SASBDB entries for UniProt ID:

SASDE46 – Human ATP-citrate synthers (ACLY) in HBS + Citrate

UniProt ID: P53396-1 (None-None) ATP-citrate synthase
ATP-citrate synthase experimental SAS data
ATP-citrate synthase Kratky plot
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 6
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
Verschueren KHG, Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 6.1 nm
Dmax 17.5 nm
VolumePorod 747 nm3

SASDE56 – Human ATP-citrate synthase (ACLY) in HBS + Citrate + Coenzyme-A

UniProt ID: P53396-1 (None-None) ATP-citrate synthase
ATP-citrate synthase experimental SAS data
SASREF CV model
Sample: ATP-citrate synthase tetramer, 458 kDa Homo sapiens protein
Buffer: 20mM HEPES, 150mM NaCl, 50mM Tris, 20mM citrate, 2mM CoA, pH: 7.2
Experiment: SAXS data collected at EMBL P12, PETRA III on 2018 May 5
Structure of ATP citrate lyase and the origin of citrate synthase in the Krebs cycle. Nature 568(7753):571-575 (2019)
Verschueren KHG, Blanchet C, Felix J, Dansercoer A, De Vos D, Bloch Y, Van Beeumen J, Svergun D, Gutsche I, Savvides SN, Verstraete K
RgGuinier 5.8 nm
Dmax 16.5 nm
VolumePorod 709 nm3

SASDE96 – Aldehyde dehydrogenase 12 from Zea mays Extrapolated to Infinite Dilution

UniProt ID: A0A2H4PMI3 (17-549) Aldehyde dehydrogenase 12
Aldehyde dehydrogenase 12 experimental SAS data
ALLOSMOD model
Sample: Aldehyde dehydrogenase 12 tetramer, 242 kDa Zea mays protein
Buffer: 50 mM Tris-HCl, 50 mM NaCl, 0.5 mM TCEP, and 5% (v/v) glycerol, pH: 7.8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Dec 6
Structural and Biochemical Characterization of Aldehyde Dehydrogenase 12, the Last Enzyme of Proline Catabolism in Plants. J Mol Biol (2018)
Korasick DA, Končitíková R, Kopečná M, Hájková E, Vigouroux A, Moréra S, Becker DF, Šebela M, Tanner JJ, Kopečný D
RgGuinier 4.1 nm
Dmax 14.4 nm
VolumePorod 351 nm3

SASDEA6 – Staphylococcus aureus N-acetylglucosamine-6-phosphate deacetylase dimer

UniProt ID: A0A0H2XGG9 (None-None) N-acetylglucosamine-6-phosphate deacetylase
N-acetylglucosamine-6-phosphate deacetylase experimental SAS data
PYMOL model
Sample: N-acetylglucosamine-6-phosphate deacetylase dimer, 86 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris-HCl 150 mM NaCl, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Apr 26
Functional and solution structure studies of amino sugar deacetylase and deaminase enzymes from Staphylococcus aureus. FEBS Lett (2018)
Davies JS, Coombes D, Horne CR, Pearce FG, Friemann R, North RA, Dobson RCJ
RgGuinier 3.2 nm
Dmax 10.1 nm
VolumePorod 107 nm3

SASDEB6 – Staphylococcus aureus glucosamine-6-phosphate deaminase

UniProt ID: A8YZR7 (None-None) Glucosamine-6-phosphate deaminase
Glucosamine-6-phosphate deaminase experimental SAS data
PYMOL model
Sample: Glucosamine-6-phosphate deaminase dimer, 57 kDa Staphylococcus aureus protein
Buffer: 20 mM Tris-HCl 150 mM NaCl, pH: 8
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2016 Apr 26
Functional and solution structure studies of amino sugar deacetylase and deaminase enzymes from Staphylococcus aureus. FEBS Lett (2018)
Davies JS, Coombes D, Horne CR, Pearce FG, Friemann R, North RA, Dobson RCJ
RgGuinier 2.7 nm
Dmax 8.9 nm
VolumePorod 84 nm3

