SASBDB entries for UniProt ID:

SASDRJ7 – HOTag6 tetramerization domain followed by a (Pro-Ala)10 linker fused to human monoubiquitin with 200 mM NaCl

UniProt ID: None (1-76) HOTag6-(PA)10-Ubiquitin
HOTag6-(PA)10-Ubiquitin experimental SAS data
HOTag6-(PA)10-Ubiquitin Kratky plot
Sample: HOTag6-(PA)10-Ubiquitin tetramer, 57 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 200 mM NaCl, 0.5 mM EDTA, 0.02% NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Feb 19
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 5.1 nm
Dmax 19.0 nm
VolumePorod 105 nm3

SASDRK7 – M1-linked tetraubiquitin, M1(1-72)-Ub4

UniProt ID: P0CG47 (1-72) Polyubiquitin-B
Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 33 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.2 nm
Dmax 12.5 nm
VolumePorod 41 nm3

SASDRL7 – M1-linked tetraubiquitin, M1(1-73)-Ub4

UniProt ID: P0CG47 (1-73) Polyubiquitin-B
Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 33 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.3 nm
Dmax 12.8 nm
VolumePorod 41 nm3

SASDRM7 – M1-linked tetraubiquitin, M1(1-74)-Ub4

UniProt ID: P0CG47 (1-74) Polyubiquitin-B
Polyubiquitin-B experimental SAS data
Polyubiquitin-B Kratky plot
Sample: Polyubiquitin-B monomer, 34 kDa Homo sapiens protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.2 nm
Dmax 12.2 nm
VolumePorod 43 nm3

SASDRN7 – M1-linked tetraubiquitin Pro-Ser/Gly-Ser fusion, M1(1-76)-PS(GS)4-Ub4

UniProt ID: None (1-76) Tetraubiquitin, M1(1-76)-PS(GS)4-Ub4
Tetraubiquitin, M1(1-76)-PS(GS)4-Ub4 experimental SAS data
Tetraubiquitin, M1(1-76)-PS(GS)4-Ub4 Kratky plot
Sample: Tetraubiquitin, M1(1-76)-PS(GS)4-Ub4 monomer, 36 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 3.4 nm
Dmax 13.9 nm
VolumePorod 50 nm3

SASDRP7 – M1-linked tetraubiquitin Glu-Ala-Lys fusion, M1(1-74)-A(EA3K)3A-Ub4

UniProt ID: None (1-74) Tetraubiquitin, M1(1-74)-A(EA3K)3A-Ub
Tetraubiquitin, M1(1-74)-A(EA3K)3A-Ub experimental SAS data
Tetraubiquitin, M1(1-74)-A(EA3K)3A-Ub Kratky plot
Sample: Tetraubiquitin, M1(1-74)-A(EA3K)3A-Ub monomer, 39 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 4.2 nm
Dmax 18.2 nm
VolumePorod 62 nm3

SASDRQ7 – M1-linked tetraubiquitin Glu-Ala-Lys fusion, M1(1-74)-A(EA3K)6A-Ub4

UniProt ID: None (1-74) Tetraubiquitin, M1(1-74)-A(EA3K)6A-Ub
Tetraubiquitin, M1(1-74)-A(EA3K)6A-Ub experimental SAS data
Tetraubiquitin, M1(1-74)-A(EA3K)6A-Ub Kratky plot
Sample: Tetraubiquitin, M1(1-74)-A(EA3K)6A-Ub monomer, 43 kDa synthetic construct protein
Buffer: 20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Nov 28
Polyubiquitin ligand-induced phase transitions are optimized by spacing between ubiquitin units Proceedings of the National Academy of Sciences 120(42) (2023)
Galagedera S, Dao T, Enos S, Chaudhuri A, Schmit J, Castañeda C
RgGuinier 5.2 nm
Dmax 21.2 nm
VolumePorod 108 nm3

SASDRZ7 – Human wild-type nicotinamide phosphoribosyltransferase (NAMPT)

UniProt ID: P43490 (2-491) Nicotinamide phosphoribosyltransferase
Nicotinamide phosphoribosyltransferase experimental SAS data
Nicotinamide phosphoribosyltransferase Kratky plot
Sample: Nicotinamide phosphoribosyltransferase dimer, 114 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 20
Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity. J Struct Biol :108004 (2023)
Houry D, Raasakka A, Ferrario E, Niere M, Bifulco E, Kursula P, Ziegler M
RgGuinier 3.3 nm
Dmax 12.9 nm
VolumePorod 181 nm3

SASDR28 – Human wild-type nicotinamide phosphoribosyltransferase (NAMPT) in the presence of nicotinamide

UniProt ID: P43490 (2-491) Nicotinamide phosphoribosyltransferase
Nicotinamide phosphoribosyltransferase experimental SAS data
Nicotinamide phosphoribosyltransferase Kratky plot
Sample: Nicotinamide phosphoribosyltransferase dimer, 114 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM nicotinamide, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 20
Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity. J Struct Biol :108004 (2023)
Houry D, Raasakka A, Ferrario E, Niere M, Bifulco E, Kursula P, Ziegler M
RgGuinier 3.2 nm
Dmax 12.8 nm
VolumePorod 166 nm3

SASDR38 – Human wild-type nicotinamide phosphoribosyltransferase (NAMPT) in the presence of nicotinic acid

UniProt ID: P43490 (2-491) Nicotinamide phosphoribosyltransferase
Nicotinamide phosphoribosyltransferase experimental SAS data
Nicotinamide phosphoribosyltransferase Kratky plot
Sample: Nicotinamide phosphoribosyltransferase dimer, 114 kDa Homo sapiens protein
Buffer: 20 mM Tris-HCl, 500 mM NaCl, 6 mM MgCl2, 5% (v/v) glycerol, 1 mM nicotinic acid, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2019 May 20
Identification of structural determinants of nicotinamide phosphoribosyl transferase (NAMPT) activity and substrate selectivity. J Struct Biol :108004 (2023)
Houry D, Raasakka A, Ferrario E, Niere M, Bifulco E, Kursula P, Ziegler M
RgGuinier 3.3 nm
Dmax 12.9 nm
VolumePorod 180 nm3