Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID:

SASDR68 – Human nicotinamide phosphoribosyltransferase Δ42-51 loop mutant (NAMPT Δ42-51) in the presence of nicotinamide

UniProt ID: P43490 (2-491) Nicotinamide phosphoribosyltransferase Δ42-51

Nicotinamide phosphoribosyltransferase Δ42-51 experimental SAS data

Nicotinamide phosphoribosyltransferase Δ42-51 Kratky plot

Sample:	Nicotinamide phosphoribosyltransferase Δ42-51 dimer, 111 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM nicotinamide, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 20

Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity. J Struct Biol :108004 (2023)
Houry D, Raasakka A, Ferrario E, Niere M, Bifulco E, Kursula P, Ziegler M

R_g^Guinier	3.2	nm
D_max	10.9	nm
Volume^Porod	151	nm³

SASDR78 – Human nicotinamide phosphoribosyltransferase Δ42-51 loop mutant (NAMPT Δ42-51) in the presence of nicotinic acid

UniProt ID: P43490 (2-491) Nicotinamide phosphoribosyltransferase Δ42-51

Sample:	Nicotinamide phosphoribosyltransferase Δ42-51 dimer, 111 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM nicotinic acid, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 20

R_g^Guinier	3.3	nm
D_max	11.2	nm
Volume^Porod	152	nm³

SASDR88 – Human nicotinamide phosphoribosyltransferase Δ42-51 loop mutant (NAMPT Δ42-51) in the presence of phosphoribosyl pyrophosphate

UniProt ID: P43490 (2-491) Nicotinamide phosphoribosyltransferase Δ42-51

Sample:	Nicotinamide phosphoribosyltransferase Δ42-51 dimer, 111 kDa Homo sapiens protein
Buffer:	20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM phosphoribosyl pyrophosphate, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 May 20

R_g^Guinier	3.2	nm
D_max	11.4	nm
Volume^Porod	154	nm³

SASDR98 – The BTB domain (wild-type) of the zinc finger and BTB domain-containing protein 8A.1-A (ZBTB8a) from Xenopus laevis

UniProt ID: Q0IH98 (1-147) Zinc finger and BTB domain-containing protein 8A.1-A

Zinc finger and BTB domain-containing protein 8A.1-A experimental SAS data

Zinc finger and BTB domain-containing protein 8A.1-A Kratky plot

Sample:	Zinc finger and BTB domain-containing protein 8A.1-A dimer, 35 kDa Xenopus laevis protein
Buffer:	20 mM Hepes, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Dec 4

Dynamic BTB-domain filaments promote clustering of ZBTB proteins. Mol Cell 84(13):2490-2510.e9 (2024)
Mance L, Bigot N, Zhamungui Sánchez E, Coste F, Martín-González N, Zentout S, Biliškov M, Pukało Z, Mishra A, Chapuis C, Arteni AA, Lateur A, Goffinont S, Gaudon V, Talhaoui I, Casuso I, Beaufour M, Garnier N, Artzner F, Cadene M, Huet S, Castaing B, Suskiewicz MJ

R_g^Guinier	22.3	nm
D_max	99.6	nm

SASDRA8 – The BTB domain (S103R mutant) of the zinc finger and BTB domain-containing protein 8A.1-A (ZBTB8a) from Xenopus laevis

UniProt ID: Q0IH98 (1-147) Zinc finger and BTB domain-containing protein 8A.1-A (S103R mutant)

Zinc finger and BTB domain-containing protein 8A.1-A (S103R mutant) experimental SAS data

Zinc finger and BTB domain-containing protein 8A.1-A (S103R mutant) Kratky plot

Sample:	Zinc finger and BTB domain-containing protein 8A.1-A (S103R mutant) dimer, 35 kDa Xenopus laevis protein
Buffer:	20 mM Hepes, 150 mM NaCl, 0.5 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Dec 4

R_g^Guinier	3.3	nm
D_max	12.8	nm
Volume^Porod	60	nm³

SASDRB8 – Human tRNA guanine transglycosylase heterodimer

UniProt ID: Q9BXR0 (1-403) Queuine tRNA-ribosyltransferase catalytic subunit 1

UniProt ID: Q9H974 (1-415) Queuine tRNA-ribosyltransferase accessory subunit 2

Queuine tRNA-ribosyltransferase catalytic subunit 1Queuine tRNA-ribosyltransferase accessory subunit 2 experimental SAS data

