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SASDNM4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl pH 7.4, 2.5 mg/mL

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.2 nm
Dmax 26.2 nm

SASDNN4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl pH 7.4, 5 mg/mL

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.1 nm
Dmax 19.8 nm

SASDNP4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl, 30 mM CaCl2, pH 7.4

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 30 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.9 nm
Dmax 22.2 nm

SASDNQ4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl, 60 mM CaCl2, pH 7.4

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 60 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.8 nm
Dmax 22.7 nm

SASDNR4 – Heparan sulphate isolated from porcine mucosa in 50 mM Tris/HCl, 120 mM CaCl2, pH 7.4

Heparan sulphate oligomer experimental SAS data
Heparan sulphate oligomer Kratky plot
Sample: Heparan sulphate oligomer monomer, 20 kDa Sus scrofa domesticus
Buffer: 50 mM Tris-HCl, 120 mM CaCl2, pH: 7.4
Experiment: SAXS data collected at SWING, SOLEIL on 2020 Sep 13
Comparative analysis of heparine oligosaccharides, heparin and heparan sulphate
Adriana Erica Miele
RgGuinier 3.8 nm
Dmax 20.6 nm

SASDXB9 – O’Nyong-Nyong virus (ONNV) nsP4 full length wild type

RNA-directed RNA polymerase nsP4 experimental SAS data
RNA-directed RNA polymerase nsP4 Kratky plot
Sample: RNA-directed RNA polymerase nsP4 monomer, 68 kDa O'nyong-nyong virus protein
Buffer: 25 mM HEPES, 1 mM TCEP, 5% glycerol and 500 mM NaCl., pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Pennsylvania State University on 2023 Jun 8
A fold switch regulates conformation of an alphavirus RNA-dependent RNA polymerase. Nucleic Acids Res 54(2) (2026)
Arnold JJ, Braet SM, Vieira LC, Moustafa IM, Gohara DW, Fecko JA, Su YN, Jain A, Aponte-Diaz D, Wilke CO, Anand GS, Yennawar NH, Cameron CE
RgGuinier 3.6 nm

SASDXC9 – O'Nyong Nyong virus C-terminus-50residues-nsP3-nsP4 fusion protein

50-residue-C-terminus-nsP3 fused with RNA-directed RNA polymerase nsP4 experimental SAS data
50-residue-C-terminus-nsP3 fused with RNA-directed RNA polymerase nsP4 Kratky plot
Sample: 50-residue-C-terminus-nsP3 fused with RNA-directed RNA polymerase nsP4 tetramer, 318 kDa O'nyong nyong virus protein
Buffer: 25 mM HEPES 5% glycerol, 1 mM TCEP, 500 mM NaCl., pH: 7.5
Experiment: SAXS data collected at Rigaku BioSAXS-2000, Pennsylvania State University on 2025 Aug 14
A fold switch regulates conformation of an alphavirus RNA-dependent RNA polymerase. Nucleic Acids Res 54(2) (2026)
Arnold JJ, Braet SM, Vieira LC, Moustafa IM, Gohara DW, Fecko JA, Su YN, Jain A, Aponte-Diaz D, Wilke CO, Anand GS, Yennawar NH, Cameron CE
RgGuinier 5.2 nm

SASDQP9 – Hendra virus W protein

Protein W experimental SAS data
Protein W Kratky plot
Sample: Protein W monomer, 53 kDa Hendra virus (isolate … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Henipavirus W proteins
Frank Gondelaud
RgGuinier 6.8 nm
Dmax 26.0 nm
VolumePorod 250 nm3

SASDQQ9 – Hendra virus P/V/W N-terminal domain of protein W

Protein W experimental SAS data
Protein W Kratky plot
Sample: Protein W monomer, 47 kDa Hendra virus (isolate … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Henipavirus W proteins
Frank Gondelaud
RgGuinier 6.4 nm
Dmax 32.0 nm

SASDQR9 – Hendra W protein C123-only mutant

Protein W mutant (C316S, C334S, C419S) experimental SAS data
Protein W mutant (C316S, C334S, C419S) Kratky plot
Sample: Protein W mutant (C316S, C334S, C419S) dimer, 105 kDa Hendra virus (isolate … protein
Buffer: 20 mM HEPES, 150 mM NaCl, pH: 7.2
Experiment: SAXS data collected at SWING, SOLEIL on 2022 Jul 14
Henipavirus W proteins
Frank Gondelaud
RgGuinier 8.1 nm
Dmax 37.0 nm