SASBDB entries for UniProt ID: P11532

SASDDK9 – Conformation of R1-3 human dystrophin fragment in interaction with zwitterionic phospholipid bicelles (SANS)

UniProt ID: P11532 (338-668) R1-3 human dystrophin fragment
R1-3 human dystrophin fragment experimental SAS data
DAMMIF model
Sample: R1-3 human dystrophin fragment monomer, 39 kDa Homo sapiens protein
Buffer: 20 mM Tris-d11, 150 mM NaCl, 0.1 mM EDTA-d16, in 100% D2O, pD 7.5, pH: 7.1
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Nov 7
Human Dystrophin Structural Changes upon Binding to Anionic Membrane Lipids. Biophys J 115(7):1231-1239 (2018)
Dos Santos Morais R, Delalande O, Pérez J, Mias-Lucquin D, Lagarrigue M, Martel A, Molza AE, Chéron A, Raguénès-Nicol C, Chenuel T, Bondon A, Appavou MS, Le Rumeur E, Combet S, Hubert JF
RgGuinier 4.1 nm
Dmax 17.8 nm
VolumePorod 50 nm3

SASDDL9 – Conformation of R1-3 human dystrophin fragment in interaction with anionic phospholipid bicelles (SANS)

UniProt ID: P11532 (338-668) R1-3 human dystrophin fragment
R1-3 human dystrophin fragment experimental SAS data
YASARA model
Sample: R1-3 human dystrophin fragment monomer, 39 kDa Homo sapiens protein
Buffer: 20 mM Tris-d11, 150 mM NaCl, 0.1 mM EDTA-d16, in 100% D2O, pD 7.5, pH: 7.1
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Nov 7
Human Dystrophin Structural Changes upon Binding to Anionic Membrane Lipids. Biophys J 115(7):1231-1239 (2018)
Dos Santos Morais R, Delalande O, Pérez J, Mias-Lucquin D, Lagarrigue M, Martel A, Molza AE, Chéron A, Raguénès-Nicol C, Chenuel T, Bondon A, Appavou MS, Le Rumeur E, Combet S, Hubert JF
RgGuinier 6.2 nm
Dmax 24.8 nm
VolumePorod 100 nm3

SASDFY4 – R4-15 human dystrophin fragment

UniProt ID: P11532 (718-1973) Human dystrophin central domain R4-15 fragment
Human dystrophin central domain R4-15 fragment experimental SAS data
Human dystrophin central domain R4-15 fragment Kratky plot
Sample: Human dystrophin central domain R4-15 fragment monomer, 150 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2016 May 26
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 9.7 nm
Dmax 47.5 nm

SASDFZ4 – R16-24 human dystrophin fragment

UniProt ID: P11532 (1990-3040) Human dystrophin central domain R16-24 fragment
Human dystrophin central domain R16-24 fragment experimental SAS data
Human dystrophin central domain R16-24 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 fragment monomer, 127 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2016 Dec 17
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 9.3 nm
Dmax 34.0 nm

SASDF25 – R16-24 del45-55 human dystrophin fragment

UniProt ID: P11532 (1990-3040) Human dystrophin central domain R16-24 del45-55 fragment
Human dystrophin central domain R16-24 del45-55 fragment experimental SAS data
Human dystrophin central domain R16-24 del45-55 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 del45-55 fragment monomer, 58 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Sep 28
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 4.7 nm
Dmax 18.3 nm

SASDF35 – R16-24 del45-51 human dystrophin fragment

UniProt ID: P11532 (1990-3040) Human dystrophin central domain R16-24 del45-51 fragment
Human dystrophin central domain R16-24 del45-51 fragment experimental SAS data
Human dystrophin central domain R16-24 del45-51 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 del45-51 fragment monomer, 85 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycérol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Jul 13
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 7.0 nm
Dmax 26.2 nm

SASDGM2 – R16-24 del44-54 human dystrophin fragment

UniProt ID: P11532 (1990-3040) Human dystrophin central domain R16-24 del44-54 fragment
Human dystrophin central domain R16-24 del44-54 fragment experimental SAS data
Human dystrophin central domain R16-24 del44-54 fragment Kratky plot
Sample: Human dystrophin central domain R16-24 del44-54 fragment monomer, 57 kDa Homo sapiens protein
Buffer: 20 mM Na-phosphate, 300 mM NaCl, 1 mM EDTA, 2% glycerol,, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2019 Jul 8
Dystrophin SAXS data
Raphael Dos Santos Morais
RgGuinier 6.4 nm
Dmax 25.0 nm
VolumePorod 159 nm3

SASDFW4 – Conformation of R8-15 human dystrophin fragment

UniProt ID: P11532 (1156-1973) Human dystrophin central domain R8-15 fragment
Human dystrophin central domain R8-15 fragment experimental SAS data
CUSTOM IN-HOUSE model
Sample: Human dystrophin central domain R8-15 fragment monomer, 100 kDa protein
Buffer: NaP 10 mM, NaCl 500 mM, EDTA 1 mM, Glycerol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2015 Sep 23
How the central domain of dystrophin acts to bridge F-actin to sarcolemmal lipids. J Struct Biol :107411 (2019)
Mias-Lucquin D, Dos Santos Morais R, Chéron A, Lagarrigue M, Winder SJ, Chenuel T, Pérez J, Appavou MS, Martel A, Alviset G, Le Rumeur E, Combet S, Hubert JF, Delalande O
RgGuinier 10.1 nm
Dmax 36.0 nm

SASDFX4 – Conformation of R11-19 human dystrophin fragment

UniProt ID: P11532 (1461-2420) Human dystrophin central domain R11-19 fragment
Human dystrophin central domain R11-19 fragment experimental SAS data
CUSTOM IN-HOUSE model
Sample: Human dystrophin central domain R11-19 fragment monomer, 117 kDa protein
Buffer: NaP 20 mM, NaCl 300 mM, EDTA 1 mM, Glycerol 2%, pH: 7.5
Experiment: SAXS data collected at SWING, SOLEIL on 2017 May 11
How the central domain of dystrophin acts to bridge F-actin to sarcolemmal lipids. J Struct Biol :107411 (2019)
Mias-Lucquin D, Dos Santos Morais R, Chéron A, Lagarrigue M, Winder SJ, Chenuel T, Pérez J, Appavou MS, Martel A, Alviset G, Le Rumeur E, Combet S, Hubert JF, Delalande O
RgGuinier 8.8 nm
Dmax 37.5 nm
VolumePorod 513 nm3

SASDFT4 – Conformation of the R11-15 human dystrophin fragment (SANS)

UniProt ID: P11532 (1461-1973) Dystrophin (R11-15 human dystrophin fragment)
Dystrophin (R11-15 human dystrophin fragment) experimental SAS data
CUSTOM IN-HOUSE model
Sample: Dystrophin (R11-15 human dystrophin fragment) monomer, 60 kDa Homo sapiens protein
Buffer: 20 mM Tris-d11, 150 mM NaCl, 0.1 mM EDTA-d16, in 100% v/v D2O, pH: 7.1
Experiment: SANS data collected at D22, Institut Laue-Langevin (ILL) on 2016 Nov 1
How the central domain of dystrophin acts to bridge F-actin to sarcolemmal lipids. J Struct Biol :107411 (2019)
Mias-Lucquin D, Dos Santos Morais R, Chéron A, Lagarrigue M, Winder SJ, Chenuel T, Pérez J, Appavou MS, Martel A, Alviset G, Le Rumeur E, Combet S, Hubert JF, Delalande O
RgGuinier 6.2 nm
Dmax 27.4 nm
VolumePorod 146 nm3