Human tRNA guanine transglycosylase heterodimer Rg histogram

Sample:	Queuine tRNA-ribosyltransferase catalytic subunit 1 monomer, 44 kDa Homo sapiens protein Queuine tRNA-ribosyltransferase accessory subunit 2 monomer, 47 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 100 mM NaCl, 3% (w/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Dec 1

Structural and functional investigation of tRNA guanine transglycosylase University of Göttingen Dissertation - (2023)
Katharina Sievers

R_g^Guinier	3.3	nm
D_max	11.4	nm
Volume^Porod	127	nm³

SASDRC8 – Human tRNA guanine transglycosylase bound to aspartate transfer RNA (tRNA-Asp)

UniProt ID: Q9BXR0 (1-403) Queuine tRNA-ribosyltransferase catalytic subunit 1

UniProt ID: Q9H974 (1-415) Queuine tRNA-ribosyltransferase accessory subunit 2

UniProt ID: None (None-None) transfer RNA (Aspartate)

Queuine tRNA-ribosyltransferase catalytic subunit 1Queuine tRNA-ribosyltransferase accessory subunit 2transfer RNA (Aspartate) experimental SAS data

Human tRNA guanine transglycosylase bound to aspartate transfer RNA (tRNA-Asp) Rg histogram

Sample:	Queuine tRNA-ribosyltransferase catalytic subunit 1 monomer, 44 kDa Homo sapiens protein Queuine tRNA-ribosyltransferase accessory subunit 2 monomer, 47 kDa Homo sapiens protein Transfer RNA (Aspartate) monomer, 25 kDa Homo sapiens RNA
Buffer:	20 mM HEPES, 100 mM NaCl, 3% (w/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Dec 1

Structural and functional investigation of tRNA guanine transglycosylase University of Göttingen Dissertation - (2023)
Katharina Sievers

R_g^Guinier	3.5	nm
D_max	10.9	nm
Volume^Porod	160	nm³

SASDRD8 – Drosophila melanogaster kinesin heavy chain

UniProt ID: P17210 (1-975) Kinesin heavy chain (A515T)

Kinesin heavy chain (A515T) experimental SAS data

Sample:	Kinesin heavy chain (A515T) dimer, 221 kDa Drosophila melanogaster protein
Buffer:	25 mM HEPES/KOH, 150 mM NaCl, 1 mM MgCl2, 2 mM DTT, pH: 7.3
Experiment:	SAXS data collected at BM29, ESRF on 2022 Feb 19

Tropomyosin 1-I/C coordinates kinesin-1 and dynein motors during oskar mRNA transport. Nat Struct Mol Biol (2024)
Heber S, McClintock MA, Simon B, Mehtab E, Lapouge K, Hennig J, Bullock SL, Ephrussi A

R_g^Guinier	10.0	nm
D_max	45.0	nm
Volume^Porod	920	nm³

SASDRE8 – Drosophila melanogaster kinesin heavy chain + Tropomyosin1-I/C

UniProt ID: P17210 (1-975) Kinesin heavy chain (A515T)

UniProt ID: Q95TA3 (1-441) GH09289p

Kinesin heavy chain (A515T)GH09289p experimental SAS data

Kinesin heavy chain (A515T) GH09289p Kratky plot

Sample:	Kinesin heavy chain (A515T) dimer, 221 kDa Drosophila melanogaster protein GH09289p dimer, 96 kDa Drosophila melanogaster protein
Buffer:	25 mM HEPES/KOH, 150 mM NaCl, 1 mM MgCl2, 2 mM DTT, pH: 7.3
Experiment:	SAXS data collected at BM29, ESRF on 2022 Feb 19

R_g^Guinier	8.7	nm
D_max	45.0	nm
Volume^Porod	840	nm³

SASDRF8 – Plasmodium falciparum circumsporozoite protein N-terminal domain at pH 6.6

UniProt ID: P19597 (20-101) Circumsporozoite protein

Circumsporozoite protein experimental SAS data

Sample:	Circumsporozoite protein monomer, 11 kDa Plasmodium falciparum (isolate … protein
Buffer:	50 mM MES, 500 mM NaCl, 1 mM TCEP, pH: 6.6
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 8

Biophysical characterization of the Plasmodium falciparum circumsporozoite protein's N‐terminal domain Protein Science (2023)
Geens R, Stanisich J, Beyens O, D'Hondt S, Thiberge J, Ryckebosch A, Groot A, Magez S, Vertommen D, Amino R, Winter H, Volkov A, Tompa P, Sterckx Y

R_g^Guinier	2.3	nm
D_max	9.1	nm
Volume^Porod	24	nm³

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