SASBDB entries for UniProt ID:

SASDEJ5 – AICAR/fumarate-bound human adenylosuccinate lyase (ADSL)

UniProt ID: P30566 (1-484) Adenylosuccinate Lyase
Adenylosuccinate Lyase experimental SAS data
Adenylosuccinate Lyase Kratky plot
Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), 1mM Fumarate, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.6 nm
VolumePorod 250 nm3

SASDEK5 – AMP-bound human adenylosuccinate lyase (ADSL)

UniProt ID: P30566 (1-484) Adenylosuccinate Lyase
Adenylosuccinate Lyase experimental SAS data
Adenylosuccinate Lyase Kratky plot
Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM AMP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 292 nm3

SASDEL5 – AICAR-bound human adenylosuccinate lyase (ADSL)

UniProt ID: P30566 (1-484) Adenylosuccinate Lyase
Adenylosuccinate Lyase experimental SAS data
Adenylosuccinate Lyase Kratky plot
Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 291 nm3

SASDEM5 – apo neanderthal adenylosuccinate lyase (ADSL)

UniProt ID: A0A384E0N4 (1-487) Adenylosuccinate lyase
Adenylosuccinate lyase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Nov 13
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 304 nm3

SASDEN5 – AMP/fumarate-bound neanderthal adenylosuccinate lyase (ADSL)

UniProt ID: A0A384E0N4 (1-487) Adenylosuccinate lyase
Adenylosuccinate lyase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1mM AMP, 1mM Fumarate, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 258 nm3

SASDEP5 – AMP-bound neanderthal adenylosuccinate lyase (ADSL)

UniProt ID: A0A384E0N4 (1-487) Adenylosuccinate lyase
Adenylosuccinate lyase experimental SAS data
Adenylosuccinate lyase Kratky plot
Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM AMP, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 294 nm3

SASDEQ5 – AICAR-bound neanderthal adenylosuccinate lyase (ADSL)

UniProt ID: A0A384E0N4 (1-487) Adenylosuccinate lyase
Adenylosuccinate lyase experimental SAS data
Adenylosuccinate lyase Kratky plot
Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 292 nm3

SASDER5 – AICAR/fumarate-bound neanderthal adenylosuccinate lyase (ADSL)

UniProt ID: A0A384E0N4 (1-487) Adenylosuccinate lyase
Adenylosuccinate lyase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Adenylosuccinate lyase tetramer, 221 kDa Homo sapiens neanderthalensis protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1 mM 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR), 1mM Fumarate, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.6 nm
VolumePorod 252 nm3

SASDES5 – Neurotrypsin Scavenger Receptor Cysteine-Rich Domain 3 (mmNT-SRCR3) - from SEC-SAXS

UniProt ID: O08762 (383-494) Mouse Neurotrypsin Scavenger Receptor Cysteine-Rich Domain 3
Mouse Neurotrypsin Scavenger Receptor Cysteine-Rich Domain 3 experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Mouse Neurotrypsin Scavenger Receptor Cysteine-Rich Domain 3 monomer, 13 kDa Mus musculus protein
Buffer: 25 mM HEPES, 0.1 M NaCl, pH: 8
Experiment: SAXS data collected at BM29, ESRF on 2017 May 20
Structural characterization of the third scavenger receptor cysteine-rich domain of murine neurotrypsin. Protein Sci 28(4):746-755 (2019)
Canciani A, Catucci G, Forneris F
RgGuinier 1.5 nm
Dmax 4.5 nm
VolumePorod 24 nm3

SASDE36 – Human ATP-citrate synthase (ACLY) in HBS

UniProt ID: P53396-1 (None-None) ATP-citrate synthase
ATP-citrate synthase experimental SAS data
MULTIFOXS model
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 6
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
Verschueren KHG, Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.0 nm
Dmax 17.5 nm
VolumePorod 738 nm3