Small Angle Scattering Biological Data Bank SASBDB

SASDLT9 – von Willebrand factor C6-CK G2705R

von Willebrand factor C6-CK G2705R experimental SAS data

von Willebrand factor C6-CK G2705R Kratky plot

Sample:	von Willebrand factor C6-CK G2705R dimer, 42 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Aug 18

Structure and dynamics of the von Willebrand Factor C6 domain
Emma-Ruoqi Xu

R_g^Guinier	3.5	nm
D_max	16.5	nm
Volume^Porod	95	nm³

SASDLU9 – von Willebrand factor C6-CK G2705K

von Willebrand factor C6-CK G2705K experimental SAS data

von Willebrand factor C6-CK G2705K Kratky plot

Sample:	von Willebrand factor C6-CK G2705K dimer, 41 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Aug 18

Structure and dynamics of the von Willebrand Factor C6 domain
Emma-Ruoqi Xu

R_g^Guinier	3.5	nm
D_max	16.0	nm
Volume^Porod	106	nm³

SASDLV9 – von Willebrand factor D4N-CK wildtype

von Willebrand factor D4N-CK wildtype experimental SAS data

von Willebrand factor D4N-CK wildtype Kratky plot

Sample:	von Willebrand factor D4N-CK wildtype dimer, 211 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 23

Structure and dynamics of the von Willebrand Factor C6 domain
Emma-Ruoqi Xu

R_g^Guinier	7.8	nm
D_max	34.8	nm
Volume^Porod	637	nm³

SASDLW9 – von Willebrand factor D4N-CK G2705R

von Willebrand factor D4N-CK G2705R experimental SAS data

von Willebrand factor D4N-CK G2705R Kratky plot

Sample:	von Willebrand factor D4N-CK G2705R dimer, 211 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 23

Structure and dynamics of the von Willebrand Factor C6 domain
Emma-Ruoqi Xu

R_g^Guinier	11.4	nm
D_max	68.5	nm
Volume^Porod	1800	nm³

SASDLX9 – von Willebrand factor D4N-CK G2705K

von Willebrand factor D4N-CK G2705K experimental SAS data

von Willebrand factor D4N-CK G2705K Kratky plot

Sample:	von Willebrand factor D4N-CK G2705K dimer, 211 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 150 mM NaCl, 1 mM CaCl2, 1 mM MgCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2019 Jun 23

Structure and dynamics of the von Willebrand Factor C6 domain
Emma-Ruoqi Xu

R_g^Guinier	9.8	nm
D_max	52.0	nm
Volume^Porod	917	nm³

SASDRF8 – Plasmodium falciparum circumsporozoite protein N-terminal domain at pH 6.6

Circumsporozoite protein experimental SAS data

Circumsporozoite protein Kratky plot

Sample:	Circumsporozoite protein monomer, 11 kDa Plasmodium falciparum (isolate … protein
Buffer:	50 mM MES, 500 mM NaCl, 1 mM TCEP, pH: 6.6
Experiment:	SAXS data collected at SWING, SOLEIL on 2022 Sep 8

Biophysical characterization of the Plasmodium falciparum circumsporozoite protein's N‐terminal domain Protein Science (2023)
Geens R, Stanisich J, Beyens O, D'Hondt S, Thiberge J, Ryckebosch A, Groot A, Magez S, Vertommen D, Amino R, Winter H, Volkov A, Tompa P, Sterckx Y

R_g^Guinier	2.3	nm
D_max	9.1	nm
Volume^Porod	24	nm³

SASDS96 – Human Ubiquilin-1 (UBQLN1)

Ubiquilin-1 experimental SAS data

Ubiquilin-1 Kratky plot

Sample:	Ubiquilin-1 dimer, 125 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2023 Mar 22

Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation. Biophys J (2023)
Dao TP, Rajendran A, Galagedera SKK, Haws W, Castañeda CA

R_g^Guinier	4.6	nm
D_max	19.3	nm
Volume^Porod	264	nm³

SASDSA6 – Human Ubiquilin-2 (UBQLN2)

Ubiquilin-2 experimental SAS data

Ubiquilin-2 Kratky plot

Sample:	Ubiquilin-2 dimer, 131 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Jun 8

Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation. Biophys J (2023)
Dao TP, Rajendran A, Galagedera SKK, Haws W, Castañeda CA

R_g^Guinier	5.2	nm
D_max	22.5	nm
Volume^Porod	330	nm³

SASDSB6 – Human Ubiquilin-4 (UBQLN4)

Ubiquilin-4 experimental SAS data

Ubiquilin-4 Kratky plot

Sample:	Ubiquilin-4 dimer, 128 kDa Homo sapiens protein
Buffer:	20 mM sodium phosphate, 0.5 mM EDTA, 0.02 % NaN3, pH: 6.8
Experiment:	SAXS data collected at BioCAT 18ID, Advanced Photon Source (APS), Argonne National Laboratory on 2022 Feb 26

Short disordered N-termini & proline-rich domain are major regulators of UBQLN1/2/4 phase separation. Biophys J (2023)
Dao TP, Rajendran A, Galagedera SKK, Haws W, Castañeda CA

R_g^Guinier	5.1	nm
D_max	21.7	nm
Volume^Porod	333	nm³

SASDHZ9 – Condensin complex subunit 1, Ycs4

Condensin complex subunit 1 experimental SAS data

MONSA model

Sample:	Condensin complex subunit 1 monomer, 137 kDa Chaetomium thermophilum protein
Buffer:	25 mM Tris, 300 mM NaCl, 1mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 20

Molecular flexibility of the condensin subunit Ycs4 is modulated by kleisin binding
Karen Manalastas-Cantos

R_g^Guinier	5.2	nm
D_max	17.7	nm
Volume^Porod	296	nm³

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