Small Angle Scattering Biological Data Bank SASBDB

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SASDLJ5 – Aggregation state of tubulin protein

Tubulin alpha-1A chain experimental SAS data

Sample:	Tubulin alpha-1A chain monomer, 50 kDa Homo sapiens protein
Buffer:	phosphate buffered saline (PBS), pH: 7.4
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Dec 3

Interactions of ataxin-3 with its molecular partners in the protein machinery that sorts protein aggregates to the aggresome. Int J Biochem Cell Biol 51:58-64 (2014)
Bonanomi M, Mazzucchelli S, D'Urzo A, Nardini M, Konarev PV, Invernizzi G, Svergun DI, Vanoni M, Regonesi ME, Tortora P

R_g^Guinier	7.0	nm
D_max	25.0	nm
Volume^Porod	340	nm³

SASDLK5 – Aggregation state of ataxin-3-tubulin complex

Ataxin-3Tubulin alpha-1A chain experimental SAS data

Sample:	Ataxin-3 monomer, 41 kDa Homo sapiens protein Tubulin alpha-1A chain monomer, 50 kDa Homo sapiens protein
Buffer:	phosphate buffered saline (PBS), pH: 7.4
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2009 Dec 3

R_g^Guinier	8.4	nm
D_max	30.0	nm
Volume^Porod	900	nm³

SASDLW4 – Human Protein S100-A4

Sample:	Protein S100-A4 dimer, 23 kDa Homo sapiens protein
Buffer:	20 mM HEPES, 20 mM NaCl, 0.1 mM TCEP, pH: 7.5
Experiment:	SAXS data collected at BM29, ESRF on 2013 Mar 2

The C-Terminal Random Coil Region Tunes the Ca2+-Binding Affinity of S100A4 through Conformational Activation PLoS ONE 9(5):e97654 (2014)
Duelli A, Kiss B, Lundholm I, Bodor A, Petoukhov M, Svergun D, Nyitray L, Katona G, Csernoch L

R_g^Guinier	2.1	nm
D_max	7.0	nm
Volume^Porod	48	nm³

SASDDF6 – Myelin basic protein

Myelin basic protein experimental SAS data

Sample:	Myelin basic protein monomer, 18 kDa Bos taurus protein
Buffer:	20 mM NaH2PO4/ Na2HPO4, 99.9% D2O, pH: 4.8
Experiment:	SAXS data collected at BM29, ESRF on 2013 Feb 21

Internal nanosecond dynamics in the intrinsically disordered myelin basic protein. J Am Chem Soc 136(19):6987-94 (2014)
Stadler AM, Stingaciu L, Radulescu A, Holderer O, Monkenbusch M, Biehl R, Richter D

R_g^Guinier	3.3	nm
D_max	11.1	nm

SASDAP4 – Chitinase 60 from Moritella marina

Chitinase 60 from Moritella marina Rg histogram

Sample:	Chitinase 60 monomer, 61 kDa Moritella marina protein
Buffer:	20 mM Tris 200 mM NaCl, pH: 8
Experiment:	SAXS data collected at EMBL P12, PETRA III on 2013 Mar 18

Crystal structures of substrate-bound chitinase from the psychrophilic bacterium Moritella marina and its structure in solution. Acta Crystallogr D Biol Crystallogr 70(Pt 3):676-84 (2014)
Malecki PH, Vorgias CE, Petoukhov MV, Svergun DI, Rypniewski W

R_g^Guinier	3.2	nm
D_max	11.3	nm
Volume^Porod	75	nm³

SASDBF4 – Wild-type LytA (N-His6) with choline

Lytic Amidase with choline experimental SAS data

Sample:	Lytic Amidase with choline dimer, 75 kDa Streptococcus pneumoniae protein
Buffer:	20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2

Structural and functional insights into peptidoglycan access for the lytic amidase LytA of Streptococcus pneumoniae. MBio 5(1):e01120-13 (2014)
Mellroth P, Sandalova T, Kikhney A, Vilaplana F, Hesek D, Lee M, Mobashery S, Normark S, Svergun D, Henriques-Normark B, Achour A

R_g^Guinier	4.9	nm
D_max	15.0	nm
Volume^Porod	115	nm³

SASDBG4 – Prophage LytA with choline

Prophage Lytic Amidase with choline experimental SAS data

Sample:	Prophage Lytic Amidase with choline dimer, 73 kDa Streptococcus pneumoniae protein
Buffer:	20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2

R_g^Guinier	5.9	nm
D_max	17.0	nm
Volume^Porod	130	nm³

SASDBH4 – Wild-type LytA (N-His6) without choline

Lytic Amidase without choline experimental SAS data

Sample:	Lytic Amidase without choline monomer, 37 kDa Streptococcus pneumoniae protein
Buffer:	20 mM Tris 150 mM NaCl 1 µM ZnCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 3

R_g^Guinier	3.4	nm
D_max	12.5	nm
Volume^Porod	56	nm³

SASDBJ4 – Wild-type LytA choline-binding domain

Lytic Amidase choline-binding domain experimental SAS data

Sample:	Lytic Amidase choline-binding domain dimer, 17 kDa Streptococcus pneumoniae protein
Buffer:	20 mM Tris 150 mM NaCl 5 mM choline chloride 1 µM ZnCl2, pH: 7.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Dec 11

R_g^Guinier	3.3	nm
D_max	10.0	nm
Volume^Porod	49	nm³

SASDAR3 – PsrP functional binding region

Functional binding region (187-385) of the pneumococcal serine-rich repeat protein experimental SAS data

PsrP functional binding region Rg histogram

Sample:	Functional binding region (187-385) of the pneumococcal serine-rich repeat protein monomer, 22 kDa Streptococcus pneumoniae protein
Buffer:	20 mM sodium citrate 250 mM NaCl 2.5 % Glycerol, pH: 5.5
Experiment:	SAXS data collected at EMBL X33, DORIS III, DESY on 2011 Jul 2

The basic keratin 10-binding domain of the virulence-associated pneumococcal serine-rich protein PsrP adopts a novel MSCRAMM fold. Open Biol 4:130090 (2014)
Schulte T, Löfling J, Mikaelsson C, Kikhney A, Hentrich K, Diamante A, Ebel C, Normark S, Svergun D, Henriques-Normark B, Achour A

R_g^Guinier	2.3	nm
D_max	7.7	nm
Volume^Porod	37	nm³

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