SASBDB entries for UniProt ID:

SASDRS5 – Early Starvation 1 from Arabidopsis thaliana

UniProt ID: F4I9G2 (96-426) Inactive purple acid phosphatase-like protein
Inactive purple acid phosphatase-like protein experimental SAS data
Inactive purple acid phosphatase-like protein Kratky plot
Sample: Inactive purple acid phosphatase-like protein monomer, 40 kDa Arabidopsis thaliana protein
Buffer: 50 mM Tris, 150 mM NaCl, 10% glycerol, 2 mM DTT, pH: 8
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Dec 8
LIKE EARLY STARVATION 1 and EARLY STARVATION 1 promote and stabilize amylopectin phase transition in starch biosynthesis. Sci Adv 9(21):eadg7448 (2023)
Liu C, Pfister B, Osman R, Ritter M, Heutinck A, Sharma M, Eicke S, Fischer-Stettler M, Seung D, Bompard C, Abt MR, Zeeman SC
RgGuinier 3.2 nm
Dmax 11.5 nm
VolumePorod 83 nm3

SASDRT5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under oxidizing conditions in the presence of ADP

UniProt ID: P30663 (2-519) Nitrogen fixation regulatory protein (Q409L)
Nitrogen fixation regulatory protein (Q409L) experimental SAS data
Sample: Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein
Buffer: 50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5
Structural insights into redox signal transduction mechanisms in the control of nitrogen fixation by the NifLA system Proceedings of the National Academy of Sciences 120(30) (2023)
Boyer N, Tokmina-Lukaszewska M, Bueno Batista M, Mus F, Dixon R, Bothner B, Peters J
RgGuinier 4.9 nm
Dmax 17.9 nm
VolumePorod 233 nm3

SASDRU5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under oxidizing conditions in the presence of ATP

UniProt ID: P30663 (2-519) Nitrogen fixation regulatory protein (Q409L)
Nitrogen fixation regulatory protein (Q409L) experimental SAS data
Sample: Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein
Buffer: 50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5
Structural insights into redox signal transduction mechanisms in the control of nitrogen fixation by the NifLA system Proceedings of the National Academy of Sciences 120(30) (2023)
Boyer N, Tokmina-Lukaszewska M, Bueno Batista M, Mus F, Dixon R, Bothner B, Peters J
RgGuinier 4.9 nm
Dmax 18.0 nm
VolumePorod 225 nm3

SASDRV5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under reducing conditions in the presence of ADP

UniProt ID: P30663 (2-519) Nitrogen fixation regulatory protein (Q409L)
Nitrogen fixation regulatory protein (Q409L) experimental SAS data
Sample: Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein
Buffer: 50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5
Structural insights into redox signal transduction mechanisms in the control of nitrogen fixation by the NifLA system Proceedings of the National Academy of Sciences 120(30) (2023)
Boyer N, Tokmina-Lukaszewska M, Bueno Batista M, Mus F, Dixon R, Bothner B, Peters J
RgGuinier 4.9 nm
Dmax 18.0 nm
VolumePorod 243 nm3

SASDRW5 – Azotobacter vinelandii nitrogen fixation regulatory protein (NifL) under reducing conditions in the presence of ATP

UniProt ID: P30663 (2-519) Nitrogen fixation regulatory protein (Q409L)
Nitrogen fixation regulatory protein (Q409L) experimental SAS data
Sample: Nitrogen fixation regulatory protein (Q409L) dimer, 115 kDa Azotobacter vinelandii protein
Buffer: 50 mM Bis-Tris, 100 mM (NH4)2SO4, 10% glycerol, 5 mM DTT, pH: 7
Experiment: SAXS data collected at BL4-2, Stanford Synchrotron Radiation Lightsource (SSRL) on 2022 Jul 5
Structural insights into redox signal transduction mechanisms in the control of nitrogen fixation by the NifLA system Proceedings of the National Academy of Sciences 120(30) (2023)
Boyer N, Tokmina-Lukaszewska M, Bueno Batista M, Mus F, Dixon R, Bothner B, Peters J
RgGuinier 5.1 nm
Dmax 17.8 nm
VolumePorod 224 nm3

