SASBDB entries for UniProt ID:

SASDUD4 – Xylanase Updated Consensus SAXS Data

UniProt ID: F8W669 (1-190) Endo-1,4-beta-xylanase
Endo-1,4-beta-xylanase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Endo-1,4-beta-xylanase monomer, 21 kDa Trichoderma longibrachiatum protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
Trewhella J, Vachette P, Larsen A
RgGuinier 1.6 nm
Dmax 5.2 nm

SASDUE4 – Lysozyme Updated Consensus SAXS Data

UniProt ID: P00698 (19-147) Lysozyme C
Lysozyme C experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Lysozyme C monomer, 14 kDa Gallus gallus protein
Buffer: 50 mM sodium citrate, 150 mM NaCl, pH: 4.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
Trewhella J, Vachette P, Larsen A
RgGuinier 1.5 nm
Dmax 4.7 nm

SASDUL4 – Signal transducer and activator of transcription 1-alpha/beta (STAT1)

UniProt ID: P42224 (1-750) Signal transducer and activator of transcription 1-alpha/beta
Signal transducer and activator of transcription 1-alpha/beta experimental SAS data
Signal transducer and activator of transcription 1-alpha/beta Kratky plot
Sample: Signal transducer and activator of transcription 1-alpha/beta tetramer, 349 kDa Homo sapiens protein
Buffer: 10 mM HEPES-NaOH, 150 mM NaCl, 3% glycerol, 2 mM DTT, pH: 7.4
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2022 Dec 11
Structural analysis reveals how tetrameric tyrosine-phosphorylated STAT1 is targeted by the rabies virus P-protein Science Signaling 18(878) (2025)
Sugiyama A, Minami M, Ugajin K, Inaba-Inoue S, Yabuno N, Takekawa Y, Xiaomei S, Takei S, Sasaki M, Nomai T, Jiang X, Kita S, Maenaka K, Hirose M, Yao M, Gooley P, Moseley G, Sugita Y, Ose T
RgGuinier 6.2 nm
Dmax 21.2 nm
VolumePorod 800 nm3

SASDUM4 – Signal transducer and activator of transcription 1-alpha/beta bound to phosphoprotein (STAT1/RVP)

UniProt ID: P42224 (1-750) Signal transducer and activator of transcription 1-alpha/beta

UniProt ID: Q9IPJ8 (1-297) Phosphoprotein
Signal transducer and activator of transcription 1-alpha/betaPhosphoprotein experimental SAS data
Signal transducer and activator of transcription 1-alpha/beta Phosphoprotein Kratky plot
Sample: Signal transducer and activator of transcription 1-alpha/beta tetramer, 349 kDa Homo sapiens protein
Phosphoprotein monomer, 33 kDa Rabies virus (strain … protein
Buffer: 10 mM HEPES-NaOH, 150 mM NaCl, 3% glycerol, 2 mM DTT, pH: 7.4
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2022 Dec 11
Structural analysis reveals how tetrameric tyrosine-phosphorylated STAT1 is targeted by the rabies virus P-protein Science Signaling 18(878) (2025)
Sugiyama A, Minami M, Ugajin K, Inaba-Inoue S, Yabuno N, Takekawa Y, Xiaomei S, Takei S, Sasaki M, Nomai T, Jiang X, Kita S, Maenaka K, Hirose M, Yao M, Gooley P, Moseley G, Sugita Y, Ose T
RgGuinier 6.5 nm
Dmax 26.0 nm
VolumePorod 857 nm3

SASDUN4 – Signal transducer and activator of transcription 1-alpha/beta bound to transducer/activator DNA (STAT1/DNA)

UniProt ID: P42224 (1-750) Signal transducer and activator of transcription 1-alpha/beta

UniProt ID: None (None-None) Signal transducer and activator of transcription 1 binding DNA
Signal transducer and activator of transcription 1-alpha/betaSignal transducer and activator of transcription 1 binding DNA experimental SAS data
Signal transducer and activator of transcription 1-alpha/beta Signal transducer and activator of transcription 1 binding DNA Kratky plot
Sample: Signal transducer and activator of transcription 1-alpha/beta tetramer, 349 kDa Homo sapiens protein
Signal transducer and activator of transcription 1 binding DNA dimer, 11 kDa Homo sapiens DNA
Buffer: 10 mM HEPES-NaOH, 150 mM NaCl, 3% glycerol, 2 mM DTT, pH: 7.4
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2022 Dec 11
Structural analysis reveals how tetrameric tyrosine-phosphorylated STAT1 is targeted by the rabies virus P-protein Science Signaling 18(878) (2025)
Sugiyama A, Minami M, Ugajin K, Inaba-Inoue S, Yabuno N, Takekawa Y, Xiaomei S, Takei S, Sasaki M, Nomai T, Jiang X, Kita S, Maenaka K, Hirose M, Yao M, Gooley P, Moseley G, Sugita Y, Ose T
RgGuinier 6.1 nm
Dmax 19.4 nm
VolumePorod 761 nm3

SASDUQ4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain

UniProt ID: Q9UQM7 (345-475) Calcium/calmodulin-dependent protein kinase type II subunit alpha
Calcium/calmodulin-dependent protein kinase type II subunit alpha experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment: SAXS data collected at EMBL P12, PETRA III on 2022 Oct 3
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.5 nm
Dmax 15.2 nm
VolumePorod 367 nm3

SASDUR4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain

UniProt ID: Q9UQM7 (345-475) Calcium/calmodulin-dependent protein kinase type II subunit alpha
Calcium/calmodulin-dependent protein kinase type II subunit alpha experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2020 Nov 19
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.4 nm
Dmax 14.9 nm
VolumePorod 351 nm3

SASDUS4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (i)

UniProt ID: Q9UQM7 (345-475) Calcium/calmodulin-dependent protein kinase type II subunit alpha

UniProt ID: None (None-None) 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid
Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2022 Nov 30
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 4.8 nm
Dmax 16.0 nm
VolumePorod 396 nm3

SASDUT4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (ii)

UniProt ID: Q9UQM7 (345-475) Calcium/calmodulin-dependent protein kinase type II subunit alpha

UniProt ID: None (None-None) 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid
Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2023 May 30
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 5.5 nm
Dmax 27.0 nm

SASDUU4 – Calcium/calmodulin-dependent protein kinase type II alpha (CaMKIIα) WT hub domain with PIPA (ii-remeasured after storage)

UniProt ID: Q9UQM7 (345-475) Calcium/calmodulin-dependent protein kinase type II subunit alpha

UniProt ID: None (None-None) 2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid
Calcium/calmodulin-dependent protein kinase type II subunit alpha2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid experimental SAS data
PYMOL model
Sample: Calcium/calmodulin-dependent protein kinase type II subunit alpha dodecamer, 186 kDa Homo sapiens protein
2-(6-(4-chlorophenyl)imidazo[1,2-b]pyridazine-2-yl)acetic acid monomer, 0 kDa
Buffer: 20 mM MES, 150 mM NaCl, 1 mM DTT, 200 µM PIPA, pH: 6
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2023 May 31
Ligand‐induced CaMKIIα hub Trp403 flip, hub domain stacking, and modulation of kinase activity Protein Science 33(10) (2024)
Narayanan D, Larsen A, Gauger S, Adafia R, Hammershøi R, Hamborg L, Bruus‐Jensen J, Griem‐Krey N, Gee C, Frølund B, Stratton M, Kuriyan J, Kastrup J, Langkilde A, Wellendorph P, Solbak S
RgGuinier 8.8 nm
Dmax 37.0 nm