SASBDB entries for UniProt ID: P0ACD4

SASDAW6 – Iron-sulfur cluster assembly scaffold IscU monomer

UniProt ID: P0ACD4 (None-None) Iron-sulfur cluster assembly scaffold protein IscU

Iron-sulfur cluster assembly scaffold protein IscU experimental SAS data
PDB model
Sample: Iron-sulfur cluster assembly scaffold protein IscU monomer, 14 kDa Escherichia coli protein
Buffer: 20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2009 Nov 5
Structural bases for the interaction of frataxin with the central components of iron-sulphur cluster assembly. Nat Commun 1:95 (2010)
Prischi F, Konarev PV, Iannuzzi C, Pastore C, Adinolfi S, Martin SR, Svergun DI, Pastore A
RgGuinier 1.9 nm
Dmax 6.4 nm

SASDAY6 – IcsS-dimer and IscU-dimer complex

UniProt ID: P0A6B7 (None-None) Cysteine desulfurase IscS

UniProt ID: P0ACD4 (None-None) Iron-sulfur cluster assembly scaffold protein IscU

Cysteine desulfurase IscSIron-sulfur cluster assembly scaffold protein IscU experimental SAS data
DAMMIF model
Sample: Cysteine desulfurase IscS dimer, 87 kDa Escherichia coli protein
Iron-sulfur cluster assembly scaffold protein IscU dimer, 29 kDa Escherichia coli protein
Buffer: 20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2009 Nov 5
Structural bases for the interaction of frataxin with the central components of iron-sulphur cluster assembly. Nat Commun 1:95 (2010)
Prischi F, Konarev PV, Iannuzzi C, Pastore C, Adinolfi S, Martin SR, Svergun DI, Pastore A
RgGuinier 3.6 nm
Dmax 12.1 nm

SASDA27 – IcsS, IscU and CyaY dimeric complex

UniProt ID: P0A6B7 (None-None) Cysteine desulfurase IscS

UniProt ID: P0ACD4 (None-None) Iron-sulfur cluster assembly scaffold protein IscU

UniProt ID: P27838 (None-None) Protein CyaY

Cysteine desulfurase IscSIron-sulfur cluster assembly scaffold protein IscUProtein CyaY experimental SAS data
SASREF model
Sample: Cysteine desulfurase IscS dimer, 87 kDa Escherichia coli protein
Iron-sulfur cluster assembly scaffold protein IscU dimer, 29 kDa Escherichia coli protein
Protein CyaY dimer, 24 kDa Escherichia coli protein
Buffer: 20 mM Tris-HCl 150 mM NaCl 10 mM β-mercaptoethanol, pH: 8
Experiment: SAXS data collected at EMBL X33, DORIS III on 2009 Nov 5
Structural bases for the interaction of frataxin with the central components of iron-sulphur cluster assembly. Nat Commun 1:95 (2010)
Prischi F, Konarev PV, Iannuzzi C, Pastore C, Adinolfi S, Martin SR, Svergun DI, Pastore A
RgGuinier 3.5 nm
Dmax 11.9 nm