Browse by DISSEMINATION: Published

SASDRG8 – Astaxanthin-binding protein orange1 (AstaPo1) complexed with zeaxanthin

Astaxanthin binding fasciclin family protein experimental SAS data
PYMOL model
Sample: Astaxanthin binding fasciclin family protein monomer, 22 kDa Coelastrella astaxanthina protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2021 Sep 24
Structural basis for the ligand promiscuity of the neofunctionalized, carotenoid-binding fasciclin domain protein AstaP. Commun Biol 6(1):471 (2023)
Kornilov FD, Slonimskiy YB, Lunegova DA, Egorkin NA, Savitskaya AG, Kleymenov SY, Maksimov EG, Goncharuk SA, Mineev KS, Sluchanko NN
RgGuinier 2.1 nm
Dmax 8.0 nm
VolumePorod 43 nm3

SASDRH8 – Astaxanthin-binding protein orange1 (AstaPo1) complexed with canthaxanthin

Astaxanthin binding fasciclin family protein experimental SAS data
PYMOL model
Sample: Astaxanthin binding fasciclin family protein monomer, 22 kDa Coelastrella astaxanthina protein
Buffer: 20 mM Tris-HCl, 150 mM NaCl, pH: 7.6
Experiment: SAXS data collected at BM29, ESRF on 2021 Sep 24
Structural basis for the ligand promiscuity of the neofunctionalized, carotenoid-binding fasciclin domain protein AstaP. Commun Biol 6(1):471 (2023)
Kornilov FD, Slonimskiy YB, Lunegova DA, Egorkin NA, Savitskaya AG, Kleymenov SY, Maksimov EG, Goncharuk SA, Mineev KS, Sluchanko NN
RgGuinier 2.1 nm
Dmax 8.5 nm
VolumePorod 46 nm3

SASDP99 – invariant surface glycoprotein ISG65

65 kDa invariant surface glycoprotein, putative experimental SAS data
invariant surface glycoprotein ISG65 Rg histogram
Sample: 65 kDa invariant surface glycoprotein, putative monomer, 41 kDa Trypanosoma brucei gambiense … protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 25
Cryo-EM structures of Trypanosoma brucei gambiense ISG65 with human complement C3 and C3b and their roles in alternative pathway restriction. Nat Commun 14(1):2403 (2023)
Sülzen H, Began J, Dhillon A, Kereïche S, Pompach P, Votrubova J, Zahedifard F, Šubrtova A, Šafner M, Hubalek M, Thompson M, Zoltner M, Zoll S
RgGuinier 3.3 nm
Dmax 12.5 nm
VolumePorod 53 nm3

SASDPA9 – invariant surface glycoprotein ISG65 bound to complement C3 (C3d)

65 kDa invariant surface glycoprotein, putativeComplement C3 experimental SAS data
DAMMIF model
Sample: 65 kDa invariant surface glycoprotein, putative monomer, 41 kDa Trypanosoma brucei gambiense … protein
Complement C3 monomer, 35 kDa Homo sapiens protein
Buffer: 20 mM HEPES, 150 mM NaCl, 3% (v/v) glycerol, pH: 7.5
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 25
Cryo-EM structures of Trypanosoma brucei gambiense ISG65 with human complement C3 and C3b and their roles in alternative pathway restriction. Nat Commun 14(1):2403 (2023)
Sülzen H, Began J, Dhillon A, Kereïche S, Pompach P, Votrubova J, Zahedifard F, Šubrtova A, Šafner M, Hubalek M, Thompson M, Zoltner M, Zoll S
RgGuinier 3.5 nm
Dmax 12.8 nm
VolumePorod 104 nm3

SASDQ99 – Immunoglobulin G1 (pH 2, 0 M NaCl), SEC-SAXS data

humanized immunoglobulin G1 monoclonal antibody experimental SAS data
humanized immunoglobulin G1 monoclonal antibody Kratky plot
Sample: Humanized immunoglobulin G1 monoclonal antibody monomer, 148 kDa Mouse/human (chimera)
Buffer: 100 mM glycine-HCl, pH: 2
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2016 Mar 2
Getting Smaller by Denaturation: Acid-Induced Compaction of Antibodies The Journal of Physical Chemistry Letters :3898-3906 (2023)
Imamura H, Ooishi A, Honda S
RgGuinier 5.1 nm
Dmax 17.6 nm
VolumePorod 260 nm3

