SASBDB entries for UniProt ID: P20809

SASDLM3 – Interleukin-11 signalling complex, truncated gp130

UniProt ID: P40189 (22-324) Interleukin-6 receptor subunit beta

UniProt ID: P20809 (32-199) Interleukin-11

UniProt ID: Q14626 (23-319) Interleukin-11 receptor subunit alpha
Interleukin-6 receptor subunit betaInterleukin-11Interleukin-11 receptor subunit alpha experimental SAS data
OTHER model
Sample: Interleukin-6 receptor subunit beta dimer, 69 kDa Homo sapiens protein
Interleukin-11 dimer, 36 kDa Homo sapiens protein
Interleukin-11 receptor subunit alpha dimer, 64 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 8
Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant Nature Communications 14(1) (2023)
Metcalfe R, Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 5.3 nm
Dmax 17.6 nm
VolumePorod 405 nm3

SASDLN3 – Interleukin 11 signalling complex, full-length extracellular gp130

UniProt ID: P20809 (32-199) Interleukin-11

UniProt ID: Q14626 (23-319) Interleukin-11 receptor subunit alpha

UniProt ID: P40189 (23-612) Interleukin-6 receptor subunit beta
Interleukin-11Interleukin-11 receptor subunit alphaInterleukin-6 receptor subunit beta experimental SAS data
OTHER model
Sample: Interleukin-11 dimer, 36 kDa Homo sapiens protein
Interleukin-11 receptor subunit alpha dimer, 64 kDa Homo sapiens protein
Interleukin-6 receptor subunit beta dimer, 134 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 28
Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant Nature Communications 14(1) (2023)
Metcalfe R, Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 6.2 nm
Dmax 20.7 nm
VolumePorod 710 nm3

SASDLS3 – Interleukin 11 Mutein, complex with gp130 (domains 1-3) and IL-11Rα

UniProt ID: Q14626 (23-319) Interleukin-11 receptor subunit alpha

UniProt ID: None (None-None) Interleukin 11 Mutein

UniProt ID: P40189 (22-324) Interleukin-6 receptor subunit beta
Interleukin-11 receptor subunit alphaInterleukin 11 MuteinInterleukin-6 receptor subunit beta experimental SAS data
DAMMIN model
Sample: Interleukin-11 receptor subunit alpha monomer, 32 kDa Homo sapiens protein
Interleukin 11 Mutein monomer, 18 kDa Homo sapiens protein
Interleukin-6 receptor subunit beta monomer, 35 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 8
Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant Nature Communications 14(1) (2023)
Metcalfe R, Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 4.4 nm
Dmax 15.6 nm
VolumePorod 160 nm3

SASDLR3 – Interleukin 11 Mutein

UniProt ID: None (None-None) Interleukin 11 Mutein
Interleukin 11 Mutein experimental SAS data
OTHER model
Sample: Interleukin 11 Mutein monomer, 18 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Jun 8
Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant Nature Communications 14(1) (2023)
Metcalfe R, Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 1.8 nm
Dmax 5.4 nm
VolumePorod 25 nm3

SASDLQ3 – Interleukin 11, W168A mutant

UniProt ID: P20809 (32-199) Interleukin-11 (W168A)
Interleukin-11 (W168A) experimental SAS data
OTHER model
Sample: Interleukin-11 (W168A) monomer, 18 kDa Homo sapiens protein
Buffer: 20 mM Tris, 150 mM NaCl, 0.2% sodium azide, pH: 8.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2019 Nov 28
Structures of the interleukin 11 signalling complex reveal gp130 dynamics and the inhibitory mechanism of a cytokine variant Nature Communications 14(1) (2023)
Metcalfe R, Hanssen E, Fung K, Aizel K, Kosasih C, Zlatic C, Doughty L, Morton C, Leis A, Parker M, Gooley P, Putoczki T, Griffin M
RgGuinier 1.7 nm
Dmax 5.2 nm
VolumePorod 22 nm3