SASBDB entries for UniProt ID:

SASDE85 – Bovine serum albumin monomer from in-house SEC-SAXS

UniProt ID: P02769 (25-607) Bovine serum albumin
Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 8.2 nm
VolumePorod 85 nm3

SASDE95 – Bovine serum albumin monomer from in-house SEC-SAXS

UniProt ID: P02769 (25-607) Bovine serum albumin
Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.9 nm
Dmax 8.6 nm
VolumePorod 74 nm3

SASDEA5 – Bovine serum albumin monomer from in-house SEC-SAXS

UniProt ID: P02769 (25-607) Bovine serum albumin
Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 25
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.6 nm
VolumePorod 78 nm3

SASDEB5 – Bovine serum albumin from in-house SAXS

UniProt ID: P02769 (25-607) Bovine serum albumin
Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 May 4
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.4 nm
VolumePorod 99 nm3

SASDEC5 – Bovine serum albumin dimer from in-house SEC-SAXS

UniProt ID: P02769 (25-607) Bovine serum albumin
Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin dimer, 133 kDa Bos taurus protein
Buffer: PBS, pH: 7.4
Experiment: SAXS data collected at Xenocs BioXolver L with MetalJet, University of Copenhagen, Department of Drug Design and Pharmacology on 2018 Jan 23
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 4.1 nm
Dmax 13.8 nm
VolumePorod 200 nm3

SASDED5 – Bovine serum albumin monomer from synchrotron SEC-SAXS

UniProt ID: P02769 (25-607) Bovine serum albumin
Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: PBS + 1 mM DTT, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2018 Jun 14
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.7 nm
Dmax 8.7 nm
VolumePorod 84 nm3

SASDEE5 – Bovine serum albumin from synchrotron SAXS

UniProt ID: P02769 (25-607) Bovine serum albumin
Bovine serum albumin experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer: 50 mM HEPES, pH: 7.5
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Nov 26
Size-exclusion chromatography small-angle X-ray scattering of water soluble proteins on a laboratory instrument. J Appl Crystallogr 51(Pt 6):1623-1632 (2018)
Bucciarelli S, Midtgaard SR, Nors Pedersen M, Skou S, Arleth L, Vestergaard B
RgGuinier 2.8 nm
Dmax 9.5 nm
VolumePorod 85 nm3

SASDEF5 – Phosphoketolase (L. lactis) with 1 mM thiaminpyrophosphate and 2% v/v glycerol at pH 7.0

UniProt ID: Q9CFH4 (None-None) Probable phosphoketolase
Probable phosphoketolase experimental SAS data
PDB (PROTEIN DATA BANK) model
Sample: Probable phosphoketolase dimer, 191 kDa Lactococcus lactis subsp. … protein
Buffer: 20mM potassium phosphate 150mM NaCl 0.007 %(w/v) β-octyl glucoside 1mM DTT 1mM MgCl 1mM thiaminpyrophosphate 2 %(v/v) glycerol, pH: 7
Experiment: SAXS data collected at EMBL P12, PETRA III on 2017 Sep 7
Crystal structure of a xylulose 5-phosphate phosphoketolase. insights into the substrate specificity for xylulose 5-phosphate. J Struct Biol (2019)
Scheidig AJ, Horvath D, Szedlacsek SE
RgGuinier 3.4 nm
Dmax 10.2 nm
VolumePorod 237 nm3

SASDEG5 – apo human adenylosuccinate lyase (ADSL)

UniProt ID: P30566 (1-484) Adenylosuccinate Lyase
Adenylosuccinate Lyase experimental SAS data
Adenylosuccinate Lyase Kratky plot
Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2015 Nov 13
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.7 nm
VolumePorod 300 nm3

SASDEH5 – AMP/fumarate-bound human adenylosuccinate lyase (ADSL)

UniProt ID: P30566 (1-484) Adenylosuccinate Lyase
Adenylosuccinate Lyase experimental SAS data
Adenylosuccinate Lyase Kratky plot
Sample: Adenylosuccinate Lyase tetramer, 221 kDa Homo sapiens protein
Buffer: 10 mM HEPES, 100 mM NaCl, 1 mM DTT, 1mM AMP, 1mM Fumarate, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2016 Jul 24
Molecular comparison of Neanderthal and Modern Human adenylosuccinate lyase. Sci Rep 8(1):18008 (2018)
Van Laer B, Kapp U, Soler-Lopez M, Moczulska K, Pääbo S, Leonard G, Mueller-Dieckmann C
RgGuinier 3.6 nm
VolumePorod 256 nm3