SASDQA9 – Immunoglobulin G1 (pH 2, 0.2 M NaCl), SEC-SAXS data

humanized immunoglobulin G1 monoclonal antibody experimental SAS data
humanized immunoglobulin G1 monoclonal antibody Kratky plot
Sample: Humanized immunoglobulin G1 monoclonal antibody monomer, 148 kDa Mouse/human (chimera)
Buffer: 100 mM glycine-HCl, 200 mM NaCl, pH: 2
Experiment: SAXS data collected at BL-10C, Photon Factory (PF), High Energy Accelerator Research Organization (KEK) on 2017 Mar 5
Getting Smaller by Denaturation: Acid-Induced Compaction of Antibodies The Journal of Physical Chemistry Letters :3898-3906 (2023)
Imamura H, Ooishi A, Honda S
RgGuinier 4.1 nm
Dmax 14.2 nm
VolumePorod 240 nm3

SASDPL4 – Human MUC2 mucin C-terminal dimer

Human Mucin 2 C-terminal experimental SAS data
DAMMIN model
Sample: Human Mucin 2 C-terminal dimer, 240 kDa Homo sapiens protein
Buffer: 25 mM HEPES, 100 mM NaCl, 10 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at BM29, ESRF on 2020 Nov 20
The intestinal MUC2 mucin C-terminus is stabilized by an extra disulfide bond in comparison to von Willebrand factor and other gel-forming mucins Nature Communications 14(1) (2023)
Gallego P, Garcia-Bonete M, Trillo-Muyo S, Recktenwald C, Johansson M, Hansson G
RgGuinier 8.4 nm
Dmax 32.0 nm
VolumePorod 684 nm3

SASDRU7 – Angiotensin-converting enzyme 2/immunoglobulin-Fc chimera protein (Ace2-Fc) at 3.81 mg/ml

Angiotensin-converting enzyme 2 chimera with the Fc region of the immunoglobulin heavy constant gamma 4 experimental SAS data
Angiotensin-converting enzyme 2 chimera with the Fc region of the immunoglobulin heavy constant gamma 4 Kratky plot
Sample: Angiotensin-converting enzyme 2 chimera with the Fc region of the immunoglobulin heavy constant gamma 4 dimer, 217 kDa Homo sapiens protein
Buffer: 50 mM Tris, 150 mM NaCl, pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-1000, SFB 1035, Technische Universität München on 2021 Jul 5
Extrinsic stabilization of antiviral ACE2-Fc fusion proteins targeting SARS-CoV-2. Commun Biol 6(1):386 (2023)
Svilenov HL, Delhommel F, Siebenmorgen T, Rührnößl F, Popowicz GM, Reiter A, Sattler M, Brockmeyer C, Buchner J
RgGuinier 5.5 nm
Dmax 18.6 nm
VolumePorod 408 nm3

SASDMR5 – Tn3 family transposase (TnpA WT)

TnpA transposase experimental SAS data
GASBOR model
Sample: TnpA transposase dimer, 234 kDa Bacillus thuringiensis serovar … protein
Buffer: 50 mM HEPES, 200 mM NaCl, 100 mM L-Arg HCL, pH: 7.9
Experiment: SAXS data collected at SWING, SOLEIL on 2017 Nov 2
AFM-based force spectroscopy unravels stepwise formation of the DNA transposition complex in the widespread Tn3 family mobile genetic elements. Nucleic Acids Res (2023)
Fernandez M, Shkumatov AV, Liu Y, Stulemeijer C, Derclaye S, Efremov RG, Hallet B, Alsteens D
RgGuinier 4.6 nm
Dmax 16.0 nm
VolumePorod 480 nm3

SASDS57 – Heterotrimeric membrane protein complex FtsB-FtsL-FtsQ

Cell division protein FtsBCell division protein FtsLCell division protein FtsQ experimental SAS data
DAMMIN model
Sample: Cell division protein FtsB hexamer, 70 kDa Escherichia coli (strain … protein
Cell division protein FtsL hexamer, 82 kDa Escherichia coli (strain … protein
Cell division protein FtsQ hexamer, 189 kDa Escherichia coli (strain … protein
Buffer: 20 mM HEPES pH 7.5, 200 mM NaCl, 0.2% of Cymal-5, pH: 7.5
Experiment: SAXS data collected at 13A, Taiwan Photon Source, NSRRC on 2022 Sep 23
Structure of the heterotrimeric membrane protein complex FtsB-FtsL-FtsQ of the bacterial divisome Nature Communications 14(1) (2023)
Nguyen H, Chen X, Parada C, Luo A, Shih O, Jeng U, Huang C, Shih Y, Ma C
RgGuinier 5.3 nm
Dmax 16.5 nm
VolumePorod 421 nm3