SASBDB entries for UniProt ID:

SASDDY9 – Protein translocase subunit SecA (full length, amino acids 1-901)

UniProt ID: P10408 (None-None) Protein translocase subunit SecA
Protein translocase subunit SecA experimental SAS data
Protein translocase subunit SecA Kratky plot
Sample: Protein translocase subunit SecA dimer, 204 kDa Escherichia coli protein
Buffer: 20mM HEPES, 100mM NaCl, 1mM TCEP, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 18
The C-terminal tail of the bacterial translocation ATPase SecA modulates its activity. Elife 8 (2019)
Jamshad M, Knowles TJ, White SA, Ward DG, Mohammed F, Rahman KF, Wynne M, Hughes GW, Kramer G, Bukau B, Huber D
RgGuinier 4.2 nm
Dmax 14.9 nm
VolumePorod 424 nm3

SASDEP3 – Senescence-associated E3 ubiquitin ligase 1, 7.3 mg/ml

UniProt ID: Q9LM76 (None-None) Senescence-associated E3 ubiquitin ligase 1
Senescence-associated E3 ubiquitin ligase 1 experimental SAS data
SASREF MX model
Sample: Senescence-associated E3 ubiquitin ligase 1 tetramer, 355 kDa Arabidopsis thaliana protein
Buffer: 50 mM Tris, 250 mM NaCl, pH: 9
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Jun 2
Senescence-associated ubiquitin ligase 1 (SAUL1)
Haifa El Kilani, Al Kikhney
RgGuinier 6.5 nm
Dmax 24.0 nm

SASDEK4 – C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein (ScsC)

UniProt ID: A0A1Z1SYD5 (64-243) C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein
C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein experimental SAS data
DAMFILT model
Sample: C-terminal catalytic domain of Suppressor of Copper Sensitivity C protein monomer, 20 kDa Proteus mirabilis protein
Buffer: 10mM HEPES, 150mM NaCl, pH: 7.4
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2018 Apr 5
Engineered variants provide new insight into the structural properties important for activity of the highly dynamic, trimeric protein disulfide isomerase ScsC from Proteus mirabilis. Acta Crystallogr D Struct Biol 75(Pt 3):296-307 (2019)
Furlong EJ, Kurth F, Premkumar L, Whitten AE, Martin JL
RgGuinier 1.7 nm
Dmax 5.4 nm
VolumePorod 22200 nm3

SASDEU5 – Adenylation domain of DNA ligase A

UniProt ID: P9WNV1 (2-328) DNA ligase A
DNA ligase A experimental SAS data
DAMFILT model
Sample: DNA ligase A monomer, 38 kDa Mycobacterium tuberculosis protein
Buffer: 50 mM Tris-HCl, 200 mM NaCl, 2 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at Anton Paar SAXSpace, CSIR-Central Drug Research Institute on 2018 Sep 27
Salt bridges at the subdomain interfaces of the adenylation domain and active-site residues of Mycobacterium tuberculosis NAD + -dependent DNA ligase A (MtbLigA) are important for the initial steps of nick-sealing activity Acta Crystallographica Section D Structural Biology 77(6) (2021)
Afsar M, Shukla A, Kumar N, Ramachandran R
RgGuinier 2.4 nm
Dmax 8.8 nm
VolumePorod 69 nm3

SASDEV5 – Insulin detemir tri-hexamer

UniProt ID: P01308 (25-110) Insulin detemir
Insulin detemir experimental SAS data
SASREF CV model
Sample: Insulin detemir 18-mer, 106 kDa protein
Buffer: 5.0 mM Na2HPO4, 13.1 mM m-cresol, 15.1 mM phenol, 173.7 mM glycerol, 20.0 mM NaCl, pH: 7.4
Experiment: SAXS data collected at I911-4, MAX IV on 2015 Sep 25
Solution structures of long-acting insulin analogues and their complexes with albumin. Acta Crystallogr D Struct Biol 75(Pt 3):272-282 (2019)
Ryberg LA, Sønderby P, Barrientos F, Bukrinski JT, Peters GHJ, Harris P
RgGuinier 3.3 nm
Dmax 11.3 nm
VolumePorod 137 nm3

