Curated repository for small angle scattering data and models


SASBDB currently contains:


4780

experimental data sets

5958

models
568 experimental data sets on hold
799 models on hold

Small angle scattering (SAS) of X-ray and neutrons provides structural information on biological macromolecules in solution at a resolution of 1-2 nm.
SASBDB is a fully searchable curated repository of freely accessible and downloadable experimental data, which are deposited together with the relevant experimental conditions, sample details, derived models and their fits to the data.

Recent depositions:

SASDX54 – Precursor microRNA 20a (pre-miR-20a)

Precursor microRNA 20a experimental SAS data
AMBER model
Sample: Precursor microRNA 20a monomer, 23 kDa Homo sapiens RNA
Buffer: 50 mM potassium phosphate buffer, 1 mM MgCl2, 50 mM NaCl, 5 mM BME, pH: 7.5
Experiment: SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Oct 8
Structural features within precursor microRNA-20a regulate Dicer-TRBP processing Journal of Molecular Biology :169317 (2025)
Liu Y, Harkner C, Westwood M, Munsayac A, Keane S
RgGuinier 3.1 nm
Dmax 11.2 nm
VolumePorod 32 nm3

Yeast alcohol dehydrogenase 1 from AF4-SAXS measurements
Alcohol dehydrogenase 1 experimental SAS data
AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline. J Synchrotron Radiat 2025 Jul 1;
J Synchrotron Radiat (2025 Jul 1)
RTX719. Double-acylated truncated variant of B. pertussis adenylate cyclase toxin (CyaA)
Double-acylated variant of a genetic fusion comprising the C-terminal folding scaffold of RTX block V (residues 1562–1681 of CyaA) and the CyaA residues 719–1294 experimental SAS data
Acyl chains stabilize the acylated domain and determine the receptor-mediated interaction of the Bordetella adenylate cyclase toxin with cell membrane. J Biol Chem 2025 Jun 19;:110392
J Biol Chem :110392 (2025 Jun 19)
PDZ domain of the segment polarity protein dishevelled homolog DVL-3
Segment polarity protein dishevelled homolog DVL-3 experimental SAS data
A class III ligand oscillates between internal and terminal binding modes as it engages with the Dishevelled PDZ domain. Structure 2025 Jun 5;
Structure (2025 Jun 5)
Tetrameric L-threonine dehydratase biosynthetic IlvA (EcIlvA) from Eschericia coli
L-threonine dehydratase biosynthetic IlvA experimental SAS data
Isoleucine Binding and Regulation of Escherichia coli and Staphylococcus aureus Threonine Dehydratase (IlvA). Biochemistry 2025 Jun 10;
Biochemistry (2025 Jun 10)
Wild-type phosphoserine phosphatase RsbU dimer
Phosphoserine phosphatase RsbU experimental SAS data
A general mechanism for initiating the bacterial general stress response. Elife 2025 Jun 6;13
Elife 13 (2025 Jun 6)

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To cite SASBDB refer to: Kikhney AG, Borges CR, Molodenskiy DS, Jeffries CM, Svergun DI (2020) SASBDB: Towards an automatically curated and validated repository for biological scattering data. Protein Science 29(1); 66-75.