|
|
|
Sample: |
C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
|
Buffer: |
20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
|
Experiment: |
SAXS
data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
|
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
|
RgGuinier |
1.6 |
nm |
Dmax |
5.6 |
nm |
VolumePorod |
13 |
nm3 |
|
|
|
|
|
Sample: |
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
|
Buffer: |
20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
|
Experiment: |
SAXS
data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
|
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
|
RgGuinier |
1.5 |
nm |
Dmax |
5.7 |
nm |
VolumePorod |
13 |
nm3 |
|
|
|
|
|
Sample: |
C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
|
Buffer: |
20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
|
Experiment: |
SAXS
data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
|
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
|
RgGuinier |
1.5 |
nm |
Dmax |
5.6 |
nm |
VolumePorod |
13 |
nm3 |
|
|
|
|
|
Sample: |
C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
|
Buffer: |
20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
|
Experiment: |
SAXS
data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
|
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
|
RgGuinier |
1.5 |
nm |
Dmax |
5.1 |
nm |
VolumePorod |
13 |
nm3 |
|
|
|
|
|
Sample: |
C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
|
Buffer: |
20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
|
Experiment: |
SAXS
data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
|
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
|
RgGuinier |
1.5 |
nm |
Dmax |
4.9 |
nm |
VolumePorod |
13 |
nm3 |
|
|
|
|
|
Sample: |
C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
|
Buffer: |
20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
|
Experiment: |
SAXS
data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
|
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
|
RgGuinier |
1.5 |
nm |
Dmax |
5.3 |
nm |
VolumePorod |
13 |
nm3 |
|
|
|
|
|
Sample: |
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
|
Buffer: |
20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
|
Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
|
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
|
RgGuinier |
2.3 |
nm |
Dmax |
9.5 |
nm |
VolumePorod |
58 |
nm3 |
|
|
|
|
|
Sample: |
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
|
Buffer: |
20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
|
Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
|
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
|
RgGuinier |
2.9 |
nm |
Dmax |
13.9 |
nm |
VolumePorod |
60 |
nm3 |
|
|
|
|
|
Sample: |
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
|
Buffer: |
20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
|
Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
|
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
|
RgGuinier |
4.1 |
nm |
Dmax |
15.8 |
nm |
VolumePorod |
283 |
nm3 |
|
|
|
|
|
Sample: |
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
|
Buffer: |
20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
|
Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
|
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
|
RgGuinier |
4.9 |
nm |
Dmax |
20.0 |
nm |
VolumePorod |
345 |
nm3 |
|
|