Small Angle Scattering Biological Data Bank SASBDB

SASDHA6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within High Five insect cells

Woronin body major protein experimental SAS data

Sample:	Woronin body major protein , 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

Rapid screening of in cellulo grown protein crystals via a small-angle X-ray scattering/X-ray powder diffraction synergistic approach Journal of Applied Crystallography 53(5) (2020)
Lahey-Rudolph J, Schönherr R, Jeffries C, Blanchet C, Boger J, Ferreira Ramos A, Riekehr W, Triandafillidis D, Valmas A, Margiolaki I, Svergun D, Redecke L

SASDHB6 – In cellulo Woronin body major protein (HEX-1) crystals recombinantly expressed within Sf9 insect cells

Sample:	Woronin body major protein , 19 kDa Neurospora crassa protein
Buffer:	TBS (20 mM Tris, 150 mM NaCl),, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDJY5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within High Five insect cells

Inosine-5'-monophosphate dehydrogenase experimental SAS data

Inosine-5'-monophosphate dehydrogenase Kratky plot

Sample:	Inosine-5'-monophosphate dehydrogenase , 59 kDa Trypanosoma brucei brucei protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDJZ5 – In cellulo inosine-5'-monophosphate dehydrogenase (IMPDH) protein crystals recombinantly expressed within Sf9 insect cells

Sample:	Inosine-5'-monophosphate dehydrogenase , 59 kDa Trypanosoma brucei brucei protein
Buffer:	20 mM Tris, 150 mM NaCl,, pH: 7
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2017 Nov 21

SASDHP5 – Rov C - Regulator Of Virulence interconnected with the Csr system (hexameric transcriptional activator of type VI secretion gene expression)

Regulator Of Virulence interconnected with the Csr system experimental SAS data

Sample:	Regulator Of Virulence interconnected with the Csr system hexamer, 175 kDa Yersinia pseudotuberculosis protein
Buffer:	50 mM TRIS pH= 8, 500 mM NaCl, 5 mM DTT, 5 % v/v glycerol, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2016 Jun 18

RovC - a novel type of hexameric transcriptional activator promoting type VI secretion gene expression PLOS Pathogens 16(9):e1008552 (2020)
Knittel V, Sadana P, Seekircher S, Stolle A, Körner B, Volk M, Jeffries C, Svergun D, Heroven A, Scrima A, Dersch P, Mecsas J

R_g^Guinier	4.2	nm
D_max	12.4	nm
Volume^Porod	280	nm³

SASDHC6 – C-terminal domain-like carotenoid protein (CCP2), apo-dimer

C-terminal domain-like carotenoid protein experimental SAS data

Sample:	C-terminal domain-like carotenoid protein dimer, 33 kDa Tolypothrix sp. PCC … protein
Buffer:	10 mM potassium phosphate, 100 mM NaCl, pH: 7
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 15

Structural analysis of a new carotenoid-binding protein: the C-terminal domain homolog of the OCP Scientific Reports 10(1) (2020)
Dominguez-Martin M, Hammel M, Gupta S, Lechno-Yossef S, Sutter M, Rosenberg D, Chen Y, Petzold C, Ralston C, Polívka T, Kerfeld C

R_g^Guinier	2.5	nm
D_max	9.1	nm
Volume^Porod	55	nm³

SASDHD6 – C-terminal domain-like carotenoid protein (CCP2), holo-dimer

Sample:	C-terminal domain-like carotenoid protein dimer, 36 kDa Tolypothrix sp. PCC … protein
Buffer:	10 mM potassium phosphate, 100 mM NaCl, pH: 7
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 15

R_g^Guinier	2.5	nm
D_max	7.9	nm
Volume^Porod	62	nm³

SASDHE6 – C-terminal domain-like carotenoid protein (CCP2), apo-tetramer

Sample:	C-terminal domain-like carotenoid protein tetramer, 66 kDa Tolypothrix sp. PCC … protein
Buffer:	10 mM potassium phosphate, 100 mM NaCl, pH: 7
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 15

R_g^Guinier	3.2	nm
D_max	10.0	nm
Volume^Porod	110	nm³

SASDHF6 – C-terminal domain-like carotenoid protein (CCP2), holo-tetramer

Sample:	C-terminal domain-like carotenoid protein tetramer, 73 kDa Tolypothrix sp. PCC … protein
Buffer:	10 mM potassium phosphate, 100 mM NaCl, pH: 7
Experiment:	SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2019 May 15

R_g^Guinier	3.3	nm
D_max	12.1	nm
Volume^Porod	157	nm³

SASDFS7 – Talin-1 head amino acids 1-405(Δ139-168)

Talin-1 (Δ139-168), human experimental SAS data

Talin-1 head amino acids 1-405(Δ139-168) Rg histogram

Sample:	Talin-1 (Δ139-168), human monomer, 48 kDa Homo sapiens protein
Buffer:	50 mM sodium phosphate, 150mM NaCl, pH: 7.2
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2015 Sep 24

The F1 loop of talin head domain acts as a gatekeeper in integrin activation and clustering
Sampo Kukkurainen

R_g^Guinier	3.3	nm
D_max	13.3	nm
Volume^Porod	77	nm³

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