 450 nm3

SASDL86 – High mannose neurofascin 155 immunoglobulin domains 1-6, 19.7 μM

UniProt ID: E9PW06 (25-621) Neurofascin
Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.5 nm
Dmax 17.0 nm
VolumePorod 290 nm3

SASDL96 – High mannose neurofascin 155 immunoglobulin domains 1-6, 9.5 μM

UniProt ID: E9PW06 (25-621) Neurofascin
Neurofascin experimental SAS data
Neurofascin Kratky plot
Sample: Neurofascin dimer, 139 kDa Mus musculus protein
Buffer: 25 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2019 Dec 16
Structural insights into the contactin 1 – neurofascin 155 adhesion complex Nature Communications 13(1) (2022)
Chataigner L, Gogou C, den Boer M, Frias C, Thies-Weesie D, Granneman J, Heck A, Meijer D, Janssen B
RgGuinier 4.3 nm
Dmax 17.0 nm
VolumePorod 240 nm3