SASBDB entries for UniProt ID:

SASDUJ3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) deltaX283

UniProt ID: N4UN01 (21-277) Endoglucanase-7

Endoglucanase-7 experimental SAS data
Endoglucanase-7 Kratky plot
Sample: Endoglucanase-7 monomer, 27 kDa Colletotrichum orbiculare (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Feb 1
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 1.8 nm
Dmax 6.3 nm
VolumePorod 32 nm3

SASDUK3 – Colletotrichum orbiculare Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) C286A*

UniProt ID: N4UN01 (21-303) Endoglucanase-7 (C286A)

Endoglucanase-7 (C286A) experimental SAS data
Endoglucanase-7 (C286A) Kratky plot
Sample: Endoglucanase-7 (C286A) monomer, 30 kDa Colletotrichum orbiculare (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2023 Feb 1
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 3.0 nm
Dmax 11.6 nm
VolumePorod 48 nm3

SASDUL3 – Colletotrichum higginsianum Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) monomer

UniProt ID: H1VPG7 (21-312) Glycosyl hydrolase family 61

Glycosyl hydrolase family 61 experimental SAS data
Glycosyl hydrolase family 61 Kratky plot
Sample: Glycosyl hydrolase family 61 monomer, 30 kDa Colletotrichum higginsianum (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 9
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 4.1 nm
Dmax 14.6 nm
VolumePorod 85 nm3

SASDUM3 – Colletotrichum higginsianum Auxiliary Activity Family 9A Lytic Polysaccharide Monooxygenase (AA9A LPMO) dimer

UniProt ID: H1VPG7 (21-312) Glycosyl hydrolase family 61

Glycosyl hydrolase family 61 experimental SAS data
Glycosyl hydrolase family 61 Kratky plot
Sample: Glycosyl hydrolase family 61 dimer, 61 kDa Colletotrichum higginsianum (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at BM29, ESRF on 2022 Jun 9
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 6.2 nm
Dmax 24.5 nm
VolumePorod 206 nm3

SASDUN3 – Colletotrichum higginsianum Auxiliary Activity Family 9B Lytic Polysaccharide Monooxygenase (AA9B LPMO) monomer

UniProt ID: A0A1B7Y5F6 (22-335) Endoglucanase-4

Endoglucanase-4 experimental SAS data
Endoglucanase-4 Kratky plot
Sample: Endoglucanase-4 monomer, 33 kDa Colletotrichum higginsianum (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Feb 17
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 3.9 nm
Dmax 17.0 nm
VolumePorod 68 nm3

SASDUP3 – Colletotrichum higginsianum Auxiliary Activity Family 9B Lytic Polysaccharide Monooxygenase (AA9B LPMO) dimer

UniProt ID: A0A1B7Y5F6 (22-335) Endoglucanase-4

Endoglucanase-4 experimental SAS data
Endoglucanase-4 Kratky plot
Sample: Endoglucanase-4 dimer, 66 kDa Colletotrichum higginsianum (strain … protein
Buffer: 50 mM MES, 150 mM NaCl, pH: 6.5
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Feb 17
The disordered C-terminal tail of fungal LPMOs from phytopathogens mediates protein dimerization and impacts plant penetration. Proc Natl Acad Sci U S A 121(13):e2319998121 (2024)
Tamburrini KC, Kodama S, Grisel S, Haon M, Nishiuchi T, Bissaro B, Kubo Y, Longhi S, Berrin JG
RgGuinier 6.4 nm
Dmax 27.9 nm
VolumePorod 199 nm3

SASDUQ3 – C-terminal domain of the W protein (CTDw) of Hendra Virus (HeV)

UniProt ID: P0C1C6 (405-448) Protein W

Protein W experimental SAS data
C-terminal domain of the W protein (CTDw) of Hendra Virus (HeV) Rg histogram
Sample: Protein W monomer, 6 kDa Hendra virus (isolate … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 16
Dissecting Henipavirus W proteins conformational and fibrillation properties: contribution of their N- and C-terminal constituent domains. FEBS J (2024)
Pesce G, Gondelaud F, Ptchelkine D, Bignon C, Fourquet P, Longhi S
RgGuinier 2.1 nm
Dmax 7.4 nm
VolumePorod 8 nm3

SASDUR3 – C-terminal domain of the W protein (CTDw) of Nipah Virus (NiV)

UniProt ID: P0C1C7 (407-450) Protein W

Protein W experimental SAS data
C-terminal domain of the W protein (CTDw) of Nipah Virus (NiV) Rg histogram
Sample: Protein W monomer, 6 kDa Henipavirus nipahense protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2023 Apr 16
Dissecting Henipavirus W proteins conformational and fibrillation properties: contribution of their N- and C-terminal constituent domains. FEBS J (2024)
Pesce G, Gondelaud F, Ptchelkine D, Bignon C, Fourquet P, Longhi S
RgGuinier 2.1 nm
Dmax 7.8 nm
VolumePorod 8 nm3

SASDUS3 – Dark-adapted photocobilin diguanylate cyclase fusion from Acidimicrobiaceae bacterium

UniProt ID: A0A349B205 (1-608) GGDEF domain-containing protein

GGDEF domain-containing protein experimental SAS data
DAMMIF model
Sample: GGDEF domain-containing protein dimer, 132 kDa Acidimicrobiaceae bacterium protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 6.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jan 21
Photocobilins integrate B(12) and bilin photochemistry for enzyme control. Nat Commun 15(1):2740 (2024)
Zhang S, Jeffreys LN, Poddar H, Yu Y, Liu C, Patel K, Johannissen LO, Zhu L, Cliff MJ, Yan C, Schirò G, Weik M, Sakuma M, Levy CW, Leys D, Heyes DJ, Scrutton NS
RgGuinier 4.1 nm
Dmax 14.9 nm
VolumePorod 194 nm3

SASDUT3 – Light-adapted photocobilin diguanylate cyclase fusion from Acidimicrobiaceae bacterium

UniProt ID: A0A349B205 (1-608) GGDEF domain-containing protein

GGDEF domain-containing protein experimental SAS data
DAMMIF model
Sample: GGDEF domain-containing protein dimer, 132 kDa Acidimicrobiaceae bacterium protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 6.8
Experiment: SAXS data collected at BM29, ESRF on 2022 Jan 21
Photocobilins integrate B(12) and bilin photochemistry for enzyme control. Nat Commun 15(1):2740 (2024)
Zhang S, Jeffreys LN, Poddar H, Yu Y, Liu C, Patel K, Johannissen LO, Zhu L, Cliff MJ, Yan C, Schirò G, Weik M, Sakuma M, Levy CW, Leys D, Heyes DJ, Scrutton NS
RgGuinier 5.0 nm
Dmax 18.5 nm
VolumePorod 170 nm3