SASDRX5 – Arabidopsis thaliana Toc75 Potra domains bound to synthetic antigen binding fragment sAB tc1

UniProt ID: Q9STE8 (141-449) Protein TOC75-3, chloroplastic

UniProt ID: None (None-None) synthetic antigen binding fragment tc1
Protein TOC75-3, chloroplasticsynthetic antigen binding fragment tc1 experimental SAS data
Protein TOC75-3, chloroplastic synthetic antigen binding fragment tc1 Kratky plot
Sample: Protein TOC75-3, chloroplastic monomer, 35 kDa Arabidopsis thaliana protein
Synthetic antigen binding fragment tc1 monomer, 48 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 125 nm3

SASDRY5 – Arabidopsis thaliana Toc75 Potra domains bound to synthetic antigen binding fragment sAB tc2

UniProt ID: Q9STE8 (141-449) Protein TOC75-3, chloroplastic

UniProt ID: None (None-None) synthetic antigen binding fragment tc2
Protein TOC75-3, chloroplasticsynthetic antigen binding fragment tc2 experimental SAS data
Protein TOC75-3, chloroplastic synthetic antigen binding fragment tc2 Kratky plot
Sample: Protein TOC75-3, chloroplastic monomer, 35 kDa Arabidopsis thaliana protein
Synthetic antigen binding fragment tc2 monomer, 48 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 3.8 nm
Dmax 14.0 nm
VolumePorod 125 nm3

SASDRZ5 – Arabidopsis thaliana Toc75 Potra domains bound to synthetic antigen binding fragment sAB tc3

UniProt ID: Q9STE8 (141-449) Protein TOC75-3, chloroplastic

UniProt ID: None (None-None) synthetic antigen binding fragment tc3
Protein TOC75-3, chloroplasticsynthetic antigen binding fragment tc3 experimental SAS data
Protein TOC75-3, chloroplastic synthetic antigen binding fragment tc3 Kratky plot
Sample: Protein TOC75-3, chloroplastic monomer, 35 kDa Arabidopsis thaliana protein
Synthetic antigen binding fragment tc3 monomer, 49 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 3.6 nm
Dmax 13.5 nm
VolumePorod 126 nm3

SASDR26 – Arabidopsis thaliana Toc75 Potra domains bound to synthetic antigen binding fragment sAB tc5

UniProt ID: Q9STE8 (141-449) Protein TOC75-3, chloroplastic

UniProt ID: None (None-None) synthetic antigen binding fragment tc5
Protein TOC75-3, chloroplasticsynthetic antigen binding fragment tc5 experimental SAS data
Protein TOC75-3, chloroplastic synthetic antigen binding fragment tc5 Kratky plot
Sample: Protein TOC75-3, chloroplastic monomer, 35 kDa Arabidopsis thaliana protein
Synthetic antigen binding fragment tc5 monomer, 49 kDa synthetic construct protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 3.8 nm
Dmax 13.5 nm
VolumePorod 130 nm3

SASDR36 – Pisum sativum Toc75 Potra domains

UniProt ID: Q43715 (163-440) Protein TOC75, chloroplastic
Protein TOC75, chloroplastic experimental SAS data
Protein TOC75, chloroplastic Kratky plot
Sample: Protein TOC75, chloroplastic monomer, 32 kDa Pisum sativum protein
Buffer: phosphate buffered saline, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2020 Nov 23
Characterization of synthetic antigen binding fragments targeting Toc75 for the isolation of TOC in A. thaliana and P. sativum Structure (2023)
Srinivasan K, Erramilli S, Chakravarthy S, Gonzalez A, Kossiakoff A, Noinaj N
RgGuinier 2.9 nm
Dmax 11.0 nm
VolumePorod 60 nm3