SASBDB entries for UniProt ID:

SASDRJ4 – GAC rRNA + L11 Protein: SEC-SAXS final

UniProt ID: None (None-None) 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA

UniProt ID: P36238 (1-147) 50S ribosomal protein L11
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.6 nm
Dmax 13.0 nm
VolumePorod 48 nm3

SASDRR4 – Diffusive GAC rRNA + Mg: Equilibrium final

UniProt ID: P36238 (1-147) 50S ribosomal protein L11
50S ribosomal protein L11 experimental SAS data
50S ribosomal protein L11 Kratky plot
Sample: 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 11.0 nm
VolumePorod 27 nm3

SASDRS4 – Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) dimer

UniProt ID: O53289 (1-409) Phosphoserine phosphatase SerB2
Phosphoserine phosphatase SerB2 experimental SAS data
Phosphoserine phosphatase SerB2 Kratky plot
Sample: Phosphoserine phosphatase SerB2 dimer, 87 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 3.2 nm
Dmax 10.7 nm
VolumePorod 141 nm3

SASDRT4 – Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) tetramer

UniProt ID: O53289 (1-409) Mycobacterium tuberculosis phosphoserine phosphatase SerB2
Mycobacterium tuberculosis phosphoserine phosphatase SerB2 experimental SAS data
DADIMODO model
Sample: Mycobacterium tuberculosis phosphoserine phosphatase SerB2 tetramer, 173 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 4.0 nm
Dmax 12.5 nm
VolumePorod 307 nm3

SASDRU4 – Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) trimer formed in the presence of L-serine

UniProt ID: O53289 (1-409) Phosphoserine phosphatase SerB2
Phosphoserine phosphatase SerB2 experimental SAS data
Phosphoserine phosphatase SerB2 Kratky plot
Sample: Phosphoserine phosphatase SerB2 trimer, 130 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, 10 mM L-serine, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 4.0 nm
Dmax 14.3 nm
VolumePorod 200 nm3

SASDRV4 – Mycobacterium marinum phosphoserine phosphatase (MmSerB2) dimer

UniProt ID: B2HHH0 (2-411) phosphoserine phosphatase
phosphoserine phosphatase experimental SAS data
phosphoserine phosphatase Kratky plot
Sample: Phosphoserine phosphatase dimer, 88 kDa Mycobacterium marinum (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 3.2 nm
Dmax 9.9 nm
VolumePorod 138 nm3

SASDRW4 – Mycobacterium avium phosphoserine phosphatase (MaSerB) dimer

UniProt ID: A0QJI1 (2-411) Phosphoserine phosphatase (G31R, G152E)
Phosphoserine phosphatase (G31R, G152E) experimental SAS data
DADIMODO model
Sample: Phosphoserine phosphatase (G31R, G152E) dimer, 88 kDa Mycobacterium avium (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 3.3 nm
Dmax 10.1 nm
VolumePorod 138 nm3

SASDR25 – Periplasmic domain of cholera toxin transcriptional activator ToxR

UniProt ID: P15795 (201-294) Cholera toxin transcriptional activator
Cholera toxin transcriptional activator experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 1.6 nm
Dmax 6.0 nm
VolumePorod 16 nm3

SASDR35 – Periplasmic domain of cholera transmembrane regulatory protein ToxS

UniProt ID: P24003 (25-173) Transmembrane regulatory protein ToxS
Transmembrane regulatory protein ToxS experimental SAS data
ALPHAFOLD model
Sample: Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 2.3 nm
Dmax 7.4 nm
VolumePorod 55 nm3

SASDR45 – A complex between the periplasmic domains of cholera toxin transcriptional activator ToxR and transmembrane regulatory protein ToxS

UniProt ID: P15795 (201-294) Cholera toxin transcriptional activator

UniProt ID: P24003 (25-173) Transmembrane regulatory protein ToxS
Cholera toxin transcriptional activatorTransmembrane regulatory protein ToxS experimental SAS data
CUSTOM IN-HOUSE model
Sample: Cholera toxin transcriptional activator monomer, 12 kDa Vibrio cholerae serotype … protein
Transmembrane regulatory protein ToxS dimer, 37 kDa Vibrio cholerae serotype … protein
Buffer: 50 mM Na2HPO4, 300 mM NaCl, 3% glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Mar 6
Vibrio cholerae's ToxRS bile sensing system. Elife 12 (2023)
Gubensäk N, Sagmeister T, Buhlheller C, Geronimo BD, Wagner GE, Petrowitsch L, Gräwert MA, Rotzinger M, Berger TMI, Schäfer J, Usón I, Reidl J, Sánchez-Murcia PA, Zangger K, Pavkov-Keller T
RgGuinier 2.1 nm
Dmax 6.6 nm
VolumePorod 51 nm3