SASBDB entries for UniProt ID:

SASDRU4 – Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) trimer formed in the presence of L-serine

UniProt ID: O53289 (1-409) Phosphoserine phosphatase SerB2
Phosphoserine phosphatase SerB2 experimental SAS data
Phosphoserine phosphatase SerB2 Kratky plot
Sample: Phosphoserine phosphatase SerB2 trimer, 130 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, 10 mM L-serine, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 4.0 nm
Dmax 14.3 nm
VolumePorod 200 nm3

SASDRV4 – Mycobacterium marinum phosphoserine phosphatase (MmSerB2) dimer

UniProt ID: B2HHH0 (2-411) phosphoserine phosphatase
phosphoserine phosphatase experimental SAS data
phosphoserine phosphatase Kratky plot
Sample: Phosphoserine phosphatase dimer, 88 kDa Mycobacterium marinum (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 3.2 nm
Dmax 9.9 nm
VolumePorod 138 nm3

SASDRW4 – Mycobacterium avium phosphoserine phosphatase (MaSerB) dimer

UniProt ID: A0QJI1 (2-411) Phosphoserine phosphatase (G31R, G152E)
Phosphoserine phosphatase (G31R, G152E) experimental SAS data
DADIMODO model
Sample: Phosphoserine phosphatase (G31R, G152E) dimer, 88 kDa Mycobacterium avium (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 3.3 nm
Dmax 10.1 nm
VolumePorod 138 nm3

SASDR25 – Periplasmic domain of cholera toxin transcriptional activator ToxR

UniProt ID: P15795 (201-294) Cholera toxin transcriptional activator
Cholera toxin transcriptional activator experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 16 nm3

SASDR35 – Periplasmic domain of cholera transmembrane regulatory protein ToxS

UniProt ID: P24003 (25-173) Transmembrane regulatory protein ToxS
Transmembrane regulatory protein ToxS experimental SAS data
ALPHAFOLD model
Sample: Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 2.3 nm
Dmax 7.4 nm
VolumePorod 55 nm3

SASDR45 – A complex between the periplasmic domains of cholera toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS

UniProt ID: P15795 (201-294) Cholera toxin transcriptional activator

UniProt ID: P24003 (25-173) Transmembrane regulatory protein ToxS
Cholera toxin transcriptional activatorTransmembrane regulatory protein ToxS experimental SAS data
CUSTOM IN-HOUSE model
Sample: Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein
Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 2.1 nm
Dmax 6.6 nm
VolumePorod 51 nm3

SASDR55 – A complex between the periplasmic domains of cholera toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS bound to bile salt

UniProt ID: P15795 (201-294) Cholera toxin transcriptional activator

UniProt ID: P24003 (25-173) Transmembrane regulatory protein ToxS

UniProt ID: None (None-None) bile acid: sodium cholate hydrate
Cholera toxin transcriptional activatorTransmembrane regulatory protein ToxSbile acid: sodium cholate hydrate experimental SAS data
CUSTOM IN-HOUSE model
Sample: Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein
Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein
Bile acid: sodium cholate hydrate monomer, 0 kDa
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Jul 7
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 2.4 nm
Dmax 6.6 nm
VolumePorod 60 nm3

SASDRN5 – Nucleolar RNA Chaperone-Like Protein 1 (NURC1)

UniProt ID: Q9LZ65 (1-222) AT5g04600/T32M21_200
AT5g04600/T32M21_200 experimental SAS data
GASBOR model
Sample: AT5g04600/T32M21_200 monomer, 25 kDa Arabidopsis thaliana protein
Buffer: 50 mM HNa2PO4, 300 mM NaCl, 5% glycerol (v/v), 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2020 Jun 22
Structural and functional analysis of a plant nucleolar RNA chaperone-like protein. Sci Rep 13(1):9656 (2023)
Fernandes R, Ostendorp A, Ostendorp S, Mehrmann J, Falke S, Graewert MA, Weingartner M, Kehr J, Hoth S
RgGuinier 3.5 nm
Dmax 12.4 nm
VolumePorod 66 nm3

SASDRP5 – SEC-SAXS analysis of recombinant survival motor neuron protein (flSMN) conjugated to the protein transduction domain of HIV-1 Tat protein (Tat-flSMN)

UniProt ID: Q16637 (1-294) Survival motor neuron protein (fusion construct with the protein transduction domain of the human immunodeficiency virus type 1 Tat protein)
Survival motor neuron protein (fusion construct with the protein transduction domain of the human immunodeficiency virus type 1 Tat protein) experimental SAS data
Survival motor neuron protein (fusion construct with the protein transduction domain of the human immunodeficiency virus type 1 Tat protein) Kratky plot
Sample: Survival motor neuron protein (fusion construct with the protein transduction domain of the human immunodeficiency virus type 1 Tat protein), 533 kDa synthetic construct protein
Buffer: 50 mM Tris-HCl, 500 mM NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Jun 13
Recombinant flSMN
Federico Forneris
RgGuinier 8.0 nm
Dmax 29.7 nm
VolumePorod 897 nm3

SASDRR5 – Like Early Starvation 1

UniProt ID: Q5EAH9 (57-578) At3g55760
At3g55760 experimental SAS data
GASBOR model
Sample: At3g55760 monomer, 60 kDa Arabidopsis thaliana protein
Buffer: 50 mM Tris, 150 mM NaCl, 10% glycerol, 2 mM DTT, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Dec 8
LIKE EARLY STARVATION 1 and EARLY STARVATION 1 promote and stabilize amylopectin phase transition in starch biosynthesis. Sci Adv 9(21):eadg7448 (2023)
Liu C, Pfister B, Osman R, Ritter M, Heutinck A, Sharma M, Eicke S, Fischer-Stettler M, Seung D, Bompard C, Abt MR, Zeeman SC
RgGuinier 3.7 nm
Dmax 16.0 nm
VolumePorod 121 nm3