SASDED6 – Proliferating cell nuclear antigen - UbPCNA - Split Fusion Trimer

UniProt ID: P15873 (None-None) Proliferating cell nuclear antigen
Proliferating cell nuclear antigen experimental SAS data
UIOWA_BD model
Sample: Proliferating cell nuclear antigen trimer, 117 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Aug 6
Conformational Flexibility of Ubiquitin-Modified and SUMO-Modified PCNA Shown by Full-Ensemble Hybrid Methods. J Mol Biol 430(24):5294-5303 (2018)
Powers KT, Lavering ED, Washington MT
RgGuinier 4.1 nm
Dmax 14.0 nm
VolumePorod 238 nm3

SASDEE6 – Proliferating cell nuclear antigen - SUMOPCNA - Split Fusion Trimer

UniProt ID: P15873 (None-None) Proliferating cell nuclear antigen
Proliferating cell nuclear antigen experimental SAS data
UIOWA_BD model
Sample: Proliferating cell nuclear antigen trimer, 125 kDa Saccharomyces cerevisiae protein
Buffer: 20 mM Tris, 150 mM NaCl, 5% glycerol, 1 mM TCEP, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2018 Aug 6
Conformational Flexibility of Ubiquitin-Modified and SUMO-Modified PCNA Shown by Full-Ensemble Hybrid Methods. J Mol Biol 430(24):5294-5303 (2018)
Powers KT, Lavering ED, Washington MT
RgGuinier 4.7 nm
Dmax 16.9 nm
VolumePorod 310 nm3

SASDEF6 – Epstein-Barr nuclear antigen 2 (EBNA2 Type1, amino acids 381-455)

UniProt ID: P12978 (381-455) Epstein-Barr nuclear antigen 2
Epstein-Barr nuclear antigen 2 experimental SAS data
DAMMIN model
Sample: Epstein-Barr nuclear antigen 2 dimer, 16 kDa Human gammaherpesvirus 4 protein
Buffer: 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 23
Increased association between Epstein-Barr virus EBNA2 from type 2 strains and the transcriptional repressor BS69 restricts B cell growth (2018)
Ponnusamy R, Khatri R, Correia P, Mancini E, Farrell P, West M
RgGuinier 2.7 nm
Dmax 9.3 nm
VolumePorod 19 nm3

SASDEG6 – Epstein-Barr nuclear antigen 2 (EBNA2 Type2, amino acids 348-422)

UniProt ID: Q69022 (348-422) Epstein-Barr nuclear antigen 2
Epstein-Barr nuclear antigen 2 experimental SAS data
DAMMIN model
Sample: Epstein-Barr nuclear antigen 2 dimer, 16 kDa Human gammaherpesvirus 4 protein
Buffer: 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 23
Increased association between Epstein-Barr virus EBNA2 from type 2 strains and the transcriptional repressor BS69 restricts B cell growth (2018)
Ponnusamy R, Khatri R, Correia P, Mancini E, Farrell P, West M
RgGuinier 2.8 nm
Dmax 10.4 nm
VolumePorod 20 nm3

SASDEH6 – BS69 (CC-MYND: Zinc finger MYND domain-containing protein 11, amino acids 480-602)

UniProt ID: Q15326-1 (480-602) Zinc finger MYND domain-containing protein 11
Zinc finger MYND domain-containing protein 11 experimental SAS data
DAMMIN model
Sample: Zinc finger MYND domain-containing protein 11 tetramer, 60 kDa Homo sapiens protein
Buffer: 20mM Tris-HCl, 100mM NaCl, 2% Sucrose and 1mM TCEP, pH: 7.5
Experiment: SAXS data collected at B21, Diamond Light Source on 2017 Sep 23
Increased association between Epstein-Barr virus EBNA2 from type 2 strains and the transcriptional repressor BS69 restricts B cell growth (2018)
Ponnusamy R, Khatri R, Correia P, Mancini E, Farrell P, West M
RgGuinier 5.3 nm
Dmax 16.9 nm
VolumePorod 83 nm3