Small Angle Scattering Biological Data Bank SASBDB

SASBDB entries for UniProt ID: P02769

SASDP87 – Bovine serum albumin at neutral pH, 50 °C, and 5 MPa (1 atm) of pressure

UniProt ID: P02769 (1-607) Albumin

Sample:	Albumin monomer, 69 kDa Bos taurus protein
Buffer:	25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7

Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R

R_g^Guinier	3.0	nm
D_max	9.5	nm
Volume^Porod	108	nm³

SASDP97 – Bovine serum albumin at neutral pH, 50 °C, and 100 MPa (1000 atm) of pressure

UniProt ID: P02769 (1-607) Albumin

Sample:	Albumin monomer, 69 kDa Bos taurus protein
Buffer:	25 mM HEPES, 150 mM NaCl, and 1% v/v glycerol, pH: 7
Experiment:	SAXS data collected at ID7A1 BioSAXS / HP-Bio Beamline, Cornell High Energy Synchrotron Source (CHESS) on 2020 Mar 7

Inline small‐angle X‐ray scattering‐coupled chromatography under extreme hydrostatic pressure Protein Science 31(12) (2022)
Miller R, Cummings C, Huang Q, Ando N, Gillilan R

R_g^Guinier	2.8	nm
D_max	9.4	nm
Volume^Porod	104	nm³

SASDQX8 – Bovine serum albumin (oligomeric mixtures) - reservoir sample storage with liquid jet delivery measured using XFEL

UniProt ID: P02769 (25-607) Albumin

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 14

Form factor determination of biological molecules with X-ray free electron laser small-angle scattering (XFEL-SAS). Commun Biol 6(1):1057 (2023)
Blanchet CE, Round A, Mertens HDT, Ayyer K, Graewert M, Awel S, Franke D, Dörner K, Bajt S, Bean R, Custódio TF, de Wijn R, Juncheng E, Henkel A, Gruzinov A, Jeffries CM, Kim Y, Kirkwood H, Kloos M, Knoška J, Koliyadu J, Letrun R, Löw C, Makroczyova J, Mall A, Meijers R, Pena Murillo GE, Oberthür D, Round E, Seuring C, Sikorski M, Vagovic P, Valerio J, Wollweber T, Zhuang Y, Schulz J, Haas H, Chapman HN, Mancuso AP, Svergun D

R_g^Guinier

3.1

SASDQY8 – Bovine serum albumin (oligomeric mixtures) - direct liquid jet autosampler delivery measured using XFEL

UniProt ID: P02769 (25-607) Albumin

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	phosphate buffered saline, pH: 7.4
Experiment:	SANS data collected at SPB/SFX, European XFEL on 2021 May 15

R_g^Guinier

2.7

SASDRL8 – Bovine serum albumin

UniProt ID: P02769 (25-607) Albumin

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment:	SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2022 Jun 7

NSRRC TPS13A standard protein archive
Orion Shih

R_g^Guinier	2.9	nm
D_max	8.9	nm
Volume^Porod	84	nm³

SASDRQ8 – Bovine serum albumin monomer separated using asymmetric flow field flow fractionation (AFFFF)

UniProt ID: P02769 (25-607) Albumin

Sample:	Albumin monomer, 66 kDa Bos taurus protein
Buffer:	10 mM HEPES, 5 mM NaCl, 0.1 mM EDTA, pH: 7.4
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2022 Jun 8

Quantitative size-resolved characterization of mRNA nanoparticles by in-line coupling of asymmetrical-flow field-flow fractionation with small angle X-ray scattering. Sci Rep 13(1):15764 (2023)
Graewert MA, Wilhelmy C, Bacic T, Schumacher J, Blanchet C, Meier F, Drexel R, Welz R, Kolb B, Bartels K, Nawroth T, Klein T, Svergun D, Langguth P, Haas H

R_g^Guinier	2.9	nm
D_max	9.0	nm
Volume^Porod	104	nm³

SASDWB3 – Bovine serum albumin, monomer from SEC-SANS

UniProt ID: P02769 (None-None) Albumin (natural variant A214T)

Albumin (natural variant A214T) experimental SAS data

Sample:	Albumin (natural variant A214T) monomer, 66 kDa Bos taurus protein
Buffer:	100 mM Tris-HCl, 100 mM NaCl in 100% v/v D₂O, pH: 7.5
Experiment:	SANS data collected at SANS-U, JRR-3 (Japan Atomic Energy Agency) on 2024 Apr 22

Size-exclusion chromatography–small-angle neutron scattering system optimized for an instrument with medium neutron flux Journal of Applied Crystallography 58(2) (2025)
Morishima K, Inoue R, Nakagawa T, Shimizu M, Sakamoto R, Oda T, Mayumi K, Sugiyama M

R_g^Guinier	2.6	nm
D_max	8.7	nm

SASDFQ8 – Bovine serum albumin, purified monomer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

UniProt ID: P02769 (25-607) Bovine serum albumin

Bovine serum albumin experimental SAS data

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

Adding Size Exclusion Chromatography (SEC) and Light Scattering (LS) Devices to Obtain High-Quality Small Angle X-Ray Scattering (SAXS) Data Crystals 10(11):975 (2020)
Graewert M, Da Vela S, Gräwert T, Molodenskiy D, Blanchet C, Svergun D, Jeffries C

R_g^Guinier	2.8	nm
D_max	8.0	nm
Volume^Porod	98	nm³

SASDFR8 – Bovine serum albumin, purified dimer - SEC-SAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

UniProt ID: P02769 (25-607) Bovine serum albumin

Sample:	Bovine serum albumin dimer, 133 kDa Bos taurus protein
Buffer:	50 mM HEPES, 150 mM NaCl, 2% v/v glycerol, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Apr 5

R_g^Guinier	4.0	nm
D_max	13.2	nm
Volume^Porod	211	nm³

SASDF99 – Bovine serum albumin monomer - SEC-SAXS/WAXS coupled to multiangle laser and quasi-elastic light scattering (MALLS and QELS)

UniProt ID: P02769 (25-607) Bovine serum albumin

Sample:	Bovine serum albumin monomer, 66 kDa Bos taurus protein
Buffer:	50 mM HEPES, 3% v/v glycerol,, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Apr 23

R_g^Guinier	2.8	nm
D_max	8.3	nm
Volume^Porod	95	nm³

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