Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID: I6X8D2

SASDNK9 – Monomer of the full-length apo-polyketide synthase Pks13 of Mycobacterium tuberculosis

UniProt ID: I6X8D2 (1-1733) Polyketide synthase Pks13

Polyketide synthase Pks13 experimental SAS data

Sample:	Polyketide synthase Pks13 monomer, 188 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Mar 1

Solution structure of the type I polyketide synthase Pks13 from Mycobacterium tuberculosis. BMC Biol 20(1):147 (2022)
Bon C, Cabantous S, Julien S, Guillet V, Chalut C, Rima J, Brison Y, Malaga W, Sanchez-Dafun A, Gavalda S, Quémard A, Marcoux J, Waldo GS, Guilhot C, Mourey L

R_g^Guinier	7.3	nm
D_max	24.8	nm
Volume^Porod	480	nm³

SASDNL9 – Full length apo-polyketide synthase Pks13(S1533A) with Ser801 loaded by a C16-carbon chain

UniProt ID: I6X8D2 (1-1733) Polyketide synthase Pks13

Sample:	Polyketide synthase Pks13 dimer, 376 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2011 Nov 23

R_g^Guinier	10.3	nm
D_max	36.0	nm
Volume^Porod	2300	nm³

SASDNM9 – Thioesterase domain of polyketide synthase Pks13 at neutral pH

UniProt ID: I6X8D2 (1437-1725) Polyketide synthase Pks13

Sample:	Polyketide synthase Pks13 monomer, 33 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

R_g^Guinier	1.9	nm
D_max	5.5	nm
Volume^Porod	56	nm³

SASDNN9 – di-domain sub fragment ACP2TE of polyketide synthase Pks13 at neutral pH

UniProt ID: I6X8D2 (1154-1720) Polyketide synthase Pks13

Sample:	Polyketide synthase Pks13 monomer, 62 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl 50 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

R_g^Guinier	3.2	nm
D_max	12.0	nm
Volume^Porod	80	nm³

SASDNP9 – AT52: acyltransferase domain and insertion domain of polyketide synthase Pks13 at neutral pH

UniProt ID: I6X8D2 (576-1063) Polyketide synthase Pks13

Sample:	Polyketide synthase Pks13 monomer, 53 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

R_g^Guinier	3.5	nm
D_max	12.0	nm
Volume^Porod	115	nm³

SASDNQ9 – Acyltransferase domain of polyketide synthase Pks13 at neutral pH

UniProt ID: I6X8D2 (591-1046) Polyketide synthase Pks13

Sample:	Polyketide synthase Pks13 monomer, 51 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 300 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2011 Jun 3

R_g^Guinier	2.8	nm
D_max	8.0	nm
Volume^Porod	85	nm³

SASDNR9 – Subfragment ACP1KSAT tridomain of polyketide synthase Pks13 at neutral pH

UniProt ID: I6X8D2 (1-1063) Polyketide synthase Pks13

Sample:	Polyketide synthase Pks13 monomer, 116 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl 50 mM NaCl, pH: 8.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2006 Nov 1

R_g^Guinier	3.8	nm
D_max	12.9	nm
Volume^Porod	186	nm³

SASDNS9 – KS domain of polyketide synthase Pks13 at neutral pH

UniProt ID: I6X8D2 (119-575) Polyketide synthase Pks13

Sample:	Polyketide synthase Pks13 monomer, 51 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2012 Dec 7

R_g^Guinier	2.9	nm
D_max	10.0	nm
Volume^Porod	82	nm³

SASDNT9 – Subfragment KSAT of Polyketide synthase Pks13 at neutral pH

UniProt ID: I6X8D2 (119-1070) Polyketide synthase Pks13

Sample:	Polyketide synthase Pks13 monomer, 104 kDa Mycobacterium tuberculosis (strain … protein
Buffer:	50 mM Tris-HCl, 50 mM NaCl, pH: 8
Experiment:	SAXS data collected at SWING, SOLEIL on 2008 Sep 20

R_g^Guinier	3.8	nm
D_max	13.0	nm
Volume^Porod	155	nm³