SASBDB entries for UniProt ID:

SASDEN2 – Labeled Nuclear Localization Signal from the inner nuclear membrane protein HEH2 (NLS-Alexa488/Alexa594) without denaturant

UniProt ID: Q03281 (99-140) Inner nuclear membrane protein HEH2

UniProt ID: None (None-None) Alexa Fluor™ 594 C5 Maleimide

UniProt ID: None (None-None) Alexa Fluor™ 488 C5 Hydroxylamine
Inner nuclear membrane protein HEH2Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Inner nuclear membrane protein HEH2 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Inner nuclear membrane protein HEH2 monomer, 5 kDa Saccharomyces cerevisiae protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: 25 mM HEPES, 150 mM NaCl, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 25
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 2.0 nm
Dmax 7.3 nm
VolumePorod 10 nm3

SASDEP2 – Unlabeled Nuclear Localization Signal (NLS) from the inner nuclear membrane protein HEH2 with denaturant

UniProt ID: Q03281 (99-140) Inner nuclear membrane protein HEH2
Inner nuclear membrane protein HEH2 experimental SAS data
Unlabeled Nuclear Localization Signal (NLS) from the inner nuclear membrane protein HEH2 with denaturant Rg histogram
Sample: Inner nuclear membrane protein HEH2 monomer, 5 kDa Saccharomyces cerevisiae protein
Buffer: 25 mM HEPES, 150 mM NaCl, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 25
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 2.3 nm
Dmax 7.1 nm
VolumePorod 10 nm3

SASDEQ2 – Labeled Nuclear Localization Signal from the inner nuclear membrane protein HEH2 (NLS-Alexa488/Alexa594) with denaturant

UniProt ID: Q03281 (99-140) Inner nuclear membrane protein HEH2

UniProt ID: None (None-None) Alexa Fluor™ 594 C5 Maleimide

UniProt ID: None (None-None) Alexa Fluor™ 488 C5 Hydroxylamine
Inner nuclear membrane protein HEH2Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Inner nuclear membrane protein HEH2 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Inner nuclear membrane protein HEH2 monomer, 5 kDa Saccharomyces cerevisiae protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: 25 mM HEPES, 150 mM NaCl, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2014 Jan 25
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 2.4 nm
Dmax 8.1 nm
VolumePorod 12 nm3

SASDER2 – Unlabeled Importin Beta Binding domain (IBB) from importin subunit alpha-1 without denaturant

UniProt ID: P52292 (3-97) Importin subunit alpha-1
Importin subunit alpha-1 experimental SAS data
Unlabeled Importin Beta Binding domain (IBB) from importin subunit alpha-1 without denaturant Rg histogram
Sample: Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 3.2 nm
Dmax 11.5 nm
VolumePorod 30 nm3

SASDES2 – Labeled Importin Beta Binding Domain (IBB-Alexa488/Alexa594) from importin subunit alpha-1 without denaturant

UniProt ID: P52292 (3-97) Importin subunit alpha-1

UniProt ID: None (None-None) Alexa Fluor™ 594 C5 Maleimide

UniProt ID: None (None-None) Alexa Fluor™ 488 C5 Hydroxylamine
Importin subunit alpha-1Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Importin subunit alpha-1 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Dec 9
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 2.9 nm
Dmax 13.7 nm
VolumePorod 29 nm3

SASDET2 – Unlabeled Importin Beta Binding Domain (IBB) from importin subunit alpha-1 with denaturant

UniProt ID: P52292 (3-97) Importin subunit alpha-1
Importin subunit alpha-1 experimental SAS data
Unlabeled Importin Beta Binding Domain (IBB) from importin subunit alpha-1 with denaturant Rg histogram
Sample: Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 3.1 nm
Dmax 15.3 nm
VolumePorod 34 nm3

SASDEU2 – Labeled Importin Beta Binding Domain from importin subunit alpha-1 (IBB-Alexa488/Alexa594) with denaturant

UniProt ID: P52292 (3-97) Importin subunit alpha-1

UniProt ID: None (None-None) Alexa Fluor™ 594 C5 Maleimide

UniProt ID: None (None-None) Alexa Fluor™ 488 C5 Hydroxylamine
Importin subunit alpha-1Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Importin subunit alpha-1 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Importin subunit alpha-1 monomer, 11 kDa Homo sapiens protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 3.2 nm
Dmax 13.3 nm
VolumePorod 29 nm3

SASDEV2 – Unlabeled nuclear pore complex protein Nup153 (NUS) without denaturant

UniProt ID: P49790 (1313-1390) Nuclear pore complex protein Nup153
Nuclear pore complex protein Nup153 experimental SAS data
Unlabeled nuclear pore complex protein Nup153 (NUS) without denaturant Rg histogram
Sample: Nuclear pore complex protein Nup153 monomer, 8 kDa Homo sapiens protein
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 2.5 nm
Dmax 9.2 nm
VolumePorod 15 nm3

SASDEW2 – Labeled nuclear pore complex protein Nup153 (NUS-Alexa488/Alexa594) without denaturant

UniProt ID: P49790 (1313-1390) Nuclear pore complex protein Nup153

UniProt ID: None (None-None) Alexa Fluor™ 594 C5 Maleimide

UniProt ID: None (None-None) Alexa Fluor™ 488 C5 Hydroxylamine
Nuclear pore complex protein Nup153Alexa Fluor™ 594 C5 MaleimideAlexa Fluor™ 488 C5 Hydroxylamine experimental SAS data
Nuclear pore complex protein Nup153 Alexa Fluor™ 594 C5 Maleimide Alexa Fluor™ 488 C5 Hydroxylamine Kratky plot
Sample: Nuclear pore complex protein Nup153 monomer, 8 kDa Homo sapiens protein
Alexa Fluor™ 594 C5 Maleimide monomer, 1 kDa
Alexa Fluor™ 488 C5 Hydroxylamine monomer, 1 kDa
Buffer: PBS, 10 mM DTT, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Nov 8
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 2.5 nm
Dmax 9.7 nm
VolumePorod 17 nm3

SASDEX2 – Unlabeled nuclear pore complex protein Nup153 (NUS) with denaturant

UniProt ID: P49790 (1313-1390) Nuclear pore complex protein Nup153
Nuclear pore complex protein Nup153 experimental SAS data
Unlabeled nuclear pore complex protein Nup153 (NUS) with denaturant Rg histogram
Sample: Nuclear pore complex protein Nup153 monomer, 8 kDa Homo sapiens protein
Buffer: PBS, 10 mM DTT, 6 M urea, 0.3 M KCl, pH: 7.4
Experiment: SAXS data collected at EMBL P12, PETRA III on 2013 Jun 15
Decoupling of size and shape fluctuations in heteropolymeric sequences reconciles discrepancies in SAXS vs. FRET measurements. Proc Natl Acad Sci U S A 114(31):E6342-E6351 (2017)
Fuertes G, Banterle N, Ruff KM, Chowdhury A, Mercadante D, Koehler C, Kachala M, Estrada Girona G, Milles S, Mishra A, Onck PR, Gräter F, Esteban-Martín S, Pappu RV, Svergun DI, Lemke EA
RgGuinier 3.1 nm
Dmax 11.0 nm
VolumePorod 26 nm3