SASDS33 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 5 mg/mL

C3H1-type domain-containing proteinsingle stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence experimental SAS data
C3H1-type domain-containing protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Kratky plot
Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
RgGuinier 1.6 nm
Dmax 5.6 nm
VolumePorod 13 nm3

SASDS43 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 3 mg/mL

single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequenceC3H1-type domain-containing protein experimental SAS data
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence C3H1-type domain-containing protein Kratky plot
Sample: single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
RgGuinier 1.5 nm
Dmax 5.7 nm
VolumePorod 13 nm3

SASDS53 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 8 at 2 mg/mL

C3H1-type domain-containing proteinsingle stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence experimental SAS data
C3H1-type domain-containing protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Kratky plot
Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 8
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
RgGuinier 1.5 nm
Dmax 5.6 nm
VolumePorod 13 nm3

SASDS63 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 6 mg/mL

C3H1-type domain-containing proteinsingle stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence experimental SAS data
C3H1-type domain-containing protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Kratky plot
Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
RgGuinier 1.5 nm
Dmax 5.1 nm
VolumePorod 13 nm3

SASDS73 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 4.5 mg/mL

C3H1-type domain-containing proteinsingle stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence experimental SAS data
C3H1-type domain-containing protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Kratky plot
Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
RgGuinier 1.5 nm
Dmax 4.9 nm
VolumePorod 13 nm3

SASDS83 – spikemoss tandem zinc finger (TZF) domain in complex with 9-mer RNA, pH 6 at 3 mg/mL

C3H1-type domain-containing proteinsingle stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence experimental SAS data
C3H1-type domain-containing protein single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence Kratky plot
Sample: C3H1-type domain-containing protein monomer, 8 kDa Selaginella moellendorffii protein
single stranded 9-mer RNA: tristetraprolin (TTP) optimal target sequence monomer, 3 kDa synthetic construct RNA
Buffer: 20 mM sodium phosphate, 100 mM KCl, 25 µM ZnSO4, 2 mM β-mercaptoethanol, 5 % glycerol, pH: 6
Experiment: SAXS data collected at 12.3.1 (SIBYLS), Advanced Light Source (ALS) on 2016 Nov 9
Solution scattering of the tandem zinc finger (TZF) domain from the tristetraprolin (TTP) family member found in Selaginella moellendorffii (spikemoss) in the presence and absence of 9-mer RNA
Stephanie Hicks
RgGuinier 1.5 nm
Dmax 5.3 nm
VolumePorod 13 nm3

SASDSY7 – Karrikin insensitive 2, KAI2 protein (monomer peak)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 2.3 nm
Dmax 9.5 nm
VolumePorod 58 nm3

SASDSZ7 – Karrikin insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (monomer peak)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 2.9 nm
Dmax 13.9 nm
VolumePorod 60 nm3

SASDS28 – Karrikin insensitive 2, KAI2 protein (aggregate peak)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 4.1 nm
Dmax 15.8 nm
VolumePorod 283 nm3

SASDS38 – Karrikin insensitive 2, KAI2 protein with a synthetic strigolactone analogue ligand (aggregate peak)

Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) experimental SAS data
Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) Kratky plot
Sample: Probable esterase KAI2 (KARRIKIN INSENSITIVE 2) monomer, 30 kDa Arabidopsis thaliana protein
Buffer: 20 mM HEPES, 150 mM imidazole, 150 mM NaCl, 10% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at SAXS/WAXS, Australian Synchrotron on 2022 Mar 29
SAXS studies of KAI2 catalytic mutants
Sabrina Davies
RgGuinier 4.9 nm
Dmax 20.0 nm
VolumePorod 345 nm3

4719 hits found.