SASBDB entries for UniProt ID:

SASDU34 – Calcium activated full-length gelsolin and the F-form of actin at a 1:4 molar ratio in F-actin buffer

UniProt ID: P06396 (25-782) Gelsolin

UniProt ID: P60706 (1-375) Actin, cytoplasmic 1
GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 8.8 nm

SASDU44 – Calcium activated full-length gelsolin and the F-form of actin at a 1:2 molar ratio in F-actin buffer

UniProt ID: P06396 (25-782) Gelsolin

UniProt ID: P60706 (1-375) Actin, cytoplasmic 1
GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 7.3 nm

SASDU54 – Calcium activated full-length gelsolin and the F-form of actin at a 1:1 molar ratio in F-actin buffer

UniProt ID: P06396 (25-782) Gelsolin

UniProt ID: P60706 (1-375) Actin, cytoplasmic 1
GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 18-mer, 751 kDa Gallus gallus protein
Buffer: 50 mM KCl, 50 mM Tris-HCl, pH 8.0, 5 mM MgCl2, 1 mM ATP, 0.1% 2-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 5.2 nm

SASDU64 – Calcium-gelsolin and the F-form of actin at a 1:2 molar ratio from high to low ionic strength

UniProt ID: P06396 (25-782) Gelsolin

UniProt ID: P60706 (1-375) Actin, cytoplasmic 1
GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein
Buffer: 2 mM Tris-Cl, pH 8.0, 0.2 mM ATP, 1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 4.7 nm
Dmax 25.0 nm
VolumePorod 262 nm3

SASDU74 – Calcium-gelsolin and the F-form of actin at a 1:1 molar ratio from high to low ionic strength

UniProt ID: P06396 (25-782) Gelsolin

UniProt ID: P60706 (1-375) Actin, cytoplasmic 1
GelsolinActin, cytoplasmic 1 experimental SAS data
Gelsolin Actin, cytoplasmic 1 Kratky plot
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein
Buffer: 2 mM Tris-Cl, pH 8.0, 0.2 mM ATP, 1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 5.2 nm
Dmax 25.0 nm
VolumePorod 266 nm3

SASDU84 – Calcium-gelsolin and the G-form of actin at a 1:2 molar ratio in low ionic strength (SEC-SAXS)

UniProt ID: P06396 (25-782) Gelsolin

UniProt ID: P60706 (1-375) Actin, cytoplasmic 1
GelsolinActin, cytoplasmic 1 experimental SAS data
GASBOR model
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein
Buffer: 2 mM Tris-Cl, pH 8.0, 0.2 mM ATP, 1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Aug 31
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 4.4 nm
Dmax 25.0 nm
VolumePorod 241 nm3

SASDU94 – Calcium-gelsolin and the G-form of actin at a 1:1 molar ratio in low ionic strength (SEC-SAXS)

UniProt ID: P06396 (25-782) Gelsolin

UniProt ID: P60706 (1-375) Actin, cytoplasmic 1
GelsolinActin, cytoplasmic 1 experimental SAS data
GASBOR model
Sample: Gelsolin monomer, 84 kDa Homo sapiens protein
Actin, cytoplasmic 1 monomer, 42 kDa Gallus gallus protein
Buffer: 2 mM Tris-Cl, pH 8.0, 0.2 mM ATP, 1 mM NaN3, 0.1 mM CaCl2, 0.5 mM DTT, pH: 8
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Sep 1
Visualizing the nucleating and capped states of f-actin by Ca(2+)-gelsolin: Saxs data based structures of binary and ternary complexes. Int J Biol Macromol :134556 (2024)
Sagar A, Peddada N, Choudhary V, Mir Y, Garg R, Ashish
RgGuinier 4.6 nm
Dmax 25.0 nm
VolumePorod 243 nm3

SASDUA4 – Ribonuclease A Updated Consensus SAXS Data

UniProt ID: P61823 (27-150) Ribonuclease pancreatic
Ribonuclease pancreatic experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Ribonuclease pancreatic monomer, 14 kDa Bos taurus protein
Buffer: 50 mM Tris, 100 mM NaCl, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
Trewhella J, Vachette P, Larsen A
RgGuinier 1.5 nm
Dmax 5.0 nm

SASDUB4 – Urate Oxidase Updated Consensus SAXS Data

UniProt ID: Q00511 (2-302) Uricase
Uricase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Uricase tetramer, 136 kDa Aspergillus flavus protein
Buffer: 100 mM Tris, 150 mM NaCl, pH: 8
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
Trewhella J, Vachette P, Larsen A
RgGuinier 3.2 nm
Dmax 9.3 nm

SASDUC4 – Xylose Isomerase Updated Consensus SAXS Data

UniProt ID: P24300 (1-388) Xylose isomerase
Xylose isomerase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Xylose isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer: 50 mM Tris, 100 mM NaCl, 1 mM MgCl2, pH: 7.5
Experiment: SANS data collected at (Consensus SAS), Multi-facility, Multiple countries on 2024 Feb 4
Benchmarking predictive methods for small-angle X-ray scattering from atomic coordinates of proteins using maximum likelihood consensus data IUCrJ 11(5) (2024)
Trewhella J, Vachette P, Larsen A
RgGuinier 3.3 nm
Dmax 10.1 nm