SASBDB entries for UniProt ID: P35555

SASDNR3 – Fibrillin-1 fragment PF3

UniProt ID: P35555 (1-722) Fibrillin-1 PF3
Fibrillin-1 PF3 experimental SAS data
DAMMIF model
Sample: Fibrillin-1 PF3 monomer, 78 kDa Homo sapiens protein
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2016 Dec 12
Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation. Matrix Biol (2022)
Lockhart-Cairns MP, Cain SA, Dajani R, Steer R, Thomson J, Alanazi YF, Kielty CM, Baldock C
RgGuinier 5.4 nm
Dmax 24.0 nm
VolumePorod 141 nm3

SASDNT3 – Fibrillin-1 fragment PF3 crosslinked to latent transforming growth factor-beta-binding protein 1 C-terminus by transglutaminase 2

UniProt ID: Q14766-2 (1329-1721) Latent-transforming growth factor beta-binding protein 1

UniProt ID: P35555 (1-722) Fibrillin-1 PF3
Latent-transforming growth factor beta-binding protein 1Fibrillin-1 PF3 experimental SAS data
DAMMIF model
Sample: Latent-transforming growth factor beta-binding protein 1 monomer, 44 kDa Homo sapiens protein
Fibrillin-1 PF3 monomer, 78 kDa Homo sapiens protein
Buffer: 10 mM Hepes, 150 mM NaCl, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2017 Jul 20
Latent TGFβ complexes are transglutaminase cross-linked to fibrillin to facilitate TGFβ activation. Matrix Biol (2022)
Lockhart-Cairns MP, Cain SA, Dajani R, Steer R, Thomson J, Alanazi YF, Kielty CM, Baldock C
RgGuinier 7.4 nm
Dmax 29.6 nm
VolumePorod 759 nm3

SASDNU3 – Fibrillin PF2

UniProt ID: P35555 (330-722) Fibrillin-1
Fibrillin-1 experimental SAS data
DAMMIF model
Sample: Fibrillin-1 monomer, 44 kDa Homo sapiens protein
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at ID02, ESRF on 2006 Feb 11
Transglutaminase-Mediated Cross-Linking of Tropoelastin to Fibrillin Stabilises the Elastin Precursor Prior to Elastic Fibre Assembly. J Mol Biol 432(21):5736-5751 (2020)
Lockhart-Cairns MP, Newandee H, Thomson J, Weiss AS, Baldock C, Tarakanova A
RgGuinier 4.3 nm
Dmax 15.0 nm
VolumePorod 84 nm3

SASDNW3 – Fibrillin-1 fragment PF2 crosslinked to tropoelastin by transglutaminase 2

UniProt ID: P35555 (330-722) Fibrillin-1

UniProt ID: P15502 (27-724) Elastin
Fibrillin-1Elastin experimental SAS data
DAMMIF model
Sample: Fibrillin-1 monomer, 44 kDa Homo sapiens protein
Elastin monomer, 60 kDa Homo sapiens protein
Buffer: Tris buffered saline, pH: 7.4
Experiment: SAXS data collected at B21, Diamond Light Source on 2018 Feb 21
Transglutaminase-Mediated Cross-Linking of Tropoelastin to Fibrillin Stabilises the Elastin Precursor Prior to Elastic Fibre Assembly. J Mol Biol 432(21):5736-5751 (2020)
Lockhart-Cairns MP, Newandee H, Thomson J, Weiss AS, Baldock C, Tarakanova A
RgGuinier 7.1 nm
Dmax 23.5 nm