SASBDB entries for UniProt ID:

SASDRC4 – GAC rRNA + L11 Protein: Time-Resolved 100 ms

UniProt ID: None (None-None) 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA

UniProt ID: P36238 (1-147) 50S ribosomal protein L11
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.4 nm
Dmax 11.5 nm
VolumePorod 35 nm3

SASDRD4 – GAC rRNA + L11 Protein: Time-Resolved 200 ms

UniProt ID: None (None-None) 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA

UniProt ID: P36238 (1-147) 50S ribosomal protein L11
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.5 nm
Dmax 12.5 nm
VolumePorod 37 nm3

SASDRE4 – GAC rRNA + L11 Protein: Time-Resolved 316 ms

UniProt ID: None (None-None) 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA

UniProt ID: P36238 (1-147) 50S ribosomal protein L11
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.5 nm
Dmax 10.0 nm
VolumePorod 38 nm3

SASDRF4 – GAC rRNA + L11 Protein: Time-Resolved 631 ms

UniProt ID: None (None-None) 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA

UniProt ID: P36238 (1-147) 50S ribosomal protein L11
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.5 nm
Dmax 13.0 nm
VolumePorod 38 nm3

SASDRG4 – GAC rRNA + L11 Protein: Time-Resolved 1000 ms

UniProt ID: None (None-None) 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA

UniProt ID: P36238 (1-147) 50S ribosomal protein L11
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.5 nm
Dmax 12.5 nm
VolumePorod 40 nm3

SASDRH4 – GAC rRNA + L11 Protein: Time-Resolved 2000 ms

UniProt ID: None (None-None) 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA

UniProt ID: P36238 (1-147) 50S ribosomal protein L11
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2018 May 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.6 nm
Dmax 12.8 nm
VolumePorod 39 nm3

SASDRJ4 – GAC rRNA + L11 Protein: SEC-SAXS final

UniProt ID: None (None-None) 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA

UniProt ID: P36238 (1-147) 50S ribosomal protein L11
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA50S ribosomal protein L11 experimental SAS data
58 nucleotide RNA L11-binding domain from E. coli 23S rRNA 50S ribosomal protein L11 Kratky plot
Sample: 58 nucleotide RNA L11-binding domain from E. coli 23S rRNA monomer, 19 kDa Escherichia coli RNA
50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM Na-MOPSO, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2017 Nov 19
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.6 nm
Dmax 13.0 nm
VolumePorod 48 nm3

SASDRR4 – Diffusive GAC rRNA + Mg: Equilibrium final

UniProt ID: P36238 (1-147) 50S ribosomal protein L11
50S ribosomal protein L11 experimental SAS data
50S ribosomal protein L11 Kratky plot
Sample: 50S ribosomal protein L11 monomer, 16 kDa Thermus thermophilus protein
Buffer: 10 mM sodium cacodylate, 100 mM KCl, pH: 6.5
Experiment: SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2016 Dec 9
Chaotic advection mixer for capturing transient states of diverse biological macromolecular systems with time-resolved small-angle X-ray scattering IUCrJ 10(3):363-375 (2023)
Zielinski K, Katz A, Calvey G, Pabit S, Milano S, Aplin C, San Emeterio J, Cerione R, Pollack L
RgGuinier 2.2 nm
Dmax 11.0 nm
VolumePorod 27 nm3

SASDRS4 – Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) dimer

UniProt ID: O53289 (1-409) Phosphoserine phosphatase SerB2
Phosphoserine phosphatase SerB2 experimental SAS data
Phosphoserine phosphatase SerB2 Kratky plot
Sample: Phosphoserine phosphatase SerB2 dimer, 87 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 3.2 nm
Dmax 10.7 nm
VolumePorod 141 nm3

SASDRT4 – Mycobacterium tuberculosis phosphoserine phosphatase (MtSerB2) tetramer

UniProt ID: O53289 (1-409) Mycobacterium tuberculosis phosphoserine phosphatase SerB2
Mycobacterium tuberculosis phosphoserine phosphatase SerB2 experimental SAS data
DADIMODO model
Sample: Mycobacterium tuberculosis phosphoserine phosphatase SerB2 tetramer, 173 kDa Mycobacterium tuberculosis (strain … protein
Buffer: 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2021 Oct 6
A morpheein equilibrium regulates catalysis in phosphoserine phosphatase SerB2 from Mycobacterium tuberculosis. Commun Biol 6(1):1024 (2023)
Pierson E, De Pol F, Fillet M, Wouters J
RgGuinier 4.0 nm
Dmax 12.5 nm
VolumePorod 307 nm3