Small Angle Scattering Biological Data Bank SASBDB

SASDS33 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 5 mg/mL

C3H1-type domain-containing proteinsingle stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence experimental SAS data

C3H1-type domain-containing protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Kratky plot

Sample:	C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9

Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks

R_g^Guinier	1.6	nm
D_max	5.6	nm
Volume^Porod	13	nm³

SASDS43 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 3 mg/mL

single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequenceC3H1-type domain-containing protein experimental SAS data

single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence C3H1-type domain-containing protein Kratky plot

Sample:	single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9

R_g^Guinier	1.5	nm
D_max	5.7	nm
Volume^Porod	13	nm³

SASDS53 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 2 mg/mL

Sample:	C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9

R_g^Guinier	1.5	nm
D_max	5.6	nm
Volume^Porod	13	nm³

SASDS63 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 6 mg/mL

Sample:	C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9

R_g^Guinier	1.5	nm
D_max	5.1	nm
Volume^Porod	13	nm³

SASDS73 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 4.5 mg/mL

Sample:	C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9

R_g^Guinier	1.5	nm
D_max	4.9	nm
Volume^Porod	13	nm³

SASDS83 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 3 mg/mL

Sample:	C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer:	20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9

R_g^Guinier	1.5	nm
D_max	5.3	nm
Volume^Porod	13	nm³

SASDSY7 – Karrikin insensitive 2, KAI2 protein (monomer peak)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot

Sample:	Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	2.3	nm
D_max	9.5	nm
Volume^Porod	58	nm³

SASDSZ7 – Karrikin insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (monomer peak)

Sample:	Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	2.9	nm
D_max	13.9	nm
Volume^Porod	60	nm³

SASDS28 – Karrikin insensitive 2, KAI2 protein (aggregate peak)

Sample:	Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	4.1	nm
D_max	15.8	nm
Volume^Porod	283	nm³

SASDS38 – Karrikin insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (aggregate peak)

Sample:	Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer:	20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment:	SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29

SAXS studies of KAI2 catalytic mutants
Sabrina Davies

R_g^Guinier	4.9	nm
D_max	20.0	nm
Volume^Porod	345	nm³

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