Small Angle Scattering Biological Data Bank SASBDB

Browse by DISSEMINATION: Published

SASDAB6 – Xylose Isomerase

Sample:	Xylose Isomerase tetramer, 173 kDa Streptomyces rubiginosus protein
Buffer:	20 mM HEPES 200 mM Na2SO4 50 mM K2SO4 500 % v/v D2O 1 mM MgCl2, pH: 6.6
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Dec 10

Correlation Map, a goodness-of-fit test for one-dimensional X-ray scattering spectra. Nat Methods 12(5):419-22 (2015)
Franke D, Jeffries CM, Svergun DI

R_g^Guinier	3.2	nm
D_max	9.1	nm
Volume^Porod	234	nm³

SASDAL6 – Wild-type chalcone isomerase, ligand-free

Bacterial chalcone isomerase experimental SAS data

Bacterial chalcone isomerase Kratky plot

Sample:	Bacterial chalcone isomerase hexamer, 194 kDa Eubacterium ramulus protein
Buffer:	50 mM sodium phosphate, pH: 6.8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23

Structure and catalytic mechanism of the evolutionarily unique bacterial chalcone isomerase. Acta Crystallogr D Biol Crystallogr 71(Pt 4):907-17 (2015)
Thomsen M, Tuukkanen A, Dickerhoff J, Palm GJ, Kratzat H, Svergun DI, Weisz K, Bornscheuer UT, Hinrichs W

R_g^Guinier	4.0	nm
D_max	13.0	nm
Volume^Porod	320	nm³

SASDAM6 – Naringenin-bound chalcone isomerase

Chalcone isomerase with Naringenin experimental SAS data

Chalcone isomerase with Naringenin Kratky plot

Sample:	Chalcone isomerase with Naringenin hexamer, 194 kDa Eubacterium ramulus protein
Buffer:	50 mM sodium phosphate, pH: 6.8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23

R_g^Guinier	3.7	nm
D_max	11.0	nm
Volume^Porod	320	nm³

SASDAN6 – Chalcone isomerase, CHI_Δlid construct

Chalcone isomerase deltaLid experimental SAS data

Sample:	Chalcone isomerase deltaLid hexamer, 181 kDa Eubacterium ramulus protein
Buffer:	50 mM sodium phosphate, pH: 6.8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Sep 23

R_g^Guinier	3.6	nm
D_max	11.0	nm
Volume^Porod	270	nm³

SASDAT6 – Integrin beta4, fragment of the cytoplasmic region encompassing the third and fourth FnIII domains

Sample:	Integrin beta-4 monomer, 23 kDa Homo sapiens protein
Buffer:	20 mM Sodium Phosphate 150 mM NaCl 5% glycerol 3 mM DTT, pH: 7.5
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Aug 6

Combination of X-ray crystallography, SAXS and DEER to obtain the structure of the FnIII-3,4 domains of integrin α6β4. Acta Crystallogr D Biol Crystallogr 71(Pt 4):969-85 (2015)
Alonso-García N, García-Rubio I, Manso JA, Buey RM, Urien H, Sonnenberg A, Jeschke G, de Pereda JM

R_g^Guinier	2.2	nm
D_max	7.0	nm
Volume^Porod	32	nm³

SASDAA7 – Heterotetramer of histidine protein kinase and response regulator

Histidine protein kinaseResponse regulator experimental SAS data

Sample:	Histidine protein kinase dimer, 54 kDa Streptococcus pneumoniae protein Response regulator dimer, 61 kDa Streptococcus pneumoniae protein
Buffer:	20 mM Tris 200 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 7.5
Experiment:	SAXS data collected at Bruker Nanostar, IBBMC on 2012 May 16

Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
Sanchez D, Boudes M, van Tilbeurgh H, Durand D, Quevillon-Cheruel S

R_g^Guinier	4.0	nm
D_max	16.0	nm
Volume^Porod	175	nm³

SASDAB7 – Complex ComE-comcde

comcdeResponse regulator experimental SAS data

Sample:	Comcde, 24 kDa Streptococcus pneumoniae DNA Response regulator dimer, 61 kDa Streptococcus pneumoniae protein
Buffer:	50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2
Experiment:	SAXS data collected at SWING, SOLEIL on 2011 Feb 11

Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
Sanchez D, Boudes M, van Tilbeurgh H, Durand D, Quevillon-Cheruel S

R_g^Guinier	3.4	nm
D_max	10.7	nm
Volume^Porod	122	nm³

SASDAC7 – Complex LytTR-comcde

Sample:	Comcde, 24 kDa Streptococcus pneumoniae DNA Response regulator dimer, 29 kDa Streptococcus pneumoniae protein
Buffer:	50 mM MES 500 mM NaCl 5% (v/v) Glycerol 5 mM β-mercaptoethanol, pH: 6.2
Experiment:	SAXS data collected at SWING, SOLEIL on 2013 Jun 2

Modeling the ComD/ComE/comcde interaction network using small angle X-ray scattering. FEBS J 282(8):1538-53 (2015)
Sanchez D, Boudes M, van Tilbeurgh H, Durand D, Quevillon-Cheruel S

R_g^Guinier	3.6	nm
D_max	12.5	nm
Volume^Porod	74	nm³

SASDBL2 – MBP-PICK1 fusion

Maltose Binding Protein fused to Protein Interacting with C kinase 1 experimental SAS data

Sample:	Maltose Binding Protein fused to Protein Interacting with C kinase 1 dimer, 174 kDa Homo sapiens protein
Buffer:	50 mM Tris 300 mM NaCl 1 mM maltose 1 mM EGTA 2 mM DTT, pH: 7.5
Experiment:	SAXS data collected at G1, Cornell High Energy Synchrotron Source (CHESS) on 2015 Oct 13

PICK1 is implicated in organelle motility in an Arp2/3 complex-independent manner. Mol Biol Cell 26(7):1308-22 (2015)
Madasu Y, Yang C, Boczkowska M, Bethoney KA, Zwolak A, Rebowski G, Svitkina T, Dominguez R

R_g^Guinier	8.4	nm
D_max	27.6	nm
Volume^Porod	483	nm³

SASDLG5 – Human nerve growth factor

Beta-nerve growth factor experimental SAS data

Sample:	Beta-nerve growth factor dimer, 24 kDa Homo sapiens protein
Buffer:	50 mM Na-phosphate, 1 mM EDTA, pH: 7
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2007 Jul 2

The conundrum of the high-affinity NGF binding site formation unveiled? Biophys J 108(3):687-97 (2015)
Covaceuszach S, Konarev PV, Cassetta A, Paoletti F, Svergun DI, Lamba D, Cattaneo A

R_g^Guinier

2.5

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