SASDEV6 – KRAB-associated protein 1 (KAP1); TRIM28; full length protein

UniProt ID: Q13263 (1-835) Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28
Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 experimental SAS data
GASBOR model
Sample: Transcription intermediary factor 1-beta, TIF1b, KAP1, TRIM28 dimer, 183 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 500 mM NaCl, 10 % Glycerol, 2 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 6
KAP1 is an antiparallel dimer with a functional asymmetry. Life Sci Alliance 2(4) (2019)
Fonti G, Marcaida MJ, Bryan LC, Träger S, Kalantzi AS, Helleboid PJ, Demurtas D, Tully MD, Grudinin S, Trono D, Fierz B, Dal Peraro M
RgGuinier 9.0 nm
Dmax 38.0 nm
VolumePorod 710 nm3

SASDEQ7 – Filamin A Ig-like domains 3-5 (FLNa3-5)

UniProt ID: P21333 (478-766) Filamin A Ig-like domains 3-5
Filamin A Ig-like domains 3-5 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Filamin A Ig-like domains 3-5 monomer, 31 kDa Homo sapiens protein
Buffer: 20 mM Tris, 100 mM NaCl, 1 mM DTT, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 Feb 10
Non-syndromic Mitral Valve Dysplasia Mutation Changes the Force Resilience and Interaction of Human Filamin A. Structure (2018)
Haataja TJK, Bernardi RC, Lecointe S, Capoulade R, Merot J, Pentikäinen U
RgGuinier 2.2 nm
Dmax 7.3 nm
VolumePorod 39 nm3

SASDEU7 – Stator protein FlaG soluble domain

UniProt ID: Q4J9K7 (32-151) Conserved flagellar protein FlaG soluble domain
Conserved flagellar protein FlaG soluble domain experimental SAS data
Conserved flagellar protein FlaG soluble domain Kratky plot
Sample: Conserved flagellar protein FlaG soluble domain monomer, 15 kDa Sulfolobus acidocaldarius protein
Buffer: 25 mM citric acid/sodium citrate, 150mM NaCl, 3% Glycerol, pH: 3
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 10
The structure of the periplasmic FlaG-FlaF complex and its essential role for archaellar swimming motility. Nat Microbiol (2019)
Tsai CL, Tripp P, Sivabalasarma S, Zhang C, Rodriguez-Franco M, Wipfler RL, Chaudhury P, Banerjee A, Beeby M, Whitaker RJ, Tainer JA, Albers SV
RgGuinier 3.7 nm
Dmax 18.0 nm
VolumePorod 133 nm3

SASDEW8 – Shwachman-Bodian-Diamond Syndrome protein (SDO1) with domains 2 and 3

UniProt ID: Q07953 (1-250) Ribosome maturation protein SDO1
Ribosome maturation protein SDO1 experimental SAS data
DAMFILT model
Sample: Ribosome maturation protein SDO1 monomer, 17 kDa Saccharomyces cerevisiae protein
Buffer: 50 mM Tris pH 8.0, 10% glycerol, 300 mM NaCl, 5 mM MgCl2., pH:
Experiment: SAXS data collected at EMBL P12, PETRA III on 2015 Aug 14
Interaction of the GTPase Elongation Factor Like-1 with the Shwachman-Diamond Syndrome Protein and Its Missense Mutations. Int J Mol Sci 19(12) (2018)
Gijsbers A, Montagut DC, Méndez-Godoy A, Altamura D, Saviano M, Siliqi D, Sánchez-Puig N
RgGuinier 2.1 nm
Dmax 6.2 nm
VolumePorod 25 nm3

SASDE59 – Interleukin-1 receptor type 1, IL-1RI-ECD

UniProt ID: P14778 (18-331) Interleukin-1 receptor type 1
Interleukin-1 receptor type 1 experimental SAS data
BILBOMD model
Sample: Interleukin-1 receptor type 1 monomer, 37 kDa Homo sapiens protein
Buffer: 10mM HEPES, 150mM NaCl, 3% glycerol, pH: 7.2
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2017 Jul 22
Functional Relevance of Interleukin-1 Receptor Inter-domain Flexibility for Cytokine Binding and Signaling. Structure 27(8):1296-1307.e5 (2019)
Ge J, Remesh SG, Hammel M, Pan S, Mahan AD, Wang S, Wang X
RgGuinier 3.0 nm
Dmax 10.5 nm
VolumePorod 63 nm3