|
|
|
|
|
| Sample: |
Probable ATP-dependent RNA helicase DDX58 (without CARDs) monomer, 80 kDa Homo sapiens protein
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH: 7.4 |
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 May 29
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
3.7 |
nm |
| Dmax |
13.0 |
nm |
| VolumePorod |
114 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Probable ATP-dependent RNA helicase DDX58 (without CARDs) monomer, 80 kDa Homo sapiens protein
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4 |
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
3.5 |
nm |
| Dmax |
14.0 |
nm |
| VolumePorod |
132 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Probable ATP-dependent RNA helicase DDX58 (without CARDs) monomer, 80 kDa Homo sapiens protein
5´ppp 10mer hairpin dsRNA monomer, 8 kDa RNA
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH: 7.4 |
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 May 29
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
2.8 |
nm |
| Dmax |
9.0 |
nm |
| VolumePorod |
146 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Probable ATP-dependent RNA helicase DDX58 (without CARDs) monomer, 80 kDa Homo sapiens protein
5´ppp 10mer hairpin dsRNA monomer, 8 kDa RNA
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 0.5 mM AMP-PNP, pH: 7.4 |
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 May 29
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
2.8 |
nm |
| Dmax |
9.0 |
nm |
| VolumePorod |
135 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Probable ATP-dependent RNA helicase DDX58 (without CARDs) monomer, 80 kDa Homo sapiens protein
5´ppp 10mer hairpin dsRNA monomer, 8 kDa RNA
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4 |
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
2.8 |
nm |
| Dmax |
8.9 |
nm |
| VolumePorod |
137 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Probable ATP-dependent RNA helicase DDX58 (without CARDs) monomer, 80 kDa Homo sapiens protein
5´ppp 8mer hairpin dsRNA monomer, 6 kDa RNA
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, pH: 7.4 |
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 May 29
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
3.4 |
nm |
| Dmax |
11.6 |
nm |
| VolumePorod |
132 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Probable ATP-dependent RNA helicase DDX58 (without CARDs) monomer, 80 kDa Homo sapiens protein
5´ppp 8mer hairpin dsRNA monomer, 6 kDa RNA
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 0.5 mM AMP-PNP, pH: 7.4 |
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2016 Apr 27
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
3.2 |
nm |
| Dmax |
11.1 |
nm |
| VolumePorod |
114 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Probable ATP-dependent RNA helicase DDX58 (without CARDs) monomer, 80 kDa Homo sapiens protein
5´ppp 8mer hairpin dsRNA monomer, 6 kDa RNA
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4 |
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
3.0 |
nm |
| Dmax |
10.5 |
nm |
| VolumePorod |
132 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
5´ppp 8mer hairpin dsRNA dimer, 13 kDa RNA
Probable ATP-dependent RNA helicase DDX58 (without CARDs) dimer, 160 kDa Homo sapiens protein
|
| Buffer: |
25 mM HEPES, 150 mM NaCl, 2.5 mM MgCl2, 10% glycerol and 1mM DTT, 2mM ADP-AlFx, pH: 7.4 |
| Experiment: |
SAXS
data collected at SAXS/WAXS, Australian Synchrotron on 2015 Nov 20
|
Combined roles of ATP and small hairpin RNA in the activation of RIG-I revealed by solution-based analysis.
Nucleic Acids Res 46(6):3169-3186 (2018)
Shah N, Beckham SA, Wilce JA, Wilce MCJ
|
| RgGuinier |
3.9 |
nm |
| Dmax |
16.0 |
nm |
| VolumePorod |
230 |
nm3 |
|
|
|
|
|
|
|
| Sample: |
Hypothetical protein CTHT_0072540 tetramer, 62 kDa Chaetomium thermophilum protein
|
| Buffer: |
20 mM HEPES, 100 mM NaCl, 2 mM β-mercaptoethanol, pH: 7.5 |
| Experiment: |
SAXS
data collected at EMBL P12, PETRA III on 2013 Jul 30
|
Prp19/Pso4 Is an Autoinhibited Ubiquitin Ligase Activated by Stepwise Assembly of Three Splicing Factors.
Mol Cell 69(6):979-992.e6 (2018)
de Moura TR, Mozaffari-Jovin S, Szabó CZK, Schmitzová J, Dybkov O, Cretu C, Kachala M, Svergun D, Urlaub H, Lührmann R, Pena V
|
| RgGuinier |
4.1 |
nm |
| Dmax |
16.2 |
nm |
| VolumePorod |
145 |
nm3 |
|
|
